TriticeaeMIRdb:The Triticeae Mirna Database

Introduction

TriticeaeMIRdb aims to utilize the genomic information of barley MorexV3, wheat IWGSC, and various wheat families, relying on bioinformatics technology and software to predict and analyze the mature and precursor sequences of miRNAs in each wheat family. Based on a three-layer architecture using MySQL, PHP, and Apache, a comprehensive online database will be established by integrating a large amount of publicly available miRNA dataset information to improve the utilization of these miRNA seq datasets. The database provides basic information query and browsing functions for wheat miRNA seq, and predicted miRNA results can be displayed in various interactive and downloadable formats. Providing powerful tools and laying a theoretical foundation for the study of miRNAs in the wheat family, the key sequence information provided provides new ideas for the research of various wheat families.

Summary of research on various bioprojects

BioProject Submitter Study/Bioproject Title Publication Instrument Description
PRJNA656930 Qinghai university Hordeum vulgare Raw sequence reads Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection Illumina HiSeq 2500 Raw data of miRNAs in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection.
PRJNA237744 University of Warwick Hordeum vulgare strain:Qasr Ibrim strain Targeted Locus (Loci) A complete ancient RNA genome: identification, reconstruction and evolutionary history of archaeological Barley Stripe Mosaic Virus Illumina HiSeq 2500 sRNA coverage of several genomic loci in archaeological barley (Qasr Ibrim strain) and modern control samples.
PRJNA529516 Genebang Hulless Barley SmallRNA Sequencing Identification and Characterization of Downy Mildew-Responsive microRNAs in Indian Vitis vinifera by High-Throughput Sequencing Illumina HiSeq 2500 Identification and characterization of nitrogen-responsive microRNAs in Tibetan hulless barley by high-throughput sequencing.
PRJNA507337 Zhejiang Univerisity Small RNA and degradome sequencing of cultivated barley and Tibetan wild barley Differential changes in grain ultrastructure, amylase, protein and amino acid profiles between Tibetan wild and cultivated barleys under drought and salinity alone and combined stress. Illumina HiSeq 2500 Using small RNA sequencing and degradome analysis to identify microRNAs in response to salt stress in cultivated and Tibetan wild barley.
PRJNA823894 Henan Agricultural University Small RNA sequencing of virus-infected barley plants Barley GRIK1-SnRK1 kinases subvert a viral virulence protein to upregulate antiviral RNAi and inhibit infection Illumina HiSeq 2500 To investigate the function of HvSDN1 in regulating the BYDV derivative vsiRNA accumulation, the barley plants with silencing HvSDN1 using BSMV-VIGS (BSMV-SDN1gs) and its control plants (BSMV-EV) were further employed to inoculate the BYDV-GAV, thus producing four groups of plants, i.e., BSMV-EV/BYDV- (without HvSDN1 silencing and free of BYDV), BSMV-SDN1gs/BYDV- (with HvSDN1 silenced and free of BYDV), BSMV-EV/BYDV+ (without HvSDN1 silencing and infected by BYDV), BSMV-SDN1gs/BYDV+ (with HvSDN1 silenced and infected by BYDV). The small RNAs were extracted from the four types of plants at 21 dpi of BYDV and were used to construct the small RNA-Seq library.
PRJNA823841 Henan Agricultural University Small RNA sequencing of BYDV-infected barley plants Barley GRIK1-SnRK1 kinases subvert a viral virulence protein to upregulate antiviral RNAi and inhibit infection Illumina HiSeq 2500 To examine if the elevation of antiviral defense displayed in transgenic barley plants that overexpressing SnRK1-YFP might be associated with the increased abundance of BYDV vsiRNAs, small RNA sequencing assays were conducted to analyze the vsiRNA accumulation levels in BYDV-infected transgenic barley plants and WT controls. The small RNAs were separated from the total RNAs that extracted from the BYDV-infected barley plants at 4, 7 and 14 dpi, and then were employed for constructing the small RNA-Seq library.
PRJNA816439 Shiraz University miRNA-barley - Illumina HiSeq 2500 miRNA from illumina platform, SAM at double ridge and triple mound stage, barley, cultivar Morex.
PRJNA809109 Imperial College London small RNA from barley powdery mildew Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction NextSeq 550 The aim of this study is to determine the small RNA (sRNA) produced by barley and Blumeria graminis f. sp. hordei. Infected leaves were dissected to separate infection structures, and extracellular vesicles from the plant apoplast. The samples analysed were: epiphytic mycelium and spores; infected barley epidermis; haustoria; microsomes from infected epidermis; extracellular vesicles from infected or non-infected barley leaves.
PRJEB40118 CURTIN UNIVERSITY Small RNA sequencing of a barley powdery mildew susceptible cultivar (cv) Baudin near-isogenic lines (NILs) introgressed with mlo-11 and mlo-11 (cnv2) mutations Physiological Changes in Barley mlo-11 Powdery Mildew Resistance Conditioned by Tandem Repeat Copy Number Illumina HiSeq 2500 Wild barley accessions have evolved broad-spectrum defence against barley powdery mildew through naturally occurring recessive mlo mutations. This research elucidates the pronounced repression of the Mlo promoter in mlo-11 compared to mlo-11 (cnv2) was governed by 24 nt heterochromatic small interfering RNAs.
PRJNA691038 Instytut Hodowli i Aklimatyzacji Roślin - PIB Barley seeds miRNome stability during long-term storage and aging Barley Seeds miRNome Stability during Long-Term Storage and Aging Illumina MiSeq Seed aging is a complex biological process attracting the scientists' attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that despite the same genetic makeup differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families i.e. miR159, miR156, miR166 and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along to the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability was detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state. It might be responsible for the removal of (1-3.1-4)-beta-D-glucanase transcripts and lowering or completely blocking the synthesis of this key enzyme for seed germination. Overall design: Examiantiopn of 3 barley seeds stocks: Rc - after seed regeneration i.e. non-stored control; Lv - seeds after storage 1972-2018 (germination 2%); Hv - seeds after storage 1972-2018 (germination 86,7%).
PRJNA682179 zhejiang university Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq] BIdentification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated Illumina HiSeq 2500 Soil potassium deficiency has become a global problem in agricultural production, seriously restricting crops productions and agricultural sustainable development. Identification of the microRNAs and understanding their functions in response to low K stress will be helpful for developing crop varieties with low K tolerance. Our previous study identified a low K tolerant accession XZ153 from Tibetan wild barley. In this study, small RNA and degradome analysis were performed on two barley genotypes differing in low K tolerance (XZ153, tolerant; ZD9, sensitive) to identify the miRNAs and their targets responding to low K stress. A total of 1108 miRNAs were detected in shoots of XZ153, and ZD9 at 2 d and 7 d after low K stress, and their targets were identified through bioinformatics prediction and degradome analysis. We identified 65 differentially expressed miRNAs responding to low K stress. The results also showed that miR164c, miR169h and miR395a modules could mediate TCA cycle, glycolysis pathway and pentose phosphate pathway responding to low K stress. The osa-miR166g-3p and ghr-miR482b may act as the regulators in Ca2+ signaling pathway in response to low K stress. The methionine salvage cycle involved in ethylene biosynthesis process mediated by miR396c-3p and osa-miR171e-5p might be also involved in responding to low K stress. Some miRNAs, including miR160a, miR396c and miR169h modules, which participated in photosynthesis regulation under low K stress, differed between the two barley genotypes. In conclusion, these exclusively expressed miRNAs and their targets play the crucial roles in low K tolerance. Overall design: A total of 24 shoot samples (two genotypes x two potassium conditions x two time periods x three replications) were harvested for further RNA isolation and library construction.
PRJNA636099 Donald Danforth Plant Science Center sRNA and RNA sequencing profile of barley and wheat anther Premeiotic, 24-Nucleotide Reproductive PhasiRNAs Are Abundant in Anthers of Wheat and Barley But Not Rice and Maize NextSeq 550 Two classes of pre-meiotic (21-nt) and meiotic (24-nt) phasiRNAs and their patterns of accumulation, have been described in maize and rice anthers. Their precise function remains unclear but some studies have shown that they support male fertility. Their important role in anthers underpins our current study to characterize phasiRNAs in wheat and barley anthers. We staged anthers at every 0.2 mm of development for one wheat and two barley varieties. We identified pre-meiotic (0.2 mm, 0.4 mm and 0.6 mm), meiotic (0.8 mm, 1.0 mm and 1.4 mm) and post-meiotic (1.8 mm) anthers for which we then investigated accumulation patterns of RNAs, including reproductive phasiRNAs.
PRJNA634514 Laval university Small RNA (sRNA) and parallel analysis of RNA ends (PARE) analysis during the early stage of gametic embryogenesis in barley The commitment of barley microspores into embryogenesis correlates with miRNA-directed regulation of members of the SPL, GRF and HD-ZIPIII transcription factor families Illumina HiSeq 2500 Small RNA (sRNA) and parallel analysis of RNA ends (PARE) sequencing data. The scope of this data consists to study the regulatory activity of micro RNA (miRNA) during the early stages of microspore development in gametic embryogenesis in barley using the embryogenic-responsive cultivar Gobernadora.
PRJNA608272 Departemnt of Gene Expression, Adam Mickiewicz University in Poznan NGS analysis of small RNAs under nitrogen excess stress in shoot and root of 2 week old barley plants Expression Analysis of Nitrogen Metabolism-Related Genes Reveals Differences in Adaptation to Low-Nitrogen Stress between Two Different Barley Cultivars at Seedling Stage Illumina HiSeq 4000 In order to identify microRNAs involved in response to nitrogen excess stress we performed deep sequencing of sRNA libraries constructed form RNA isolated from roots and shoots of two week old barley plants that have been grown under nitrogen excess stress and control conditions. Overall design: barley plants grown under conditions and nitrogen excess stress small RNA sequencing.
PRJNA607205 Adam Mickiewicz University, Poznan Deep-sequencing of small RNAs from barley shoot and root growing in low-Pi and control condition Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses Illumina HiSeq 4000 RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. We used a modified method that allows for enrichment of small RNAs. Libraries were prepared using TruSeq Small RNA Library Preparation Kit (Illumina), followed by sequencing of NGS libraries. Overall design: Total RNA isolation, libraries preparation, single-reads (50 cycles) sequencing, Illumina technology, HiSeq 4000, sequencing performed at Fasteris SA (Switzerland).
PRJNA607200 Adam Mickiewicz University, Poznan Deep-sequencing of degraded or cleaved RNAs from barley shoot and root growing in low-Pi condition Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses Illumina HiSeq 2500 RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries. Overall design: Total RNA isolation, PARE libraries preparation, single-reads (50 cycles) sequencing, Illumina technology, HiSeq 2500, sequencing performed at Fasteris SA (Switzerland).
PRJNA476756 CICG, USDA/ARS Small RNA sequencing of Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei (Bgh); isolate 5874) Biological Roles of Small Rnas Expressed During Infection of Barley by the Obligate Fungal Biotroph, Blumeria graminis f. sp. hordei Illumina HiSeq 2500 The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012. Molecular Plant Pathology 13 (4): 414-430. DOI: 10.1111/j.1364-3703.2011.00783.x). Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to identify small RNAs (sRNAs) from both barley and Bgh that regulate gene expression both within species and cross-kingdom. Overall design: 90 samples analyzed = 5 genotypes * 6 time points * 3 replications Note: This experiment used the identical split-plot design, tissue, and source RNA as GEO submission # 101304 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE101304).
PRJNA526135 Adam Mickiewicz University Poznan miRNA expression during development and drought stress in barley A Functional Network of Novel Barley MicroRNAs and Their Targets in Response to Drought Illumina HiScanSQ Combating loss of production due to drought stress is especially important for barley cultivation. With increasing number of data confirming the importance of miRNA-mediated regulation, we have searched for novel barley miRNAs related to drought stress. We performed deep sequencing of small RNAs isolated from developmental stages of barley plants as well as drought stressed plants coupled with degradome analysis.
PRJNA485436 College of Agriculture &Biotechnology, Zhejiang Un A wild barley accession WB-1 and a barley cultivar Golden Promise Raw sequence reads Identification of microRNAs Responding to Aluminium, Cadmium and Salt Stresses in Barley Roots Illumina HiSeq 2500 16 small RNA libraries of shoot and root tissues from a wild barley accession (WB-1) and a cultivar Golden Promise with contrasting Cd tolerance were constructed and sequenced. Moreover, a degradome library was also conducted to identify the target genes of miRNAs.
PRJNA481620 Zhejiang University Hordeum vulgare Genome sequencing Identification of microRNAs in response to aluminum stress in the roots of Tibetan wild barley and cultivated barley Illumina HiSeq 2500 Up to date, there is no report about sequencing and identification of miRNA responsive to Al stress in barley. Thus, this study could provide useful information for revealing molecular mechanism of miRNAs in regulating Al tolerance in barley.
PRJDB6438 NICS_NARO Cloning and Functional Analysis Agronomically Important Genes on Barley miR172 downregulates the translation of cleistogamy 1 in barley Illumina Genome Analyzer IIx Cultivars of barley (Hordeum vulgare L.) showing a cleistogamous floret phenotype hold an ability to prevent the plants from infection of various invasive fungi, notably certain Fusarium ssp., and also greatly reduce the risk of gene flow through pollen dispersal. A single recessive gene cleistogamy 1 (cly1) encoding an APETLA2 (AP2) type protein determines the cleistogamy in barley. Within the cly1 gene, sequence variations at the miR172 target site are associated with the lodicule development and then the cleistogamous phenotype. An miRNA library from immature spikes of barley variety Morex is constructed and sequenced to characterize the interaction of miRNA with cly1 gene sequences and indetify the barley miR172 genes through sequence alignment against the Morex whole genome sequence for understanding of the regulation mechanisms on cly1 gene activity as well as its gene product, AP2 protein.
PRJNA389146 Zhejiang University Sequencing of barley embryonic microRNAs microRNAs participate in gene expression regulation and phytohormone cross-talk in barley embryo during seed development and germination Illumina HiSeq 2000 Sequencing of barley embryonic microRNAs at three different stages: Seed-1DAG, Seed-5DAG and Embryo-10DPA.
PRJNA354889 Northwest A&F University Hordeum vulgare subsp. vulgare Transcriptome or Gene expression Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.) Illumina HiSeq 2000 Barley is a well-studied model of cereal grain development and maturation. To better understand the molecular basis for barley grain development, we performed a comparative study of transcriptome and small RNAs dynamics between four developmental phases of this process: early pre-storage phase (0-5 DAP), late pre-storage or transition phase (6-10 DAP), early storage phase (11-15 DAP) and levels off stages (16-20 DAP).
PRJNA294348 Northwest A&F University Hordeum vulgare subsp. vulgare Transcriptome or Gene expression Genome-Wide Identification and Characterization of Salinity Stress-Responsive miRNAs in Wild Emmer Wheat (Triticum turgidum ssp. dicoccoides) Illumina HiSeq 2000 sRNA sequencing of barley under salt stress
PRJNA960906 University of Washington Small RNA sequencing of wheat infected with stem rust Dual host-pathogen small RNA sequencing during wheat stem rust infection Ion Torrent Proton This experiment isolated small RNA from Triticum aestivum seedlings infected with Puccinia graminis f.sp. tritici, in order to study gene regulation during a biotrophic interaction. Three infected replicates and three mock-inoculated replicates. Libraries were sequenced on the Ion Torrent platform.
PRJNA929049 Kastamonu University miRNA sequencing of three Turkish wheat species Diverse expression pattern of wheat transcription factors against abiotic stresses in wheat species Illumina HiSeq 2000 This is a comparison study about miRNA libraries of three Turkish wheat cultivars in different ploidy levels (Triticum aestivum cv. Yuregir-89, Triticum aestivum cv. Kiziltan-91 and Triticum monococcum). In this project, it was aimed to reveal the miRNAs that play a role in drought stress tolerance by investigating the differences in miRNA expression profiles under drought stress among one drought tolerant (Kiziltan-91), another moderately drought tolerant (Yuregir-89) and ancestral wheat species. For this purpose, total RNA isolation was performed by collecting root and leaf samples from control and stressed plants and sRNA-Seq was performed using next generation sequencing (NGS) technology.
PRJNA916207 National Agri food biotechnology institute System analysis of differentially expressed miRNAs in hexaploid wheat display tissue-specific regulatory role during Fe deficiency response System analysis of differentially expressed miRNAs in hexaploid wheat display tissue-specific regulatory role during Fe deficiency response Illumina NovaSeq 6000 Iron (Fe) deficiency in soil could largely affect crop productivity but the molecular mechanisms underlying the regulation of Fe deficiency response are still limited. Specifically, microRNA (miRNA) mediated regulation of Fe deficiency genes and the network is poorly understood. In the current work, we aim to understand the molecular mechanisms of Fe deficiency response in wheat seedlings by generating an inventory of differentially expressed miRNAs. Stem loop quantitative RT PCR analysis suggested the expression of these miRNAs in a spatio-temporal fashion, with few showing time-specific expression responses. Furthermore, miRNA target prediction analysis suggested that few of these miRNA could specifically target genes such as multicopper oxidases, E3 ubiquitin ligases, GRAS family and WRKY transcription factors known to be involved in Fe homeostasis. Our work provides insights into miRNA-mediated regulatory pathways during Fe deficiency response. The first information generated here will help to identify the candidate genes for genetic improvement of Fe deficiency tolerance in hexaploid wheat.
PRJNA895954 Anhui Agricultural University Whole transcriptome resequencing for temperature-mediated seed dormancy regulation in wheat Identification and validation of coding and non-coding RNAs involved in high-temperature-mediated seed dormancy in common wheat Illumina HiSeq 4000 Identification of mRNAs lncRNAs and miRNAs involved in temperature-mediated seed dormancy regulation in wheat
PRJNA877088 Korea university Wheat spikelet total mRNA sequencing Heat-induced RING/U-BOX E3 ligase, TaUHS, is a negative regulator by facilitating TaLSD degradation during the grain filling period in wheat HiSeq X Ten Analysis total RNA expression under heat stress during grain-filling stage
PRJNA867659 Chinese Academy of Agricultural Sciences Analysis of small RNA in wheat ovary stages infected by Tilletia laevis Kuhn Characteristics of the Infection of Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) in Compatible Wheat Illumina HiSeq 2500 We used a high-throughput sequencing approach to determine miRNAs in three different developmental stages of T.laevis-infected wheat ovaries.
PRJDB11752 CU_KERALA sRNA transcriptome of wheat in response to stem rust and leaf rust interaction Stem and leaf rust-induced miRNAome in bread wheat near-isogenic lines and their comparative analysis Illumina HiSeq 2500 The R-gene mediated resistance has been employed as an efficient approach to control the rust diseases in wheat, one of the dreadful biotrophic fungal diseases affecting crop yield. The R-genes Sr36 and Lr45 have proven their ability in controlling highly virulent strains of stem rust and leaf rust diseases respectively. However, these R-gene mediated modulation over the regulatory non-coding RNAs was unclear. We demonstrated the alteration in non-coding RNA profiles during the resistant and susceptible interaction of wheat with stem rust and leaf rust using Sr36 and Lr45 carrying lines as resistant lines. Puccinia graminis pathotype 40A and Puccinia triticina pathotype 77-5 were used for the infection treatment whereas, water was used for the mock treatment. The non-coding RNA profiles obtained in this study will be a resource to the expanding rust disease management program.
PRJNA289147 Scot Hulbert,Washington State University Triticum aestivum infected with Puccinia striiformis : small RNA and PARE (degradome) sequencing Analysis of miRNAs in Two Wheat Cultivars Infected With Puccinia striiformis f. sp. tritici Ion Torrent Proton,Illumina HiSeq 2500 The fungus Puccinia striiformis causes stripe rust disease in cereal crops. This project contains raw sequence reads from small RNA sequencing and Parallel Analysis of RNA Ends (PARE, i.e. degradome-seq). Both infected and uninfected wheat are featured.
PRJNA837867 Shanxi Agricultural University Triticum aestivum Raw sequence reads Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) Illumina NovaSeq 6000 Drought induced miRNAome of two wheat (Triticum aestivum L.) varieties.
PRJNA799639 Northwest A&F University Object to identify drought memory-related miRNA in wheat Integrate Small RNA and Degradome Sequencing to Reveal Drought Memory Response in Wheat (Triticum aestivum L.) Illumina HiSeq 2500 This study identified drought memory response miRNAs and their target genes in wheat by small RNA.Trileaf stage wheat seedlings were divided into three groups: control group (CG), direct drought (DD) and drought memory (DM) groups.
PRJNA791687 Biology Centre ASCR, v.v.i. Transcriptome of Triticum aestivum genotypes associated with resistance against the Wheat dwarf virus Transcriptome Dynamics in Triticum aestivum Genotypes Associated with Resistance against the Wheat Dwarf Virus NextSeq 500 Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived Triticum aestivum transcriptome (RNA-seq) profiling methods and to evaluate genotypes associated with resistance against the Wheat dwarf virus. Methods: Triticum aestivum mRNA profiles of genotypes associated with resistance against the Wheat dwarf virus were generated by deep sequencing, in four replicates, using Illumina. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows-Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT-PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: Our study represents the first detailed analysis of Triticum aestivum transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA and miRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions. Overall design: Triticum aestivum mRNA profiles of genotypes associated with resistance against the Wheat dwarf virus were generated.
PRJNA698286 School of Resources and Environment Triticum aestivum Raw sequence reads Comparative Small RNA Profiling and Functional Exploration on Wheat With High- and Low-Cadmium Accumulation HiSeq X Ten To find out the miRNAs related to the absorption of heavy metal cadmium in wheat.
PRJNA683746 Yangzhou University sRNA-seq and RNA-seq of wheat inoculating Fusarium graminearum Identification of Wheat LACCASEs in Response to Fusarium graminearum as Potential Deoxynivalenol Trappers BGISEQ-500 Elucidate the mechanism of wheat-Fusarium graminearum interaction.
PRJNA656372 Life sciences college, Henan Normal University Temporal expression study of microRNAs in crown tissues of winter wheat grown under natural growth condition Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions BGISEQ-500 MicroRNAs (miRNAs) are single strand small non-coding RNAs that regulate target mRNAs at post-transcription level. Winter wheat (Triticum aestivum L.), is an important crop plant all over the world. Long term cold exposure (vernalization) is necessary for winter wheat transition from vegetative growth to reproductive growth, yet the involvement of miRNAs in these stages remains unknown. Therefore, we performed next generation sequencing of small RNAs profiles in crown tissues at three-leaf stage, winter dormancy stage, spring greenup stage and jointing stage. Overall design: To identify miRNAs involved in winter wheat growth and development in te field, winter wheat cultivar Shimai 22 was grown in the field under natural weather condition. Crown tissues were collected at 4 different growth and development stages: three leaf stage, winter dormancy stage, spring greenup stage and jointing stage, small RNA libraries were constructed, and BGISEQ500 RS (BGI, China) sequencing platform was used for small RNA libraries sequencing.
PRJNA642367 Agricultural Genomics Institute at Shenzhen, China The impacts of m6A methylation on mRNA translation of wheat Transcriptome-wide analyses of RNA m6A methylation in hexaploid wheat reveal its roles in mRNA translation regulation Illumina HiSeq 2500 To investigate the roles of m6A methylation in the regulation of mRNA translation of wheat genes. We conducted transcriptome profiling of m6A and small RNA, and measured the translation levels of mRNAs in wheat spikes. The translation efficienies were compared between mRNAs with or wihtout m6A.
PRJNA578261 Northwest A&F University Wheat anther miRNA sequencing TaEXPB5 functions as a gene related to pollen development in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm HiSeq X Ten To reveal the mechanism of thermosensitive male sterility in wheat.
PRJNA563099 Henan Agriculture University miRNA in wheat response to different N level Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels Illumina HiSeq 2000 Study the key miRNA related to nitrogen stress during wheat develoment.
PRJNA553193 University of Zurich Small RNA-seq from hexaploid bread wheat (Triticum aestivum) infected with wheat powdery mildew Domestication of High-Copy Transposons Underlays the Wheat Small RNA Response to an Obligate Pathogen Illumina HiSeq 2500 Sequencing data from wheat leaf material infected with three different powdery mildew (B.g. tritici ) isolates and from uninfected leaves. The goal of the project is to investigate the wheat small RNA response to wheat powdery mildew infection.
PRJNA554945 Henan Agricultural University Triticum aestivum Raw sequence reads Enhanced Senescence Process is the Major Factor Stopping Spike Differentiation of Wheat Mutant ptsd1 Illumina HiSeq 2500 Transcriptomes and miRNomes of the wide type (WT) and mutant ptsd1 at the early spike differentiation stage.
PRJNA551738 Henan Agricultural University The miRNA-seq of three tissues for Guomai 301 (Triticum aestivum) Gene Expression Profiles and microRNA Regulation Networks in Tiller Primordia, Stem Tips, and Young Spikes of Wheat Guomai 301 Illumina HiSeq X Ten To find gene expression profiles of tiller primordia, stem tips and young spikes for Guomai 301 (Triticum aestivum), we carried out the miRNA-seq.
PRJNA516200 Henan Normal University Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the Field BGISEQ-500 MicroRNAs (miRNAs) are small non-coding RNAs that regulate target mRNAs by inducing degradation or preventing translation of their target mRNAs. Winter wheat, Triticum aestivum., is an important crop plant, yet there are only a few studies on the association of miRNAs and growth and development of winter wheat grown in the field. Here we carried out experimental analysis of miRNAs in wheat leaves by analyzing small RNA profiles at different growth stages. Overall design: To identify miRNAs involved in winter wheat growth and development, we performed high-throughput sequencing of sRNA libraries from leaves at 4 different growth and development stages: three leaf stage, winter dormancy stage, spring greenup stage and jointing stage.Winter wheat cultivar Shimai 22 was grown in the field under natural weather condition;BGISEQ500 RS (BGI, China)platform was used.
PRJEB28454 ROTHAMSTED RESEARCH Analysis of small RNA silencing in Zymoseptoria tritici - wheat interactions Analysis of small RNA silencing in Zymoseptoria tritici - wheat interactions Illumina HiSeq 2000 Deep sequencing of small RNA preparations from Zymoseptoria tritici isolate IPO323 cultured in vitro (in Czapek-Dox Broth, CDB) and those from the infected wheat cv. Bobwhite leaf tissues sampled at the four different time points corresponding to the four critical phases of Septoria tritici blotch disease development, namely 4 dpi (asymptomatic phase), 9 dpi (transition to necrotrophic stage), 13 dpi (necrotrophic phase), and 21 dpi (profuse asexual sporulation) was carried out on an Illumina HiSeq 2000 using 50 cycle single end reads. Deep sequencing of small RNA preparations from mock-inoculated wheat cv. Bobwhite leaves sampled at 4 dpi, 9 dpi, 13 dpi, and 21 dpi and from healthy, untreated 17 days old wheat cv. Bobwhite leaves was also carried out using the same sequencing technology.
PRJNA523507 Hebei Agricultural University microRNA to patassium deprivation of wheat Global identification and characterization of miRNA family members responsive to potassium deprivation in wheat (Triticum aestivum L.) Illumina HiSeq 2500 To provide insights into micorRNA to patassium deprivation in the seeding stage of wheat.
PRJNA514418 Nanjing Agriculture University Mutation of a histidine-rich calcium-binding protein gene in wheat confers resistance to Fusarium head blight Mutation of a histidine-rich calcium-binding-protein gene in wheat confers resistance to Fusarium head blight Illumina HiSeq 2500 We isolated the major-effect wheat QTL, Qfhs.njau-3B, that confers head blight resistance and showed that it is the same as the previously designated Fhb1. Fhb1 results from a rare deletion involving the 3' exon of the histidine-rich calcium-binding protein gene on chromosome 3BS. Both wheat and Arabidopsis transformed with the Fhb1 sequence showed enhanced resistance to F. graminearum infection. The translation products of this gene's homologs among plants are well conserved and might be essential to plant growth and development. Fhb1 could be useful not only for curbing FHB diseases in grain crops but also for improving other plants vulnerable to Fusarium spp.
PRJNA511811 Northwest Agriculture & Forestry University sRNA sequencing of wheat infected with BYDV-GAV Barley yellow dwarf virus-GAV-derived vsiRNAs are involved in the production of wheat leaf yellowing symptoms by targeting chlorophyll synthase BGISEQ-500 Obtaining total sRNA data from wheat leaves infected with BYDV-GAV.
PRJNA480952 ETH Zurich Small RNA sequncing of Wheat infected by Zymoseptoria tritici during the infection cycle Small RNA Bidirectional Crosstalk During the Interaction Between Wheat and Zymoseptoria tritici NextSeq 500 We studied the bidirectional cross-kingdom RNAi in wheat-Z. tritici pathosystem. The whole dataset contains sRNA-seq, degradome sequencing and mRNA-seq. The sRNA-seq samples include wheat infected by Z. tritici at 7dpi, 12dpi and 14dpi, wheat without infections (Mocks) at 7dpi, 12dpi and 14dpi, and Z. tritici grow in vitro. All of the sRNA-seq samples have three replicates. The degradome sequencing data include mocks (7dpi and 12dpi), wheat infected by Z. tritici (7dpi and 12dpi) and Z. tritici grow in vitro. Additionally, there are as well mRNA-seq of mocks at 7dpi and 12dpi, which have three replicates.
PRJEB24307 CREA Transcriptomic analysis of Fusarium graminearum disease response in near-isogenic hexaploid wheat lines differeing for the presence of a resistance QTL on chromosome 2DL Comparative Transcriptome Profiles of Near-Isogenic Hexaploid Wheat Lines Differing for Effective Alleles at the 2DL FHB Resistance QTL Illumina Genome Analyzer IIx To mine the molecular response associated with the wheat 2DL FHB resistance QTL and to identify candidate genes implicated in such esistance, a comprehensive transcriptomic analysis of the early response to F. graminearum infection in spikelet and rachis was performermed with the RNA-Seq and miRNA-Seq techniques. The analyses were conducted on two near isogenic lines differing for the presence of the 2DL QTL. The response to fungal infection in terms of mRNAs accumulation trend was similar in both NILs, even if at higher intensity in the susceptible NIL, and involved inhibition of primary metabolism and activation of secondary metabolism, amino acid metabolism, cell all reinforcement and remodelling, scavenging of ROS, hormone metabolism and signalling, detoxification and induction of several genes encoding transcription factors and proteins implicated in pathogens resistance response. To search for candidate genes with expression profiles associated with the 2DL QTL resistance led to the discovery of two different strategies associated with sugar signalling in the two NILs. Two genes associated with FHB suscaptibility were discovered. Altered expression was also observed for fungal non-coding RNAs which putative targets were represented by the WIR1A gene, involved in resistance response, and a gene encoding for a jacalin-related lectin protein, which partecipate in biotic and abiotic stress response. The data support the presence of a cross-talk between the plant and the fungus.
PRJNA420197 Shandong University sRNA sequencing of wheat introgression lines treated by saline or alkaline stress Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat Illumina HiSeq 2000 To identify sRNA relative to saline/alkaline stress in wheat.
PRJNA353130 China Agricultural University Global studies of miR021b function in wheat Wheat miR9678 Affects Seed Germination by Generating Phased siRNAs and Modulating Abscisic Acid/Gibberellin Signaling Illumina HiSeq 2500 Seed germination is not only a critical developmental step in the wheat life cycle, but is also important for agricultural production including yield and quality. However, in wheat, the knowledge of the mechanism of regulating seed germination is still limited. In this study, we found 22nt microRNA (miR) miR021b, specifically expressed in scutellum of developing and germinating wheat seed, generated phased ta-siRNAs by cleaving a long non coding RNA LNCR. Overexpression of miR021b in wheat showed a retarded germination and improved resistance to pre-harvest sprouting (PHS), while its silencing enhanced germination rate through transiently expressing in immature embryos. To figure out the mechanism of miR021b regulating seed germination, we found miR021b affected the expression of genes involved in bioactive gibberellin (GA) synthesis and its overexpression reduced the bioactive GA content and inhibited amylase genes expression. In addition, it was observed that TaVp1, TaABF and TaABI3, responded the abscisic acid (ABA) signaling, can bind the promoter of miR021b precursor and regulated its expression, suggesting that miR021b might function in GA-ABA balance during germination. This study identified a signaling pathway that miR021b controlled GA-dependent seed germination in wheat through generating phased ta-siRNAs by cleaved a long non coding RNA LNCR.
PRJNA392981 Capital Normal University Triticum aestivum Raw sequence reads Genome-Wide Identification and Characterization of Long Non-Coding RNA in Wheat Roots in Response to Ca2+ Channel Blocker Illumina HiSeq 2500 Search different expression mRNA in wheat root induced by Ca2+-channel blocking.
PRJNA383333 Chinese Academy of Agricultral Sciences Micro-RNA sequencing of four different stages of spike in wheat Transcriptome Profiling of Wheat Inflorescence Development from Spikelet Initiation to Floral Patterning Identified Stage-Specific Regulatory Genes Illumina HiSeq 2000 Micro-RNA sequencing of four different stages (DR,FM,AM,TS) of spike in wheat.
PRJNA380529 Harbin Normal University Triticum aestivum Transcriptome or Gene expression Uncovering key small RNAs associated with gametocidal action in wheat Illumina Genome Analyzer Two small RNA libraries were constructed from T. aestivum cv. Chinese Spring (CS) and Chinese Spring-gametocidal 3C chromosome monosomic addition line (CS-3C) and sequenced using Illumina high throughput sequencing technology to investigate whether small RNAs play a role in the gametocidal phenomenon.
PRJNA374855 Instituto de Biotecnologia, INTA sRNAs from Triticum aestivum and Delphacodes kuscheli Raw sequence reads Mal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper Vector Illumina HiSeq 1500 Comparative analysis of sRNAs in MRCV (Mal de Río Cuarto virus) infected plants (wheat) and insects (D. kuscheli).
PRJNA353049 Institute of Genetics and Developmental Biology, C Transcriptome and Epigenome analysis in Polyploidy Wheat and its progenitors New insights on the evolution of nucleolar dominance in newly resynthesized hexaploid wheat Triticum zhukovskyi Illumina HiSeq 2000 We analyzed sequence features of intergenic spacer (IGS) in the progenitors of common wheat Triticum aestivum (AABBDD), which revealed nucleolar organizing region (NOR) dominance hierarchy (BB > DD > AA). The hierarchy is based on the length, transcript start site, number and sequence variation of Rep1 (promoter-like repeat) unit. Transcriptome and epigenome in the synthetic tetraploid wheat (AADD / DDAA) and their progenitors T. urartu (AA) and Ae. tauschii (DD) were further investigated to explore the mechanism for NOR silencing. We found that long non-coding RNAs and 24-nt small interfering RNAs were highly abundant in the IGS regions of the DD, and scarcity in AA. After polyploidy formation, NORs of AA subgenome were silenced and associated with increased levels of 24-nt siRNAs derived from active NOR of the DD subgenome. These DD-subgenome 24-nt siRNAs may trans-act on AA-genome IGS to induce DNA methylation via RNA directed DNA methylation (RdDM). Furthermore, BS-Seq and MeDIP-Seq assay show siRNA-targeted regions in AA-subgenome IGS were hypermethylated. Additionally, the increased H3K27me3 and decreased H4K12ac were found in AA-subgenome IGS after polyploidization. This similar phenomenon of BB 24-nt siRNAs trans-act on DD was detected in hexaploid wheat.
PRJNA347868 Tianjin Institute of Agricultural Quality Standard Triticum aestivum Raw sequence reads GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds Illumina HiSeq 2500 In this study, we performed deep sequencing of sRNAs from a transgenic wheat line overexpressing GmDREB1 and non-transgenic wheat varieties.
PRJNA341486 National Research Council of Canada small RNA and RNA profiling in Triticum turgidum and Triticum aestivum - Illumina HiSeq 2500 Identification of key genes for wax production.
PRJNA326902 Agriculture and Agri-Food Canada Deep sequencing of wheat sRNA transcriptome reveals distinct temporal expression pattern of miRNAs in response to heat, light and UV Deep sequencing of wheat sRNA transcriptome reveals distinct temporal expression pattern of miRNAs in response to heat, light and UV Illumina HiSeq 2000 Understanding of plant adaptation to abiotic stresses has implications in plant breeding, especially in the context of climate change. MicroRNAs (miRNAs) and short interfering RNAs play a crucial role in gene regulation. Here, wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). A total of 72 small RNA libraries were sequenced on the Illumina platform generating ~524 million reads corresponding to ~129 million distinct tags from which 232 conserved miRNAs were identified. The expression levels of 1, 2 and 79 miRNAs were affected by ultraviolet radiations, continuous light and heat, respectively. Approximately 55% of the differentially expressed miRNAs were downregulated at 0 and 1 DAT including miR398, miR528 and miR156 that control mRNAs involved in activation of signal transduction pathways and flowering. Other putative targets included histone variants and methyltransferases. These results suggest a temporal miRNA-guided post-transcriptional regulation that enables wheat to respond to abiotic stresses, particularly heat. Designing novel wheat breeding strategies such as regulatory gene-based marker assisted selection depends on accurate identification of stress induced miRNAs. Overall design: Wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). A total of 72 small RNA libraries were sequenced on the Illumina platform.
PRJNA309881 Henan Agricultural University Triticum aestivum Transcriptome or Gene expression Identification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro Culture Illumina HiSeq 2000 wheat callus miRNA.
PRJNA309061 Agricultural Biotechnology Center Triticum aestivum Raw sequence reads Comparison of small RNA next-generation sequencing with and without isolation of small RNA fraction Illumina HiScanSQ The aim of the present study was to compare data obtained by sequencing small libraries prepared from isolated small RNA fraction and from total RNA.
PRJNA284172 NRCPB Triticum aestivum cultivar:HD-2967 Transcriptome or Gene expression Molecular Characterization of GS2 and Fd-GOGAT Homeologues and Their Biased Response to Nitrogen Stress in Bread Wheat (Triticum aestivum L.) Illumina HiSeq 2000 microRNAs from root and shoot tissues of wheat. Submitted by: Subodh K. Sinha and P. K. Mandal.
PRJNA275100 Institute of Crop Science Expression profiling of small RNAs in developing wheat grains Small RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat Grains Illumina HiSeq 2000 We aimed to identify differentially expressed miRNAs during wheat grain development by using high-throughput sequencing approach. Four small RNA libraries were constructed from wheat grains collected at 7, 14, 21 and 28 days post anthesis (DPA). A total of 165 known miRNAs and 37 novel miRNAs were identified in four small RNA libraries. Moreover, a miRNA-like long hairpin locus was first identified to produce 21~22-nt phased siRNAs. A comparison of the miRNAomes revealed that 55 miRNA families were differentially expressed during the grain development. Overall design: Examination of 4 different small RNA expression profilings in the 4 developmental stages of wheat grains.
PRJNA270216 Henan agricultural university Triticum aestivum Transcriptome or Gene expression Identification and comparative analysis of differentially expressed miRNAs in leaves of two wheat (Triticum aestivum L.) genotypes during dehydration stress Illumina HiSeq 2000 To identify those miRNAs involved in dehydration stress tolerance.
PRJNA266709 BIRLA INSTITUTE OF TECHNOLOGY Triticum aestivum cultivar:HD2329 Uncovering leaf rust responsive miRNAs in wheat (Triticum aestivum L.) using high-throughput sequencing and prediction of their targets through degradome analysis Illumina MiSeq The major threat to wheat production in past decades has been the rust epidemics. The present study was targeted towards understanding of the mechanism and functional characterization of miRNAs of wheat in response to leaf rust ingression. With the improvement in methods to explore the transcriptome, in recent years there have been great advances in identifying and understanding non-coding RNAs. Recent studies indicate involvements of miRNAs in plant development, growth and responses to biotic and abiotic stresses, adaptive responses, metabolism, and signal transduction. Therefore, Illumina's deep sequencing technology was used for transcriptome-wide identification of miRNAs and their expression profiling in response to leaf rust infection using mock and pathogen inoculated resistant (HD2329+Lr24) and susceptible (HD2329) near-isogenic wheat plants.
PRJNA242577 University of Electronic Science and Technology of China Triticum aestivum Identification of Novel miRNAs and miRNA Expression Profiling in Wheat Hybrid Necrosis Illumina HiSeq 2000 Identification of Novel miRNAs and miRNA Expression Profiling in Wheat Hybrid Necrosis.
PRJNA241290 China Agricultural University Triticum aestivum strain:Chinese spring Transcriptome or Gene expression Whole-genome discovery of miRNAs and their targets in wheat (Triticum aestivum L.) Illumina Genome Analyzer IIx To identify as much wheat miRNAs and their targets
PRJNA171754 Indian Agricultural Research Institute Triticum aestivum strain:HD2985, HD2329 Transcriptome or Gene expression Identification of Putative RuBisCo Activase (TaRca1)—The Catalytic Chaperone Regulating Carbon Assimilatory Pathway in Wheat (Triticum aestivum) under the Heat Stress Illumina HiSeq 2000 The study was conducted in order to find out the differential change in the transcript of tolerant and susceptible wheat cultivar under heat stress and to decipher the mechanism of thermotolerance in wheat by identifying novel genes and transcription factors involved in the pathways. Wheat cultivar HD2985 (thermotolerant) and HD2329 (thermosusceptible) were exposed to heat stress of 42 degree for 4h at pollination stage and samples were collected from both control and heat shock treated plants for further characterization.
PRJNA719092 The University of Adelaide Small RNA, transcriptome and degradome analysis of the transgenerational heat stress response network in durum wheat (durum wheat) Small RNA, Transcriptome and Degradome Analysis of the Transgenerational Heat Stress Response Network in Durum Wheat Illumina NovaSeq 6000 Heat stress is a major limiting factor for grain yield and grain quality in wheat production. In crops, abiotic stresses have transgenerational effects and the mechanistic basis of stress memory is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Genotype-dependent response patterns to parental and progeny heat stress were found for the leaf physiological traits, harvest components, and grain quality traits measured. Parental heat stress had positive influence on the offspring under re-occurring stress for traits like chlorophyll content, grain weight, grain number and grain total starch content. Integrated sequencing analysis of the small RNAome, mRNA transcriptome, and mRNA degradome provided the first description of the molecular networks mediating heat stress adaption under transgenerational influence. The expression profile of 1771 microRNAs (733 being novel) and 66,559 genes was provided, with differentially expressed microRNAs and genes identified subject to the progeny treatment, parental treatment and tissue type factors. Gene Ontology and KEGG pathway annotation of stress responsive microRNAs-mRNA modules provided further information on their functional roles in biological processes like hormone homeostasis, signal transduction, and protein stabilization. Our results provide new sights on the molecular basis of transgenerational heat stress adaptation, which can be used for improving thermos-tolerance in breeding. Overall design: Analysis of small RNA transcriptome, mRNA transcriptome and mRNA degradome in eight biological pools (four treatment groups by two tissue types).
PRJNA706013 The University of Adelaide Nitrogen starvation-responsive microRNAs are affected by transgenerational stress in durum wheat seedlings (durum wheat) Nitrogen Starvation-Responsive MicroRNAs Are Affected by Transgenerational Stress in Durum Wheat Seedlings Illumina NovaSeq 6000 Stress events have transgenerational effects that influence plant growth in the subsequent generation. In Mediterranean regions, water-deficit and heat (WH) stress is a frequent issue that negatively affects crop yield and quality. Nitrogen (N) is an essential plant macronutrient and often a yield-limiting factor for crops. Here, the response of durum wheat seedlings to N starvation under the transgenerational effects of WH stress were investigated in two genotypes. Both genotypes showed significant reduction in seedling height, leaf number, shoot and root weight (fresh and dry), primary root length and chlorophyll content under N starvation stress. However, in the WH stress-tolerant genotype, the reduction rate of most traits were lower in progeny from the stressed parents than progeny from the control parents. Small RNA sequencing identified 1,534 microRNAs in different treatment groups. Differentially expressed microRNAs (DEMs) were characterized subject to N starvation, parental stress and genotype factors, with their target genes identified in silico. GO and KEGG enrichment analyses revealed the biological functions associated with DEM-target modules in stress adaptation processes, which could contribute to the phenotypic differences observed in two genotypes. The study provides the first evidence of the transgenerational effects of WH stress on N starvation response in durum wheat. Overall design: Analysis of small RNA transcriptome in eight biological pools (two genotypes by two parental treatment by two progeny treatment).
PRJNA680308 The University of Adelaide Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat Illumina NovaSeq 6000 Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on the parental plants during reproduction could have transgenerational effects on the progenies. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in the progenies. Parental water-deficit stress had positive impacts on the progenies in certain traits like harvest index and protein content in given genotype. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter- generational stress. Integrated analysis based on the three omics revealed the significant biological interactions between stress-responsive miRNA and targets, with possible contributions towards transgenerational stress tolerance via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained on the molecular level indicate that key miRNA-mRNA modules are potential.
PRJNA644573 The University of Adelaide Multi-omics analysis of small RNA, transcriptome and degradome in T. turgidum - regulatory networks of grain development and abiotic stress response (durum wheat) Multi-Omics Analysis of Small RNA, Transcriptome, and Degradome in T. turgidum-Regulatory Networks of Grain Development and Abiotic Stress Response Illumina NovaSeq 6000 Crop reproduction is highly sensitive to water-deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (T. turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome and degradome in T. turgidum developing grains, under single and combined water-deficit and heat stress. We identified 690 microRNAs (miRNAs), with 84 being novel, from 118 sRNA libraries. Complete profiles of differentially expressed miRNA (DEMs) specific to genotypes, stress types and different reproductive time-points are provided. The first degradome-seq report for developing durum grains discovered a significant number of new target genes regulated by miRNAs post-transcriptionally. Transcriptome-seq profiled 53,146 T. turgidum genes, with differentially expressed genes (DEGs) enriched in functional categories such as nutrient metabolism, cellular differentiation, transport, reproductive development and hormone transduction pathways. miRNA-mRNA networks that affect grain characteristics such as starch synthesis and protein metabolism were constructed, based on integrated analysis of the three omics. This study provides a substantial amount of novel information on the post-transcriptional networks in T. turgidum grains, which will facilitate innovations for breeding programs aiming to improve crop resilience and grain quality. Overall design: Analysis of small RNA transcriptome in 118 samples (2 genotypes x4 treatments x5 time-points x3 biological replicates); Analysis of mRNA transcriptome in eight libraries (2 genotypes x4 treatments x1 time-point x1 pool of 3 biological replicates); Analysis of mRNA degradome in eight libraries (2 genotypes x4 treatments x1 time-point x1 pool of 3 biological replicates).
PRJNA641098 The University of Adelaide ntegrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat (durum wheat) Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat Illumina NovaSeq 6000 Water-deficit and heat stress negatively impact crop production. Mechanisms underlying the response of durum wheat to such stresses are not well understood. With the new durum wheat genome assembly, we conducted the first multi-omics analysis with next-generation sequencing, providing a comprehensive description of the durum wheat small RNAome (sRNAome), mRNA transcriptome, and degradome. Single and combined water-deficit and heat stress were applied to stress-tolerant and -sensitive Australian genotypes to study their response at multiple time-points during reproduction. Analysis of 120 sRNA libraries identified 523 microRNAs (miRNAs), of which 55 were novel. Differentially expressed miRNAs (DEMs) were identified that had significantly altered expression subject to stress type, genotype, and time-point. Transcriptome sequencing identified 49,436 genes, with differentially expressed genes (DEGs) linked to processes associated with hormone homeostasis, photosynthesis, and signaling. With the first durum wheat degradome report, over 100,000 transcript target sites were characterized, and new miRNA-mRNA regulatory pairs were discovered. Integrated omics analysis identified key miRNA-mRNA modules (particularly, novel pairs of miRNAs and transcription factors) with antagonistic regulatory patterns subject to different stresses. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis revealed significant roles in plant growth and stress adaptation. Our research provides novel and fundamental knowledge, at the whole-genome level, for transcriptional and post-transcriptional stress regulation in durum wheat. Overall design: Analysis of small RNA transcriptome in 120 samples (2 genotypes x 4 treatments x 5 time-points x 3 biological replicates); Analysis of mRNA transcriptome in eight libraries (2 genotypes x 4 treatments x 1 time-point x 1 pool of 3 biological replicates); Analysis of mRNA degradome in eight libraries (2 genotypes x 4 treatments x 1 time-point x 1 pool of 3 biological replicates).
PRJNA600522 The University of Adelaide Transgenerational effects of water-deficit and heat stress on germination and seedling vigour - new insights from durum wheat microRNAs (durum wheat) Transgenerational Effects of Water-Deficit and Heat Stress on Germination and Seedling Vigour-New Insights from Durum Wheat microRNAs Illumina HiSeq 2500 Water deficiency and heat stress can severely limit crop production and quality. Stress imposed on the parents during reproduction could have transgenerational effects on their progeny. Seeds with different origins can vary significantly in germination time-course and early growth. Here, we investigated how water-deficit and heat stress on parental durum wheat plants affected seedling establishment of the subsequent generation. One stress-tolerant and one stress-sensitive Australian durum genotype were used. Seeds were collected from parents with or without exposure to stress during reproduction. Generally stress on the previous generation negatively affected seed germination and seedling vigour, but to a lesser extent in the tolerant variety. Small RNA sequencing utilising the new durum genome assembly has revealed significant differences in microRNA (miRNA) expression in the two genotypes. A bioinformatics approach was used to identify multiple miRNA targets which have critical molecular functions in stress adaptation and plant development and could therefore contribute to the phenotypic differences observed. Our data provides the first confirmation of the transgenerational effects of reproductive-stage stress on germination and seedling establishment in durum wheat. New insights gained on the epigenetic level indicate that durum miRNAs could be key factors in optimising seed vigour for superior breeding germplasm and/or varieties. Overall design: Examine the seedling miRNA population from two seed source of two genotypes.
PRJNA473404 Institute of Agricultural Biology and Biotechnolog Svevo Durum Wheat Durum wheat genome highlights past domestication signatures and future improvement targets Illumina HiSeq 2000 A reference genome sequence for Svevo durum wheat has been produced and annotated.
PRJNA310686 CNR - National Research Council Triticum turgidum subsp. durum transcriptome sequences Durum wheat miRNAs in response to nitrogen starvation at the grain filling stage Illumina Genome Analyzer II Discovery and analysis of microRNAs in durum wheat.
PRJNA285254 The University of Adelaide Genome-wide identification of differentially expressed microRNAs in leaves and the developing head of four durum genotypes during water deficit stress Genome-Wide Identification of MicroRNAs in Leaves and the Developing Head of Four Durum Genotypes during Water Deficit Stress Illumina HiSeq 2500 MicroRNAs are endogenous small noncoding RNAs that play critical roles in plant abiotic stress responses. The interaction between miRNA-mRNA targets and their regulatory pathways in response to water deficit stress has been investigated in many plant species. However, the miRNA transcriptome of durum wheat (Triticum turgidum L. ssp. durum) is poorly characterised, with little known about miRNA functions related to water deficit stress. Yield loss in durum wheat can be exacerbated due to minimal rainfall in the early reproductive stages of development during Spring in Australia. This study describes genotypic differences in the miRNAome between water deficit tolerant/sensitive durum, using flag leaf and developing head tissue, and more specifically identifies miRNAs associated with water deficit stress. Results: Small RNA libraries (96 in total) were constructed from flag leaf and developing head tissues of four durum genotypes (Tamaroi, Yawa, EGA Bellaroi, Tjilkuri), with or without water deficit stress. Illumina sequencing and subsequent analysis detected 110 conserved miRNAs and 159 novel candidate miRNA hairpins. Statistical analysis of the abundance of sequencing reads revealed 66 conserved miRNAs and five novel miRNA hairpins showing differential expression under water deficit stress. During stress, several conserved and novel miRNAs showed unambiguous inverted regulatory profiles between the durum genotypes studied. Several miRNAs were also identified to have different abundance in the flag leaf compared to the developing head regardless of treatment. Predicted mRNA targets from four novel durum miRNAs were characterised using Gene Ontology (GO) which revealed functions common to stress responses and plant development. Conclusion: For the first time, we present a comprehensive study of the miRNA transcriptome of flag leaf and developing head tissues in different durum genotypes under water deficit stress. The identification of differentially expressed miRNAs provides molecular evidence that miRNAs are potential determinants of water stress tolerance in durum wheat. GO analysis of predicted targets contributes to the understanding of genotype-specific physiological responses leading to stress tolerance capacity. Further functional analysis of specific stress responsive miRNAs identified, and their interaction with mRNA targets is ongoing and will assist in developing future durum wheat varieties with enhanced water deficit stress tolerance. Overall design: A total of 96 small RNA libraries were analysed [4 durum genotypes * 2 tissue types * 2 treatment groups (control and water stress) * 6 biological replicates] in this study. Please see Methods, Liu et al.(2015)
PRJNA383463 Nanjing Agricultural University Asymmetrical changes in chromatin and small RNAs contribute to altered gene expression and endosperm development in resynthesized wheat allotetraploids Asymmetrical changes of gene expression, small RNAs and chromatin in two resynthesized wheat allotetraploids Illumina HiSeq 2000 We performed genome-wide analyses of mRNA and small RNA transcriptomes in the endosperm of two synthetic wheats SSAA and AADD that differ in seed fertility and size. Transcriptomes between the endosperm and root of AADD were also analyzed to test a developmental role. Immunostaining experiments were performed to analyze the changes of histone H3K9me2 modifications in the chromosomes of two wheat allotetraploids and their progenitors.
PRJNA915324 Institute of Genetics and Developmental Biology Wheat RNA sequencing New insights on the evolution of nucleolar dominance in newly resynthesized hexaploid wheat Triticum Zhukovskyi Illumina HiSeq 2500 RNA-seq was carried out using material from seedling betweenl AG x A hybridization F1 and AG x D hybridization F1 .
PRJDB4663 KOBE_U Deep sequencing of small RNAs in grass-clump dwarf phenotype of abnormal growth triploids in interspecific crosses between tetraploid wheat and Aegilops tauschii Global gene expression profiling related to temperature-sensitive growth abnormalities in interspecific crosses between tetraploid wheat and Aegilops tauschii Illumina HiSeq 2000 Triploid wheat hybrids between tetraploid wheat and Aegilops tauschii sometimes show abnormal growth phenotypes, and the growth abnormalities inhibit generation of wheat synthetic hexaploids. In type II necrosis, one of the growth abnormalities, necrotic cell death accompanied by marked growth repression occurs only under low temperature conditions. At normal temperature, the type II necrosis lines show grass-clump dwarfism with no necrotic symptoms, excess tillers, more severe dwarfism or delayed flowering. We compared small RNA expression profiles in crown tissues to characterize the temperature-dependent phenotypic plasticity. Some microRNAs, including miR156, were up-regulated, whereas the levels of transcripts of the miR156 target genes SPLs, known to inhibit tiller and branch number, were reduced in crown tissues of the grass-clump dwarf lines at the normal temperature. Dramatic alteration of gene expression profiles, including miRNA levels, in crown tissues is associated with the temperature-dependent phenotypic plasticity in type II necrosis/grass-clump dwarf wheat hybrids.
PRJNA742257 Inner Mongolia Agricultural University Agropyron mongolicum cultivar:Agropyron mongolicum keng Genome sequencing and assembly Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng Illumina HiSeq 2500 The seeds of Agropyron mongolicum Keng were collected from Salaqi Agropyron mongolicum Keng experimental base Inner Mongolia Agricultural University Inner Mongolia Autonomous Region China Select full seeds remove lemma disinfect them with sodium hypochlorite solution for 15 minutes then wash them with sterilized distilled water for 5 times put them into germination box and cultivate them in indoor incubator set the temperature at 24 degrees centigrade photoperiod for 16 hours supplement evaporation water every day until the seedlings grow to the stage of three leaves one heart The leaves of Agropyron mongolicum Keng treated with drought for 12 h 24 h 48 h 3 D 5 d 7 d and rewatered for 24 h were sampled The leaves without drought treatment were set as control and stored in liquid nitrogen in pre cooled cryopreservation tube it was quickly put into the pre freezing tube and stored in liquid nitrogen.
PRJNA431719 Inner Mongolia Agricultural University Agropyron mongolicum Keng Raw sequence reads Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress Illumina HiSeq 2500 Agropyron mongolicum Keng is a predominant perennial forage in the steppes of Inner Mongolian Plateau, China. It is known to be tolerant to drought, low temperature and salinity, and is also valuable resource in wheat improvement. However, the corresponding molecular mechanism underlying the adaptation to water shortage in A. mongolicum remains uncharacterized.In this study, the microRNA of the Agropyron mongolicum was identified under the conditions of control and drought treatment.This would provide important information for further gene expression and functional genomics studies, confirmed our results on the molecular mechanisms of drought response in A. mongolicum and useful resources for crop drought resistant breeding.
PRJNA541381 Northwest Agriculture & Forestry University sRNA sequencing of Psathyrostachys huashanica Genome-wide miRNA expression profiling in Psathyrostachys huashanica reveals insights into the resistance response to barley yellow dwarf virus-GAV infection Illumina HiSeq 2500 sRNA sequencing of different time points of Psathyrostachys huashanica infected with BYDV-GAV.

Detailed information on different bioprojects

Run Library Name LibrarySelection Ecotype Age Tissue Cultivar Sample Name geo_loc_name
SRR12708724ZN_3cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708725ZN_2cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708726ZN_1cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708727KL14S_4cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708728KL14S_2cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708729KL14S_1cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708730KL14N_4cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708731KL14N_3cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708732ZS_4cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708733ZS_3cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708734ZS_2cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708735ZS_1cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708736ZN_4cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708737KL14N_2cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining
SRR12708738KL14N_1cDNATibetanHulless BarleyOne yearleavesKunlun14 and Z1140Hordeum vulgare L. var. nudum Hook. f.China Xining

Run Library Name LibrarySelection Tissue Cultivar Sample Name
SRR1166427ControlPCREndospermPI 564601Sinai_eIF4E locus
SRR1166403ControlPCREndospermPI 542707Dryland_eIF4E
SRR1166399ControlPCREndospermPI 510559Peru_eIF4E
SRR1166398ControlPCREndospermPI 1862613Control_Japan_eIF4E
SRR1166397QIPCREndospermQasr Ibrim strainQI_eIF4E
SRR1166396QIPCREndospermQasr Ibrim strainQI_sukkula
SRR1166395QIPCREndospermQasr Ibrim strainQI_Mla
SRR1166394QIPCREndospermQasr Ibrim strainQI_Lr34
SRR1166393QIPCREndospermQasr Ibrim strainQI_AGL97
SRR1166379QI_LXPCREndospermQasr Ibrim strainQasr Ibrim barley, AGL97

Run Library Name LibrarySelection Ecotype Stage Tissue Sample Name geo_loc_name
SRR8797395FN_1RANDOM PCRQinghai-Tibet PlateauseedlingleafHTLChina: Tibet
SRR8797396FN_2RANDOM PCRQinghai-Tibet PlateauseedlingleafHTLChina: Tibet
SRR8797397ON_1RANDOM PCRQinghai-Tibet PlateauseedlingleafHTLChina: Tibet
SRR8797398ON_2RANDOM PCRQinghai-Tibet PlateauseedlingleafHTLChina: Tibet
SRR8797399HN_1RANDOM PCRQinghai-Tibet PlateauseedlingleafHTLChina: Tibet
SRR8797400HN_2RANDOM PCRQinghai-Tibet PlateauseedlingleafHTLChina: Tibet

Run Library Name LibrarySelection Age Tissue Cultivar Sample Name geo_loc_name Treatment Replicate
SRR8265663XZ16-S-Salt-2RT-PCRabout 25-day-oldShootTibetan wild barley accession XZ16XZ16-S-Salt-2China: Qinghai Tibet Plateau200mM NaClreplicate 2
SRR8265664XZ16-S-SaltRT-PCRabout 25-day-oldShootTibetan wild barley accession XZ16XZ16-S-Salt-1China: Qinghai Tibet Plateau200mM NaClreplicate 1
SRR8265665XZ16-R-ControlRT-PCRabout 25-day-oldRootTibetan wild barley accession XZ16XZ16-R-Control-1China: Qinghai Tibet Plateaucontrolreplicate 1
SRR8265667GP-R-Control-2RT-PCRabout 25-day-oldRootGolden PromiseGP-R-Control-2-controlreplicate 2
SRR8265668XZ16-R-Control-2RT-PCRabout 25-day-oldRootTibetan wild barley accession XZ16XZ16-R-Control-2China: Qinghai Tibet Plateaucontrolreplicate 2
SRR8265669GP-R-ControlRT-PCRabout 25-day-oldRootGolden PromiseGP-R-Control-1-controlreplicate 1
SRR8265670XZ16-R-Salt-2RT-PCRabout 25-day-oldRootTibetan wild barley accession XZ16XZ16-R-Salt-2China: Qinghai Tibet Plateau200mM NaClreplicate 2
SRR8265671GP-R-SaltRT-PCRabout 25-day-oldRootGolden PromiseGP-R-Salt-1-200mM NaClreplicate 1
SRR8265672GP-S-Control-2RT-PCRabout 25-day-oldShootGolden PromiseGP-S-Control-2-controlreplicate 2
SRR8265673XZ16-R-SaltRT-PCRabout 25-day-oldRootTibetan wild barley accession XZ16XZ16-R-Salt-1China: Qinghai Tibet Plateau200mM NaClreplicate 1
SRR8265674GP-S-ControlRT-PCRabout 25-day-oldShootGolden PromiseGP-S-Control-1-controlreplicate 1
SRR8265675GP-R-Salt-2RT-PCRabout 25-day-oldRootGolden PromiseGP-R-Salt-2-200mM NaClreplicate 2
SRR8265676GP-S-Salt-2RT-PCRabout 25-day-oldShootGolden PromiseGP-S-Salt-2-200mM NaClreplicate 2
SRR8265677GP-S-SaltRT-PCRabout 25-day-oldShootGolden PromiseGP-S-Salt-1-200mM NaClreplicate 1
SRR8265678XZ16-S-Control-2RT-PCRabout 25-day-oldShootTibetan wild barley accession XZ16XZ16-S-Control-2China: Qinghai Tibet Plateaucontrolreplicate 2
SRR8265679XZ16-S-ControlRT-PCRabout 25-day-oldShootTibetan wild barley accession XZ16XZ16-S-Control-1China: Qinghai Tibet Plateaucontrolreplicate 1

Run Library Name LibrarySelection Stage Tissue Cultivar Sample Name
SRR18652253BSMV-SDN1gs/+BYDV-1size fractionationseedlingleafGolden PromiseBSMV-SDN1gs/+BYDV-1
SRR18652254BSMV-EV/+BYDV-3size fractionationseedlingleafGolden PromiseBSMV-EV/+BYDV-3
SRR18652255BSMV-EV/+BYDV-2size fractionationseedlingleafGolden PromiseBSMV-EV/+BYDV-2
SRR18652256BSMV-EV/+BYDV-1size fractionationseedlingleafGolden PromiseBSMV-EV/+BYDV-1
SRR18652257BSMV-SDN1gs/-BYDV-3size fractionationseedlingleafGolden PromiseBSMV-SDN1gs/-BYDV-3
SRR18652258BSMV-SDN1gs/-BYDV-2size fractionationseedlingleafGolden PromiseBSMV-SDN1gs/-BYDV-2
SRR18652259BSMV-SDN1gs/-BYDV-1size fractionationseedlingleafGolden PromiseBSMV-SDN1gs/-BYDV-1
SRR18652260BSMV-EV/-BYDV-3size fractionationseedlingleafGolden PromiseBSMV-EV/-BYDV-3
SRR18652261BSMV-SDN1gs/+BYDV-3size fractionationseedlingleafGolden PromiseBSMV-SDN1gs/+BYDV-3
SRR18652262BSMV-SDN1gs/+BYDV-2size fractionationseedlingleafGolden PromiseBSMV-SDN1gs/+BYDV-2
SRR18652263BSMV-EV/-BYDV-2size fractionationseedlingleafGolden PromiseBSMV-EV/-BYDV-2
SRR18652264BSMV-EV/-BYDV-1size fractionationseedlingleafGolden PromiseBSMV-EV/-BYDV-1

Run Library Name LibrarySelection Ecotype Stage Tissue Cultivar Sample Name
SRR18651738SB_4_1size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_4_1
SRR18651739M_14_3size fractionationWTseedling stageRootGolden PromiseM_14_3
SRR18651740M_14_2size fractionationWTseedling stageRootGolden PromiseM_14_2
SRR18651741M_14_1size fractionationWTseedling stageRootGolden PromiseM_14_1
SRR18651742M_7_3size fractionationWTseedling stageRootGolden PromiseM_7_3
SRR18651743M_7_2size fractionationWTseedling stageRootGolden PromiseM_7_2
SRR18651744M_7_1size fractionationWTseedling stageRootGolden PromiseM_7_1
SRR18651745M_4_3size fractionationWTseedling stageRootGolden PromiseM_4_3
SRR18651746SB_14_3size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_14_3
SRR18651747SB_14_2size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_14_2
SRR18651748SB_14_1size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_14_1
SRR18651749SB_7_3size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_7_3
SRR18651750SB_7_2size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_7_2
SRR18651751SB_7_1size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_7_1
SRR18651752SB_4_3size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_4_3
SRR18651753SB_4_2size fractionationSnRK1-YFPseedling stageRootGolden PromiseSB_4_2
SRR18651754M_4_2size fractionationWTseedling stageRootGolden PromiseM_4_2
SRR18651755M_4_1size fractionationWTseedling stageRootGolden PromiseM_4_1

Run Library Name LibrarySelection Age Tissue Cultivar Sample Name
SRR18331513FCH7NV5BBXYRANDOM PCRTriple moundSAMMorexHVSAMTM
SRR18331514FCH7NV5BBXXRANDOM PCRDouble ridgeSAMMorexHvSAMDR

Run Library Name LibrarySelection Age Tissue Cultivar Sample Name geo_loc_name
SRR18094627RSB15248_10RANDOM10 daysleafMargretEVplusGermany: Aachen
SRR18094628RSB15248_9RANDOM11 daysleafMargretP40Germany: Aachen
SRR18094629RSB15248_8RANDOM11 daysleafMargretP40Germany: Aachen
SRR18094630RSB15248_7RANDOM11 daysleafMargretP40Germany: Aachen
SRR18094631RSB15248_6RANDOM11 daysleaf-HauGermany: Aachen
SRR18094632RSB15248_5RANDOM11 daysleaf-HauGermany: Aachen
SRR18094633RSB15248_4RANDOM11 daysleaf-HauGermany: Aachen
SRR18094634RSB15248_3RANDOM11 daysleafMargretEpiGermany: Aachen
SRR18094635RSB15248_18RANDOM11 daysleaf-MycGermany: Aachen
SRR18094636RSB15248_17RANDOM11 daysleaf-MycGermany: Aachen
SRR18094637RSB15248_16RANDOM11 daysleaf-MycGermany: Aachen
SRR18094638RSB15248_15RANDOM10 daysleafMargretEVplusGermany: Aachen
SRR18094639RSB15248_14RANDOM10 daysleafMargretEVminusGermany: Aachen
SRR18094640RSB15248_13RANDOM10 daysleafMargretEVminusGermany: Aachen
SRR18094641RSB15248_12RANDOM10 daysleafMargretEVminusGermany: Aachen
SRR18094642RSB15248_11RANDOM10 daysleafMargretEVplusGermany: Aachen
SRR18094643RSB15248_2RANDOM11 daysleafMargretEpiGermany: Aachen
SRR18094644RSB15248_1RANDOM11 daysleafMargretEpiGermany: Aachen

Run LibrarySelection Sample Name geo_loc_name
ERR4566424size fractionationS12Australia
ERR4566423size fractionationS2Australia
ERR4566422size fractionationS0Australia

Run LibrarySelection Tissue Cultivar Sample Name Treatment Replicate
SRR13397555size fractionationdry embryo + scutellumDamazyGSM5012470Rc - after seed regeneration; non-stored controlrep 1
SRR13397554size fractionationdry embryo + scutellumDamazyGSM5012469Rc - after seed regeneration; non-stored controlrep 3
SRR13397553size fractionationdry embryo + scutellumDamazyGSM5012469Rc - after seed regeneration; non-stored controlrep 2
SRR13397552size fractionationdry embryo + scutellumDamazyGSM5012467Rc - after seed regeneration; non-stored controlrep 1
SRR13397551size fractionationdry embryo + scutellumDamazyGSM5012466Lv - seeds after long-term storage 1972-2018 (germination 2%)rep 3
SRR13397550size fractionationdry embryo + scutellumDamazyGSM5012465Lv - seeds after long-term storage 1972-2018 (germination 2%)rep 2
SRR13397549size fractionationdry embryo + scutellumDamazyGSM5012464Lv - seeds after long-term storage 1972-2018 (germination 2%)rep 1
SRR13397548size fractionationdry embryo + scutellumDamazyGSM5012463Hv - seeds after long-term storage 1972-2018 (germination 86,7%)rep 3
SRR13397547size fractionationdry embryo + scutellumDamazyGSM5012462Hv - seeds after long-term storage 1972-2018 (germination 86,7%)rep 2
SRR13397546size fractionationdry embryo + scutellumDamazyGSM5012461Hv - seeds after long-term storage 1972-2018 (germination 86,7%)rep 1

Run LibrarySelection Time Tissue Cultivar Phenotype Sample Name Treatment
SRR13183018size fractionation7 daysshootXZ153low K tolerantXZ153low K
SRR13183017size fractionation7 daysshootXZ153low K tolerantXZ153low K
SRR13183016size fractionation7 daysshootXZ153low K tolerantXZ153low K
SRR13183015size fractionation7 daysshootXZ153low K tolerantXZ153control
SRR13183014size fractionation7 daysshootXZ153low K tolerantXZ153control
SRR13183013size fractionation7 daysshootXZ153low K tolerantXZ153control
SRR13183012size fractionation7 daysshootZD9low K sensitiveZD9low K
SRR13183011size fractionation7 daysshootZD9low K sensitiveZD9low K
SRR13183010size fractionation7 daysshootZD9low K sensitiveZD9low K
SRR13183009size fractionation7 daysshootZD9low K sensitiveZD9control
SRR13183008size fractionation7 daysshootZD9low K sensitiveZD9control
SRR13183007size fractionation7 daysshootZD9low K sensitiveZD9control
SRR13183006size fractionation2 daysshootXZ153low K tolerantXZ153low K
SRR13183005size fractionation2 daysshootXZ153low K tolerantXZ153low K
SRR13183004size fractionation2 daysshootXZ153low K tolerantXZ153low K
SRR13183003size fractionation2 daysshootXZ153low K tolerantXZ153control
SRR13183002size fractionation2 daysshootXZ153low K tolerantXZ153control
SRR13183001size fractionation2 daysshootXZ153low K tolerantXZ153control
SRR13183000size fractionation2 daysshootZD9low K sensitiveZD9low K
SRR13182999size fractionation2 daysshootZD9low K sensitiveZD9low K
SRR13182998size fractionation2 daysshootZD9low K sensitiveZD9low K
SRR13182997size fractionation2 daysshootZD9low K sensitiveZD9control
SRR13182996size fractionation2 daysshootZD9low K sensitiveZD9control
SRR13182995size fractionation2 daysshootZD9low K sensitiveZD9control

Run Library Name Age Tissue Cultivar Sample Name geo_loc_name Replicate
SRR11881520Hvu-1.0-1_M_sRNA-seq1.0 mm antherAntherMorexHvu-1.0-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881531Hvu-0.8-3_M_sRNA-seq0.8 mm antherAntherMorexHvu-0.8-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881552Hvu-0.8-1_M_sRNA-seq0.8 mm antherAntherMorexHvu-0.8-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881563Hvu-0.6-3_M_sRNA-seq0.6 mm antherAntherMorexHvu-0.6-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881574Hvu-0.6-2_M_sRNA-seq0.6 mm antherAntherMorexHvu-0.6-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881585Hvu-0.6-1_M_sRNA-seq0.6 mm antherAntherMorexHvu-0.6-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881596Hvu-0.4-4_M_sRNA-seq0.4 mm antherAntherMorexHvu-0.4-4_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881605Hvu-1.8-3_GP_sRNA-seq1.8 mm antherAntherGolden PromiseHvu-1.8-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881606Hvu-1.8-2_GP_sRNA-seq1.8 mm antherAntherGolden PromiseHvu-1.8-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881607Hvu-0.4-2_M_sRNA-seq0.4 mm antherAntherMorexHvu-0.4-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881608Hvu-1.8-1_GP_sRNA-seq1.8 mm antherAntherGolden PromiseHvu-1.8-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881609Hvu-1.4-3_GP_sRNA-seq1.4 mm antherAntherGolden PromiseHvu-1.4-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881610Hvu-1.4-2_GP_sRNA-seq1.4 mm antherAntherGolden PromiseHvu-1.4-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881611Hvu-1.4-1_GP_sRNA-seq1.4 mm antherAntherGolden PromiseHvu-1.4-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881612Hvu-1.0-4_GP_sRNA-seq1.0 mm antherAntherGolden PromiseHvu-1.0-4_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881613Hvu-0.8-2_M_sRNA-seq0.8 mm antherAntherMorexHvu-0.8-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881614Hvu-0.2-2_M_sRNA-seq0.2 mm antherAntherMorexHvu-0.2-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881615Hvu-0.2-1_M_sRNA-seq0.2 mm antherAntherMorexHvu-0.2-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881616Hvu-1.0-2_GP_sRNA-seq1.0 mm antherAntherGolden PromiseHvu-1.0-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881617Hvu-1.0-1_GP_sRNA-seq1.0 mm antherAntherGolden PromiseHvu-1.0-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881618Hvu-0.8-3_GP_sRNA-seq0.8 mm antherAntherGolden PromiseHvu-0.8-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881619Hvu-0.8-2_GP_sRNA-seq0.8 mm antherAntherGolden PromiseHvu-0.8-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881620Hvu-0.8-1_GP_sRNA-seq0.8 mm antherAntherGolden PromiseHvu-0.8-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881621Hvu-0.4-1_M_sRNA-seq0.4 mm antherAntherMorexHvu-0.4-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881622Hvu-0.6-3_GP_sRNA-seq0.6 mm antherAntherGolden PromiseHvu-0.6-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881623Hvu-0.6-2_GP_sRNA-seq0.6 mm antherAntherGolden PromiseHvu-0.6-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881624Hvu-0.6-1_GP_sRNA-seq0.6 mm antherAntherGolden PromiseHvu-0.6-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881625Hvu-0.4-3_GP_sRNA-seq0.4 mm antherAntherGolden PromiseHvu-0.4-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881626Hvu-0.4-2_GP_sRNA-seq0.4 mm antherAntherGolden PromiseHvu-0.4-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881627Hvu-0.4-1_GP_sRNA-seq0.4 mm antherAntherGolden PromiseHvu-0.4-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881628Hvu-0.2-3_GP_sRNA-seq0.2 mm antherAntherGolden PromiseHvu-0.2-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881629Hvu-0.2-2_GP_sRNA-seq0.2 mm antherAntherGolden PromiseHvu-0.2-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881630Hvu-0.2-1_GP_sRNA-seq0.2 mm antherAntherGolden PromiseHvu-0.2-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881631Hvu-1.8-3_M_sRNA-seq1.8 mm antherAntherMorexHvu-1.8-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881632Hvu-0.2-3_M_sRNA-seq0.2 mm antherAntherMorexHvu-0.2-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881633Hvu-1.8-2_M_sRNA-seq1.8 mm antherAntherMorexHvu-1.8-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881634Hvu-1.8-1_M_sRNA-seq1.8 mm antherAntherMorexHvu-1.8-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881635Hvu-1.4-4_M_sRNA-seq1.4 mm antherAntherMorexHvu-1.4-4_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881636Hvu-1.4-3_M_sRNA-seq1.4 mm antherAntherMorexHvu-1.4-3_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881637Hvu-1.4-1_M_sRNA-seq1.4 mm antherAntherMorexHvu-1.4-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881638Hvu-1.0-3_M_sRNA-seq1.0 mm antherAntherMorexHvu-1.0-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881639Hvu-1.0-2_M_sRNA-seq1.0 mm antherAntherMorexHvu-1.0-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881537Tae-1.8-3_F_sRNA-seq1.8 mm antherAntherFielderTae-1.8-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881538Tae-1.8-2_F_sRNA-seq1.8 mm antherAntherFielderTae-1.8-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881539Tae-1.8-1_F_sRNA-seq1.8 mm antherAntherFielderTae-1.8-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881540Tae-1.4-4_F_sRNA-seq1.4 mm antherAntherFielderTae-1.4-4_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881541Tae-1.4-3_F_sRNA-seq1.4 mm antherAntherFielderTae-1.4-3_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881542Tae-1.4-1_F_sRNA-seq1.0 mm antherAntherFielderTae-1.4-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881543Tae-1.0-3_F_sRNA-seq1.0 mm antherAntherFielderTae-1.0-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881544Tae-1.0-2_F_sRNA-seq1.0 mm antherAntherFielderTae-1.0-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881545Tae-1.0-1_F_sRNA-seq1.0 mm antherAntherFielderTae-1.0-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881546Tae-0.8-3_F_sRNA-seq0.8 mm antherAntherFielderTae-0.8-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881547Tae-0.8-2_F_sRNA-seq0.8 mm antherAntherFielderTae-0.8-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881548Tae-0.8-1_F_sRNA-seq0.8 mm antherAntherFielderTae-0.8-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881549Tae-0.6-3_F_sRNA-seq0.6 mm antherAntherFielderTae-0.6-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881550Tae-0.6-2_F_sRNA-seq0.6 mm antherAntherFielderTae-0.6-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881551Tae-0.6-1_F_sRNA-seq0.6 mm antherAntherFielderTae-0.6-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881553Tae-0.4-4_F_sRNA-seq0.4 mm antherAntherFielderTae-0.4-4_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881554Tae-0.4-2_F_sRNA-seq0.4 mm antherAntherFielderTae-0.4-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881555Tae-0.4-1_F_sRNA-seq0.4 mm antherAntherFielderTae-0.4-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881556Tae-0.2-3_F_sRNA-seq0.2 mm antherAntherFielderTae-0.2-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881557Tae-0.2-2_F_sRNA-seq0.2 mm antherAntherFielderTae-0.2-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881558Tae-0.2-1_F_sRNA-seq0.2 mm antherAntherFielderTae-0.2-1_F_sRNA-seqUSA: St. Louisbiological replicate 1

Run Age Tissue Cultivar Sample Name geo_loc_name Replicate
SRR11833940Day 5microsporeGobernadoraDay5_rep2Canada: Quebecreplicate 2
SRR11833941Day 5microsporeGobernadoraDay5_rep.1Canada: Quebecreplicate 1
SRR11833942Day 2microsporeGobernadoraDay2_rep4Canada: Quebecreplicate 4
SRR11833943Day 2microsporeGobernadoraDay2_rep3Canada: Quebecreplicate 3
SRR11833944Day 2microsporeGobernadoraDay2_rep2Canada: Quebecreplicate 2
SRR11833945Day 2microsporeGobernadoraDay2_rep1Canada: Quebecreplicate 1
SRR11833946Day 0microsporeGobernadoraDay0_rep4Canada: Quebecreplicate 4
SRR11833947Day 0microsporeGobernadoraDay0_rep3Canada: Quebecreplicate 3
SRR11833951Day 5microsporeGobernadoraDay5_rep4Canada: Quebecreplicate 4
SRR11833952Day 5microsporeGobernadoraDay5_rep3Canada: Quebecreplicate 3
SRR11833953Day 0microsporeGobernadoraDay0_rep2Canada: Quebecreplicate 2
SRR11833954Day 0microsporeGobernadoraDay0_rep1Canada: Quebecreplicate 1

Run LibrarySelection Tissue Cultivar Sample Name Replicate
SRR11153177size fractionationrootGolden PromiseGSM4333095rep3
SRR11153176size fractionationshootGolden PromiseGSM4333094rep3
SRR11153175size fractionationrootGolden PromiseGSM4333093rep3
SRR11153174size fractionationshootGolden PromiseGSM4333092rep3
SRR11153173size fractionationrootGolden PromiseGSM4333091rep2
SRR11153172size fractionationshootGolden PromiseGSM4333090rep2
SRR11153171size fractionationrootGolden PromiseGSM4333089rep2
SRR11153170size fractionationshootGolden PromiseGSM4333088rep2
SRR11153169size fractionationrootGolden PromiseGSM4333087rep1
SRR11153168size fractionationrootGolden PromiseGSM4333086rep1
SRR11153167size fractionationshootGolden PromiseGSM4333085rep1
SRR11153166size fractionationshootGolden PromiseGSM4333084rep1

Run LibrarySelection Tissue Sample Name
SRR11098940size fractionationrootGSM4318310
SRR11098939size fractionationrootGSM4318309
SRR11098938size fractionationrootGSM4318308
SRR11098937size fractionationrootGSM4318307
SRR11098936size fractionationrootGSM4318306
SRR11098935size fractionationrootGSM4318305
SRR11098934size fractionationshootGSM4318304
SRR11098933size fractionationshootGSM4318303
SRR11098932size fractionationshootGSM4318302
SRR11098931size fractionationshootGSM4318301
SRR11098930size fractionationshootGSM4318300
SRR11098929size fractionationshootGSM4318299

Run LibrarySelection Tissue Sample Name Treatment
SRR11098909size fractionationrootGSM4318279low Pi condition
SRR11098908size fractionationshootGSM4318278low Pi condition

Run LibrarySelection Genotype Timepoint Tissue Sample Name Replicate
SRR7363414size fractionationm19028 (mla6, Rar3, bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204658Rep 3
SRR7363413size fractionationm19028 (mla6, Rar3, bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204657Rep 2
SRR7363412size fractionationm19028 (mla6, Rar3, bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204656Rep 1
SRR7363411size fractionationm19028 (mla6, Rar3, bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204655Rep 3
SRR7363410size fractionationm19028 (mla6, Rar3, bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204654Rep 2
SRR7363409size fractionationm19028 (mla6, Rar3, bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204653Rep 1
SRR7363408size fractionationm19028 (mla6, Rar3, bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204652Rep 3
SRR7363407size fractionationm19028 (mla6, Rar3, bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204651Rep 2
SRR7363406size fractionationm19028 (mla6, Rar3, bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204650Rep 1
SRR7363405size fractionationm19028 (mla6, Rar3, bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204649Rep 3
SRR7363404size fractionationm19028 (mla6, Rar3, bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204648Rep 2
SRR7363403size fractionationm19028 (mla6, Rar3, bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204647Rep 1
SRR7363402size fractionationm19028 (mla6, Rar3, bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204646Rep 3
SRR7363401size fractionationm19028 (mla6, Rar3, bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204645Rep 2
SRR7363400size fractionationm19028 (mla6, Rar3, bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204644Rep 1
SRR7363399size fractionationm19028 (mla6, Rar3, bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204643Rep 3
SRR7363398size fractionationm19028 (mla6, Rar3, bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204642Rep 2
SRR7363397size fractionationm19028 (mla6, Rar3, bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204641Rep 1
SRR7363396size fractionationm19089 (Mla6, Rar3, bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204640Rep 3
SRR7363395size fractionationm19089 (Mla6, Rar3, bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204639Rep 2
SRR7363394size fractionationm19089 (Mla6, Rar3, bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204638Rep 1
SRR7363393size fractionationm19089 (Mla6, Rar3, bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204637Rep 3
SRR7363392size fractionationm19089 (Mla6, Rar3, bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204636Rep 2
SRR7363391size fractionationm19089 (Mla6, Rar3, bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204635Rep 1
SRR7363390size fractionationm19089 (Mla6, Rar3, bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204634Rep 3
SRR7363389size fractionationm19089 (Mla6, Rar3, bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204633Rep 2
SRR7363388size fractionationm19089 (Mla6, Rar3, bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204632Rep 1
SRR7363387size fractionationm19089 (Mla6, Rar3, bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204631Rep 3
SRR7363386size fractionationm19089 (Mla6, Rar3, bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204630Rep 2
SRR7363385size fractionationm19089 (Mla6, Rar3, bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204629Rep 1
SRR7363384size fractionationm19089 (Mla6, Rar3, bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204628Rep 3
SRR7363383size fractionationm19089 (Mla6, Rar3, bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204627Rep 2
SRR7363382size fractionationm19089 (Mla6, Rar3, bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204626Rep 1
SRR7363381size fractionationm19089 (Mla6, Rar3, bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204625Rep 3
SRR7363380size fractionationm19089 (Mla6, Rar3, bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204624Rep 2
SRR7363379size fractionationm19089 (Mla6, Rar3, bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204623Rep 1
SRR7363378size fractionationm11526 (Mla6, rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204622Rep 3
SRR7363377size fractionationm11526 (Mla6, rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204621Rep 2
SRR7363376size fractionationm11526 (Mla6, rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204620Rep 1
SRR7363375size fractionationm11526 (Mla6, rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204619Rep 3
SRR7363374size fractionationm11526 (Mla6, rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204618Rep 2
SRR7363373size fractionationm11526 (Mla6, rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204617Rep 1
SRR7363372size fractionationm11526 (Mla6, rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204616Rep 3
SRR7363371size fractionationm11526 (Mla6, rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204615Rep 2
SRR7363370size fractionationm11526 (Mla6, rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204614Rep 1
SRR7363369size fractionationm11526 (Mla6, rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204613Rep 3
SRR7363368size fractionationm11526 (Mla6, rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204612Rep 2
SRR7363367size fractionationm11526 (Mla6, rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204611Rep 1
SRR7363366size fractionationm11526 (Mla6, rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204610Rep 3
SRR7363365size fractionationm11526 (Mla6, rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204609Rep 2
SRR7363364size fractionationm11526 (Mla6, rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204608Rep 1
SRR7363363size fractionationm11526 (Mla6, rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204607Rep 3
SRR7363362size fractionationm11526 (Mla6, rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204606Rep 2
SRR7363361size fractionationm11526 (Mla6, rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204605Rep 1
SRR7363360size fractionationm18982 (mla6, Rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204604Rep 3
SRR7363359size fractionationm18982 (mla6, Rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204603Rep 2
SRR7363358size fractionationm18982 (mla6, Rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204602Rep 1
SRR7363357size fractionationm18982 (mla6, Rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204601Rep 3
SRR7363356size fractionationm18982 (mla6, Rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204600Rep 2
SRR7363355size fractionationm18982 (mla6, Rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204599Rep 1
SRR7363354size fractionationm18982 (mla6, Rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204598Rep 3
SRR7363353size fractionationm18982 (mla6, Rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204597Rep 2
SRR7363352size fractionationm18982 (mla6, Rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204596Rep 1
SRR7363351size fractionationm18982 (mla6, Rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204595Rep 3
SRR7363350size fractionationm18982 (mla6, Rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204594Rep 2
SRR7363349size fractionationm18982 (mla6, Rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204593Rep 1
SRR7363348size fractionationm18982 (mla6, Rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204592Rep 3
SRR7363347size fractionationm18982 (mla6, Rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204591Rep 2
SRR7363346size fractionationm18982 (mla6, Rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204590Rep 1
SRR7363345size fractionationm18982 (mla6, Rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204589Rep 3
SRR7363344size fractionationm18982 (mla6, Rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204588Rep 2
SRR7363343size fractionationm18982 (mla6, Rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204587Rep 1
SRR7363342size fractionationCI 16151 (Mla6, Rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204586Rep 3
SRR7363341size fractionationCI 16151 (Mla6, Rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204585Rep 2
SRR7363340size fractionationCI 16151 (Mla6, Rar3, Bln1)48 HAIBarley infected first leaf (PO:0007094)GSM3204584Rep 1
SRR7363339size fractionationCI 16151 (Mla6, Rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204583Rep 3
SRR7363338size fractionationCI 16151 (Mla6, Rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204582Rep 2
SRR7363337size fractionationCI 16151 (Mla6, Rar3, Bln1)32 HAIBarley infected first leaf (PO:0007094)GSM3204581Rep 1
SRR7363336size fractionationCI 16151 (Mla6, Rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204580Rep 3
SRR7363335size fractionationCI 16151 (Mla6, Rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204579Rep 2
SRR7363334size fractionationCI 16151 (Mla6, Rar3, Bln1)24 HAIBarley infected first leaf (PO:0007094)GSM3204578Rep 1
SRR7363333size fractionationCI 16151 (Mla6, Rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204577Rep 3
SRR7363332size fractionationCI 16151 (Mla6, Rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204576Rep 2
SRR7363331size fractionationCI 16151 (Mla6, Rar3, Bln1)20 HAIBarley infected first leaf (PO:0007094)GSM3204575Rep 1
SRR7363330size fractionationCI 16151 (Mla6, Rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204574Rep 3
SRR7363329size fractionationCI 16151 (Mla6, Rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204573Rep 2
SRR7363328size fractionationCI 16151 (Mla6, Rar3, Bln1)16 HAIBarley infected first leaf (PO:0007094)GSM3204572Rep 1
SRR7363327size fractionationCI 16151 (Mla6, Rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204571Rep 3
SRR7363326size fractionationCI 16151 (Mla6, Rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204570Rep 2
SRR7363325size fractionationCI 16151 (Mla6, Rar3, Bln1)0 HAIBarley infected first leaf (PO:0007094)GSM3204569Rep 1

Run Library Name LibrarySelection Age Tissue Cultivar Sample Name geo_loc_name
SRR8698934s2-2size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698935s2-3size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698936spike-3size fractionation68 daysspikeRolapsRNA-spikePoland
SRR8698937s2-1size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698938spike-1size fractionation68 daysspikeRolapsRNA-spikePoland
SRR8698939spike-2size fractionation68 daysspikeRolapsRNA-spikePoland
SRR86989406w-2size fractionation6 weekswhole plantRolapsRNA-6wPoland
SRR86989416w-3size fractionation6 weekswhole plantRolapsRNA-6wPoland
SRR8698942k5-1size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698943k5-2size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698944k5-3size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698945assize fractionationflag leafwhole plantRolappare-droughPoland
SRR8698946aksize fractionationflag leafwhole plantRolappare-droughPoland
SRR8698947hksize fractionationmissingspikeRolappare-spikePoland
SRR8698948k2-1size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698949k2-2size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR86989503w-3size fractionation3 weekswhole plantRolapsRNA-3wPoland
SRR86989516w-1size fractionation6 weekswhole plantRolapsRNA-6wPoland
SRR86989523w-1size fractionation3 weekswhole plantRolapsRNA-3wPoland
SRR86989533w-2size fractionation3 weekswhole plantRolapsRNA-3wPoland
SRR86989542w-2size fractionation2 weekswhole plantRolapsRNA-2wPoland
SRR86989552w-3size fractionation2 weekswhole plantRolapsRNA-2wPoland
SRR86989561w-3size fractionation1 weekwhole plantRolapsRNA-1wPoland
SRR86989572w-1size fractionation2 weekswhole plantRolapsRNA-2wPoland
SRR86989581w-1size fractionation1 weekwhole plantRolapsRNA-1wPoland
SRR86989591w-2size fractionation1 weekwhole plantRolapsRNA-1wPoland
SRR8698960s5-3size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698961s5-2size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698962s5-1size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698963k3-3size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698964k3-2size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698965k3-1size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698966s3-3size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698967s3-2size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698968s3-1size fractionationflag leafwhole plantRolapsRNA-droughtPoland
SRR8698969k2-3size fractionationflag leafwhole plantRolapsRNA-droughtPoland

Run Library Name LibrarySelection Age Tissue Cultivar Sample Name Replicate
SRR7687211Root_WB-1_Cd1cDNA22 daysrootWB-1Root_C_Cd1WB-1_Cd_root_replication 1
SRR7687212Root_WB-1_Cd2cDNA22 daysrootWB-1Root_C_Cd2WB-1_Cd_root_replication 2
SRR7687213Root_GP_Cd1cDNA22 daysrootGolden PromiseRoot_A_Cd1GP_Cd_root_replication 1
SRR7687214Root_GP_Cd2cDNA22 daysrootGolden PromiseRoot_A_Cd2GP_Cd_root_replication 2
SRR7687215Root_WB-1_CK1cDNA22 daysrootWB-1Root_C_CK1WB-1_CK_root_replication 1
SRR7687216Root_WB-1_CK2cDNA22 daysrootWB-1Root_C_CK2WB-1_CK_root_replication 2
SRR7687217Root_GP_CK1cDNA22 daysrootGolden PromiseRoot_A_CK1GP_CK_root_replication 1
SRR7687218Root_GP_CK2cDNA22 daysrootGolden PromiseRoot_A_CK2GP_CK_root_replication 2
SRR7687219Shoot_WB-1_Cd1cDNA22 daysshootWB-1Shoot_C_Cd1WB-1_Cd_shoot_replication 1
SRR7687220Shoot_WB-1_Cd2cDNA22 daysshootWB-1Shoot_C_Cd2WB-1_Cd_shoot_replication 2
SRR7687221Shoot_GP_Cd1cDNA22 daysshootGolden PromiseShoot_A_Cd1GP_Cd_shoot_replication 1
SRR7687222Shoot_GP_Cd2cDNA22 daysshootGolden PromiseShoot_A_Cd2GP_Cd_shoot_replication 2
SRR7687223Shoot_WB-1_CK1cDNA22 daysshootWB-1Shoot_C_CK1WB-1_CK_shoot_replication 1
SRR7687224Shoot_WB-1_CK2cDNA22 daysshootWB-1Shoot_C_CK2WB-1_CK_shoot_replication 2
SRR7687225Shoot_GP_CK1cDNA22 daysshootGolden PromiseShoot_A_CK1GP_CK_shoot_replication 1
SRR7687226Shoot_GP_CK2cDNA22 daysshootGolden PromiseShoot_A_CK2GP_CK_shoot_replication 2

Run Library Name LibrarySelection Ecotype Age Tissue Cultivar Sample Name Replicate
SRR7543962XZ29-Al1cDNAAI tolerant20 daysroot-XZ29-Al1XZ29 Al replicate 1
SRR7543963XZ29-Al2cDNAAI tolerant20 daysroot-XZ29-Al2XZ29 Al replicate 2
SRR7543964XZ29 control1cDNAAI tolerant20 daysroot-XZ29 control1XZ29 control replicate 1
SRR7543965XZ29 control2cDNAAI tolerant20 daysroot-XZ29 control2XZ29 control replicate 2
SRR7543966Golden Promise-Al1cDNAAI sensitive20 daysrootGolden PromiseGolden Promise-Al1GP Al replicate 1
SRR7543967Golden Promise-Al2cDNAAI sensitive20 daysrootGolden PromiseGolden Promise-Al2GP Al replicate 2
SRR7543968Golden Promise control1cDNAAI sensitive20 daysrootGolden PromiseGolden Promise control1GP control replicate 1
SRR7543969Golden Promise control2cDNAAI sensitive20 daysrootGolden PromiseGolden Promise control2GP control replicate 2

Run Library Name LibrarySelection Cultivar Sample Name geo_loc_name
DRR107734Barley Small RNAsize fractionationMorexDRS051843Japan:Tsukuba

Run Library Name LibrarySelection Stage Tissue Cultivar Sample Name geo_loc_name
SRR5643510sRNA-seq_embryo-10DPAsize fractionationEmbryo, ten days post anthesisEmbryoGolden PromiseEmbryo-10DPAChina
SRR5643511sRNA-seq_seed-5DAGsize fractionationSeeds, five days after germinationSeedsGolden PromiseSeed-5DAGChina
SRR5643512sRNA-seq_seed-1DAGsize fractionationSeeds, one day after germinationSeedsGolden PromiseSeed-1DAGChina

Run Library Name LibrarySelection stage Tissue Cultivar Sample Name geo_loc_name Replicate
SRR5054866SUB2126437size fractionation8 Days Post AnthesisgrainClipersRNA_seq_grain_8_DPA_replicate2China:Northwest A&F University,Yangling, Shaanxireplicate2
SRR5054862SUB2126440size fractionation18 Days Post AnthesisgrainClipersRNA_seq_grain_18_DPA_replicate1China:Northwest A&F University,Yangling, Shaanxireplicate1
SRR5054861SUB2126433size fractionation3 Days Post AnthesisgrainClipersRNA_seq_grain_3_DPA_replicate1China:Northwest A&F University,Yangling, Shaanxireplicate1
SRR5054860SUB2126441size fractionation18 Days Post AnthesisgrainClipersRNA_seq_grain_18_DPA_replicate2China:Northwest A&F University,Yangling, Shaanxireplicate2
SRR5054859SUB2126436size fractionation8 Days Post AnthesisgrainClipersRNA_seq_grain_8_DPA_replicate1China:Northwest A&F University,Yangling, Shaanxireplicate1
SRR5054858SUB2126439size fractionation13 Days Post AnthesisgrainClipersRNA_seq_grain_13_DPA_replicate2China:Northwest A&F University,Yangling, Shaanxireplicate2
SRR5054853SUB2126438size fractionation13 Days Post AnthesisgrainClipersRNA_seq_grain_13_DPA_replicate1China:Northwest A&F University,Yangling, Shaanxireplicate1
SRR5054852SUB2126435size fractionation3 Days Post AnthesisgrainClipersRNA_seq_grain_3_DPA_replicate2China:Northwest A&F University,Yangling, Shaanxireplicate2

Run Library Name LibrarySelection Stage Tissue Cultivar Sample Name geo_loc_name Treatment
SRR2481856barley_8h_tsize fractionationseedlingall plantMorexbarley_8h_tChinasalinity stress
SRR2481855barley_8h_cksize fractionationseedlingall plantMorexbarley_8h_ckChinacontrol
SRR2481854barley_3h_tsize fractionationseedlingall plantMorexbarley_3h_tChinasalinity stress
SRR2481853barley_3h_cksize fractionationseedlingall plantMorexbarley_3h_ckChinacontrol
SRR2481852barley_27h_tsize fractionationseedlingall plantMorexbarley_27h_tChinasalinity stress
SRR2481851barley_sRNA barley_27h_cksize fractionationseedlingall plantMorexbarley_27h_ckChinacontrol

Run LibrarySelection Library Name Age Tissue Cultivar Sample Name geo_loc_name Replicate
SRR24282674size fractionationI-0214 dayswhole leafMcNair 701Infected_02USA: Pullmanrep2
SRR24282675size fractionationI-0114 dayswhole leafMcNair 701Infected_01USA: Pullmanrep1
SRR24282676size fractionationU-0314 dayswhole leafMcNair 701Uninfected_03USA: Pullmanrep3
SRR24282677size fractionationU-0214 dayswhole leafMcNair 701Uninfected_02USA: Pullmanrep2
SRR24282678size fractionationU-0114 dayswhole leafMcNair 701Uninfected_01USA: Pullmanrep1

Run LibrarySelection Library Name Ecotype Stage Tissue Cultivar Sample Name geo_loc_name
SRR23262625cDNATaSRootTurkeyTilleringRootYuregir-89TaSRootTurkey: Kastamonu
SRR23262626cDNATaCRootTurkeyTilleringRootYuregir-89TaCRootTurkey: Kastamonu
SRR23262629cDNATaSLeafTurkeyTilleringLeafYuregir-89TaSLeafTurkey: Kastamonu
SRR23262630cDNATaCLeafTurkeyTilleringLeafYuregir-89TaCLeafTurkey: Kastamonu
SRR23262621cDNATtSRootTurkeyTilleringRootKiziltan-91TtSRootTurkey: Kastamonu
SRR23262622cDNATtCRootTurkeyTilleringRootKiziltan-91TtCRootTurkey: Kastamonu
SRR23262623cDNATtSLeafTurkeyTilleringLeafKiziltan-91TtSLeafTurkey: Kastamonu
SRR23262624cDNATtCLeafTurkeyTilleringLeafKiziltan-91TtCLeafTurkey: Kastamonu
SRR23262619cDNATmSLeafTurkeyTilleringLeafSiyezTmSLeafTurkey: Kastamonu
SRR23262620cDNATmCLeafTurkeyTilleringLeafSiyezTmCLeafTurkey: Kastamonu
SRR23262627cDNATmSRootTurkeyTilleringRootSiyezTmSRootTurkey: Kastamonu
SRR23262628cDNATmCRootTurkeyTilleringRootSiyezTmCRootTurkey: Kastamonu

Run LibrarySelection Library Name Ecotype Age Tissue Cultivar Sample Name geo_loc_name Treatment
SRR22989766PCR(-Fe) SHOOTNABI MOHALI2 weeksSHOOTC306(-Fe) SHOOTIndiaFe Deficient
SRR22989767PCR(+Fe) SHOOTNABI MOHALI2 weeksSHOOTC306(+Fe) SHOOTIndiaFe sufficient
SRR22989768PCR(-Fe) ROOTNABI MOHALI2 weeksROOTC306(-Fe) ROOTIndiaFe Deficient
SRR22989769PCR(+Fe) ROOTNABI MOHALI2 weeksROOTC306(+Fe) ROOTIndiaFe sufficient

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name Treatment
SRR22103146RT-PCRWheat_L263_S1035DPAdeveloping seedsWaitoubaiWheat_L263_10Normal temperature
SRR22103147RT-PCRWheat_L263_S0928DPAdeveloping seedsWaitoubaiWheat_L263_09High temperature
SRR22103148RT-PCRWheat_L263_S0828DPAdeveloping seedsWaitoubaiWheat_L263_08High temperature
SRR22103149RT-PCRWheat_L263_S0728DPAdeveloping seedsWaitoubaiWheat_L263_07High temperature
SRR22103150RT-PCRWheat_L263_S0628DPAdeveloping seedsWaitoubaiWheat_L263_06Normal temperature
SRR22103151RT-PCRWheat_L263_S0528DPAdeveloping seedsWaitoubaiWheat_L263_05Normal temperature
SRR22103152RT-PCRWheat_L263_S0428DPAdeveloping seedsWaitoubaiWheat_L263_04Normal temperature
SRR22103163RT-PCRWheat_L263_S0321DPAdeveloping seedsWaitoubaiWheat_L263_03Normal temperature
SRR22103169RT-PCRWheat_L263_S1535DPAdeveloping seedsWaitoubaiWheat_L263_15High temperature
SRR22103170RT-PCRWheat_L263_S1435DPAdeveloping seedsWaitoubaiWheat_L263_14High temperature
SRR22103171RT-PCRWheat_L263_S1335DPAdeveloping seedsWaitoubaiWheat_L263_13High temperature
SRR22103172RT-PCRWheat_L263_S1235DPAdeveloping seedsWaitoubaiWheat_L263_12Normal temperature
SRR22103173RT-PCRWheat_L263_S1135DPAdeveloping seedsWaitoubaiWheat_L263_11Normal temperature
SRR22103174RT-PCRWheat_L263_S0221DPAdeveloping seedsWaitoubaiWheat_L263_02Normal temperature
SRR22103175RT-PCRWheat_L263_S0121DPAdeveloping seedsWaitoubaiWheat_L263_01Normal temperature

Run LibrarySelection Library Name Ecotype Age Tissue Cultivar Sample Name geo_loc_name
SRR21439292Oligo-dTDAC10_2korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439293Oligo-dTDAC10_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439294Oligo-dTDAT10_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439295Oligo-dTDAC6_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439296Oligo-dTDAT6_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Replicate
SRR21050554othersmallTFL-1-1heading dateovaryDong xuan 3smallTFL-1-1Chinareplicate=biological replicate 10
SRR21050555othersmallCK-3-3heading dateovaryDong xuan 3smallCK-3-3Chinareplicate=biological replicate 9
SRR21050556othersmallCK-3-2heading dateovaryDong xuan 3smallCK-3-2Chinareplicate=biological replicate 8
SRR21050557othersmallCK-3-1heading dateovaryDong xuan 3smallCK-3-1Chinareplicate=biological replicate 7
SRR21050558othersmallCK-2-3heading dateovaryDong xuan 3smallCK-2-3Chinareplicate=biological replicate 6
SRR21050559othersmallCK-2-2heading dateovaryDong xuan 3smallCK-2-2Chinareplicate=biological replicate 5
SRR21050560othersmallCK-2-1heading dateovaryDong xuan 3smallCK-2-1Chinareplicate=biological replicate 4
SRR21050561othersmallCK-1-3heading dateovaryDong xuan 3smallCK-1-3Chinareplicate=biological replicate 3
SRR21050562othersmallTFL-3-3heading dateovaryDong xuan 3smallTFL-3-3Chinareplicate=biological replicate 18
SRR21050563othersmallTFL-3-2heading dateovaryDong xuan 3smallTFL-3-2Chinareplicate=biological replicate 17
SRR21050564othersmallTFL-3-1heading dateovaryDong xuan 3smallTFL-3-1Chinareplicate=biological replicate 16
SRR21050565othersmallTFL-2-3heading dateovaryDong xuan 3smallTFL-2-3Chinareplicate=biological replicate 15
SRR21050566othersmallTFL-2-2heading dateovaryDong xuan 3smallTFL-2-2Chinareplicate=biological replicate 14
SRR21050567othersmallTFL-2-1heading dateovaryDong xuan 3smallTFL-2-1Chinareplicate=biological replicate 13
SRR21050568othersmallTFL-1-3heading dateovaryDong xuan 3smallTFL-1-3Chinareplicate=biological replicate 12
SRR21050569othersmallTFL-1-2heading dateovaryDong xuan 3smallTFL-1-2Chinareplicate=biological replicate 11
SRR21050570othersmallCK-1-2heading dateovaryDong xuan 3smallCK-1-2Chinareplicate=biological replicate 2
SRR21050571othersmallCK-1-1heading dateovaryDong xuan 3smallCK-1-1Chinareplicate=biological replicate 1

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name Treatment
DRR299372cDNALeaf rust Susceptible- Mock inoculationTwo-leaf stageleafHD2329LSMpathogen Infection
DRR299371cDNAStem rust resistant- Infection treatmentTwo-leaf stageleafHD2329+Sr36SRIMock
DRR299374cDNALeaf rust Resistant- Mock inoculationTwo-leaf stageleafHD2329 +Lr45SAMD00325718Mock
DRR299373cDNALeaf rust Susceptible- Infection treatedTwo-leaf stageleafHD2329SAMD00325717pathogen Infection
DRR299375cDNALeaf rust Resistant-Infection treatedTwo-leaf stageleafHD2329+Lr45SAMD00325719pathogen Infection
DRR299370cDNAStem rust Resistant- Mock inoculationTwo-leaf stageleafHD2329 +Sr36SAMD00325714Mock
DRR299369cDNAStem rust susceptible- Infection treatmentTwo-leaf stageleafHD2329SAMD00325713pathogen Infection
DRR299368cDNAStem rust susceptible- Mock inoculationTwo-leaf stageleafHD2329SAMD00325712Mock

Run Library Name Age Tissue Cultivar Sample Name geo_loc_name Treatment Replicate
SRR2094581Infected Louise 342 daysflag leafLouiseInfected LouiseUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094580Infected Louise 242 daysflag leafLouiseInfected LouiseUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094579Infected Louise 142 daysflag leafLouiseInfected LouiseUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094591Uninfected Louise 342 daysflag leafLouiseUninfected LouiseUSA: Washingtonmock inoculated with talcum powder-
SRR2094590Uninfected Louise 242 daysflag leafLouiseUninfected LouiseUSA: Washingtonmock inoculated with talcum powder-
SRR2094588Uninfected Louise 142 daysflag leafLouiseUninfected LouiseUSA: Washingtonmock inoculated with talcum powder-
SRR2094566Infected Penawawa 342 daysflag leafPenawawaInfected PenawawaUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094565Infected Penawawa 242 daysflag leafPenawawaInfected PenawawaUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094492Infected Penawawa 142 daysflag leafPenawawaInfected PenawawaUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094586Uninfected Penawawa 342 daysflag leafPenawawaUninfected PenawawaUSA: Washingtonmock inoculated with talcum powder-
SRR2094585Uninfected Penawawa 242 daysflag leafPenawawaUninfected PenawawaUSA: Washingtonmock inoculated with talcum powder-
SRR2094584Uninfected Penawawa 142 daysflag leafPenawawaUninfected PenawawaUSA: Washingtonmock inoculated with talcum powder-
SRR19441421I4_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI4_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 4
SRR19441422I3_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI3_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 3
SRR19441423I2_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI2_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 2
SRR19441424I1_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI1_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 1
SRR19441425U4_AvS_sRNA22 dayswhole shootAvocet SusceptibleU4_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 4
SRR19441426U3_AvS_sRNA22 dayswhole shootAvocet SusceptibleU3_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 3
SRR19441427U2_AvS_sRNA22 dayswhole shootAvocet SusceptibleU2_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 2
SRR19441428U1_AvS_sRNA22 dayswhole shootAvocet SusceptibleU1_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 1

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Treatment Replicate
SRR19238050size fractionationDT-T-1seedling stagerootZM13DT-T-1China:jinzhongdroughtbiological replicate 1
SRR19238051size fractionationDT-CK-3seedling stagerootZM13DT-CK-3China:jinzhongnormalbiological replicate 3
SRR19238052size fractionationDT-CK-2seedling stagerootZM13DT-CK-2China:jinzhongnormalbiological replicate 2
SRR19238053size fractionationDT-CK-1seedling stagerootZM13DT-CK-1China:jinzhongnormalbiological replicate 1
SRR19238054size fractionationDS-T-3seedling stagerootJM38DS-T-3China:jinzhongdroughtbiological replicate 3
SRR19238055size fractionationDS-T-2seedling stagerootJM38DS-T-2China:jinzhongdroughtbiological replicate 2
SRR19238056size fractionationDS-T-1seedling stagerootJM38DS-T-1China:jinzhongdroughtbiological replicate 1
SRR19238057size fractionationDS-CK-3seedling stagerootJM38DS-CK-3China:jinzhongnormalbiological replicate 3
SRR19238058size fractionationDT-T-3seedling stagerootZM13DT-T-3China:jinzhongdroughtbiological replicate 3
SRR19238059size fractionationDT-T-2seedling stagerootZM13DT-T-2China:jinzhongdroughtbiological replicate 2
SRR19238060size fractionationDS-CK-2seedling stagerootJM38DS-CK-2China:jinzhongnormalbiological replicate 2
SRR19238061size fractionationDS-CK-1seedling stagerootJM38DS-CK-1China:jinzhongnormalbiological replicate 1

Run LibrarySelection Library Name Stage Tissue Sample Name geo_loc_name
SRR17712120PCRCG_6h_1Trileaf stageWhole seedlingsCG_6h_1China:shannxi
SRR17712121PCRCG_1h_3Trileaf stageWhole seedlingsCG_1h_3China:shannxi
SRR17712122PCRCG_1h_2Trileaf stageWhole seedlingsCG_1h_2China:shannxi
SRR17712123PCRCG_1h_1Trileaf stageWhole seedlingsCG_1h_1China:shannxi
SRR17712124PCRCG_12h_3Trileaf stageWhole seedlingsCG_12h_3China:shannxi
SRR17712125PCRCG_12h_2Trileaf stageWhole seedlingsCG_12h_2China:shannxi
SRR17712126PCRDD_6h_3Trileaf stageWhole seedlingsDD_6h_3China:shannxi
SRR17712127PCRDD_6h_2Trileaf stageWhole seedlingsDD_6h_2China:shannxi
SRR17712128PCRDD_6h_1Trileaf stageWhole seedlingsDD_6h_1China:shannxi
SRR17712129PCRDD_1h_3Trileaf stageWhole seedlingsDD_1h_3China:shannxi
SRR17712130PCRDD_1h_2Trileaf stageWhole seedlingsDD_1h_2China:shannxi
SRR17712131PCRDD_1h_1Trileaf stageWhole seedlingsDD_1h_1China:shannxi
SRR17712132PCRCG_12h_1Trileaf stageWhole seedlingsCG_12h_1China:shannxi
SRR17712133PCRDD_12h_3Trileaf stageWhole seedlingsDD_12h_3China:shannxi
SRR17712134PCRDD_12h_2Trileaf stageWhole seedlingsDD_12h_2China:shannxi
SRR17712135PCRDD_12h_1Trileaf stageWhole seedlingsDD_12h_1China:shannxi
SRR17712136PCRDD_0h_3Trileaf stageWhole seedlingsDD_0h_3China:shannxi
SRR17712137PCRDD_0h_2Trileaf stageWhole seedlingsDD_0h_2China:shannxi
SRR17712138PCRDD_0h_1Trileaf stageWhole seedlingsDD_0h_1China:shannxi
SRR17712139PCRDM_6h_3Trileaf stageWhole seedlingsDM_6h_3China:shannxi
SRR17712140PCRDM_6h_2Trileaf stageWhole seedlingsDM_6h_2China:shannxi
SRR17712141PCRDM_6h_1Trileaf stageWhole seedlingsDM_6h_1China:shannxi
SRR17712142PCRDM_1h_3Trileaf stageWhole seedlingsDM_1h_3China:shannxi
SRR17712143PCRCG_0h_3Trileaf stageWhole seedlingsCG_0h_3China:shannxi
SRR17712144PCRDM_1h_2Trileaf stageWhole seedlingsDM_1h_2China:shannxi
SRR17712145PCRDM_1h_1Trileaf stageWhole seedlingsDM_1h_1China:shannxi
SRR17712146PCRDM_12h_3Trileaf stageWhole seedlingsDM_12h_3China:shannxi
SRR17712147PCRDM_12h_2Trileaf stageWhole seedlingsDM_12h_2China:shannxi
SRR17712148PCRDM_12h_1Trileaf stageWhole seedlingsDM_12h_1China:shannxi
SRR17712149PCRDM_0h_3Trileaf stageWhole seedlingsDM_0h_3China:shannxi
SRR17712150PCRDM_0h_2Trileaf stageWhole seedlingsDM_0h_2China:shannxi
SRR17712151PCRDM_0h_1Trileaf stageWhole seedlingsDM_0h_1China:shannxi
SRR17712152PCRCG_6h_3Trileaf stageWhole seedlingsCG_6h_3China:shannxi
SRR17712153PCRCG_6h_2Trileaf stageWhole seedlingsCG_6h_2China:shannxi
SRR17712154PCRCG_0h_2Trileaf stageWhole seedlingsCG_0h_2China:shannxi
SRR17712155PCRCG_0h_1Trileaf stageWhole seedlingsCG_0h_1China:shannxi

Run LibrarySelection Library Name Tissue Cultivar Sample Name Treatment Replicate
SRR17316475size fractionationGSM5750485leavesAkteurGSM5750485Non inoculatedrep4
SRR17316476size fractionationGSM5750484leavesAkteurGSM5750484Non inoculatedrep3
SRR17316477size fractionationGSM5750483leavesAkteurGSM5750483Non inoculatedrep2
SRR17316478size fractionationGSM5750482leavesAkteurGSM5750482Non inoculatedrep1
SRR17316479size fractionationGSM5750481leavesAkteurGSM5750481Non inoculatedrep4
SRR17316480size fractionationGSM5750480leavesAkteurGSM5750480Non inoculatedrep3
SRR17316481size fractionationGSM5750479leavesAkteurGSM5750479Non inoculatedrep2
SRR17316482size fractionationGSM5750478leavesAkteurGSM5750478Non inoculatedrep1
SRR17316483size fractionationGSM5750477leavesAkteurGSM5750477WDV infectedrep4
SRR17316484size fractionationGSM5750476leavesAkteurGSM5750476WDV infectedrep3
SRR17316485size fractionationGSM5750475leavesAkteurGSM5750475WDV infectedrep2
SRR17316486size fractionationGSM5750474leavesAkteurGSM5750474WDV infectedrep1
SRR17316487size fractionationGSM5750473leavesAkteurGSM5750473WDV infectedrep4
SRR17316488size fractionationGSM5750472leavesAkteurGSM5750472WDV infectedrep3
SRR17316489size fractionationGSM5750471leavesAkteurGSM5750471WDV infectedrep2
SRR17316490size fractionationGSM5750470leavesAkteurGSM5750470WDV infectedrep1
SRR17316491size fractionationGSM5750469leavesAkteurGSM5750469WDV infectedrep4
SRR17316492size fractionationGSM5750468leavesAkteurGSM5750468WDV infectedrep3
SRR17316493size fractionationGSM5750467leavesAkteurGSM5750467WDV infectedrep2
SRR17316494size fractionationGSM5750466leavesAkteurGSM5750466WDV infectedrep1
SRR17316495size fractionationGSM5750465leavesAkteurGSM5750465Non inoculatedrep4
SRR17316496size fractionationGSM5750464leavesAkteurGSM5750464Non inoculatedrep3
SRR17316497size fractionationGSM5750463leavesAkteurGSM5750463Non inoculatedrep2
SRR17316498size fractionationGSM5750462leavesAkteurGSM5750462Non inoculatedrep1
SRR17316499size fractionationGSM5750533leavesAkteurGSM5750533Non inoculatedrep4
SRR17316500size fractionationGSM5750532leavesSvitavaGSM5750532Non inoculatedrep3
SRR17316501size fractionationGSM5750531leavesSvitavaGSM5750531Non inoculatedrep2
SRR17316502size fractionationGSM5750530leavesSvitavaGSM5750530Non inoculatedrep1
SRR17316503size fractionationGSM5750529leavesSvitavaGSM5750529Non inoculatedrep4
SRR17316504size fractionationGSM5750528leavesSvitavaGSM5750528Non inoculatedrep3
SRR17316505size fractionationGSM5750527leavesSvitavaGSM5750527Non inoculatedrep2
SRR17316506size fractionationGSM5750526leavesSvitavaGSM5750526Non inoculatedrep1
SRR17316507size fractionationGSM5750525leavesSvitavaGSM5750525Non inoculatedrep4
SRR17316508size fractionationGSM5750524leavesSvitavaGSM5750524Non inoculatedrep3
SRR17316509size fractionationGSM5750523leavesSvitavaGSM5750523Non inoculatedrep2
SRR17316510size fractionationGSM5750522leavesSvitavaGSM5750522Non inoculatedrep1
SRR17316511size fractionationGSM5750521leavesSvitavaGSM5750521WDV infectedrep4
SRR17316512size fractionationGSM5750520leavesSvitavaGSM5750520WDV infectedrep3
SRR17316513size fractionationGSM5750519leavesSvitavaGSM5750519WDV infectedrep2
SRR17316514size fractionationGSM5750518leavesSvitavaGSM5750518WDV infectedrep1
SRR17316515size fractionationGSM5750517leavesSvitavaGSM5750517WDV infectedrep4
SRR17316516size fractionationGSM5750516leavesSvitavaGSM5750516WDV infectedrep3
SRR17316517size fractionationGSM5750515leavesSvitavaGSM5750515WDV infectedrep2
SRR17316518size fractionationGSM5750514leavesSvitavaGSM5750514WDV infectedrep1
SRR17316519size fractionationGSM5750513leavesSvitavaGSM5750513WDV infectedrep4
SRR17316520size fractionationGSM5750512leavesSvitavaGSM5750512WDV infectedrep3
SRR17316521size fractionationGSM5750511leavesSvitavaGSM5750511WDV infectedrep2
SRR17316522size fractionationGSM5750510leavesSvitavaGSM5750510WDV infectedrep1
SRR17316571size fractionationGSM5750509leavesFengyouGSM5750509Non inoculatedrep4
SRR17316572size fractionationGSM5750508leavesFengyouGSM5750508Non inoculatedrep3
SRR17316573size fractionationGSM5750507leavesFengyouGSM5750507Non inoculatedrep2
SRR17316574size fractionationGSM5750506leavesFengyouGSM5750506Non inoculatedrep1
SRR17316575size fractionationGSM5750505leavesFengyouGSM5750505WDV infectedrep4
SRR17316576size fractionationGSM5750504leavesFengyouGSM5750504WDV infectedrep3
SRR17316577size fractionationGSM5750503leavesFengyouGSM5750503WDV infectedrep2
SRR17316578size fractionationGSM5750502leavesFengyouGSM5750502WDV infectedrep1
SRR17316579size fractionationGSM5750501leavesFengyouGSM5750501WDV infectedrep4
SRR17316580size fractionationGSM5750500leavesFengyouGSM5750500WDV infectedrep3
SRR17316581size fractionationGSM5750499leavesFengyouGSM5750499WDV infectedrep2
SRR17316582size fractionationGSM5750498leavesFengyouGSM5750498WDV infectedrep1
SRR17316583size fractionationGSM5750497leavesFengyouGSM5750497WDV infectedrep4
SRR17316584size fractionationGSM5750496leavesFengyouGSM5750496WDV infectedrep3
SRR17316585size fractionationGSM5750495leavesFengyouGSM5750495WDV infectedrep2
SRR17316586size fractionationGSM5750494leavesFengyouGSM5750494WDV infectedrep1
SRR17316587size fractionationGSM5750493leavesFengyouGSM5750493Non inoculatedrep4
SRR17316588size fractionationGSM5750492leavesFengyouGSM5750492Non inoculatedrep3
SRR17316589size fractionationGSM5750491leavesFengyouGSM5750491Non inoculatedrep2
SRR17316590size fractionationGSM5750490leavesFengyouGSM5750490Non inoculatedrep1
SRR17316591size fractionationGSM5750489leavesFengyouGSM5750489Non inoculatedrep4
SRR17316592size fractionationGSM5750488leavesFengyouGSM5750488Non inoculatedrep3
SRR17316593size fractionationGSM5750487leavesFengyouGSM5750487Non inoculatedrep2
SRR17316594size fractionationGSM5750486leavesFengyouGSM5750486Non inoculatedrep1

Run LibrarySelection Age Tissue Cultivar Sample Name geo_loc_name
SRR13619124PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619125PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619126PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619127PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619128PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619129PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619130PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619131PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619132PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619133PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619134PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619135PCR10 daysroots and leaveswheatY174China:Anhui

Run LibrarySelection Library Name Ecotype Stage Tissue Sample Name geo_loc_name
SRR13222412PCRSMI-1Sumai3flowering phasespikelet and rachisSMChina
SRR13222413PCRSMM-3Sumai3flowering phasespikelet and rachisSMChina
SRR13222414PCRSMM-2Sumai3flowering phasespikelet and rachisSMChina
SRR13222415PCRSMM-1Sumai3flowering phasespikelet and rachisSMChina
SRR13222416PCRCSI-3Chinese springflowering phasespikelet and rachisCSChina
SRR13222417PCRCSI-2Chinese springflowering phasespikelet and rachisCSChina
SRR13222418PCRCSI-1Chinese springflowering phasespikelet and rachisCSChina
SRR13222419PCRCSM-3Chinese springflowering phasespikelet and rachisCSChina
SRR13222420PCRSMI-3Sumai3flowering phasespikelet and rachisSMChina
SRR13222421PCRSMI-2Sumai3flowering phasespikelet and rachisSMChina
SRR13222422PCRCSM-2Chinese springflowering phasespikelet and rachisCSChina
SRR13222423PCRCSM-1Chinese springflowering phasespikelet and rachisCSChina

Run LibrarySelection Stage Tissue Sample Name Treatment
SRR12419568size fractionationjointing stagecrown tissue colleected at jointing stageGSM4717211Non inoculated
SRR12419567size fractionationspring greenup stagecrown tissue colleected at spring greenup stageGSM4717210Non inoculated
SRR12419566size fractionationwinter dormancy stagecrown tissue colleected at winter dormancy stageGSM4717209Non inoculated
SRR12419565size fractionationthree leaf stagecrown tissue collected at the three leaf stageGSM4717208Non inoculated

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Treatment Replicate
SRR12102896size fractionationsRNA-seq_spikelet_rep3floweringSpikeletChinese spriingSpikelet_smallRNA_3China:Wuhansmall RNA-seq of wheatReplicate 3
SRR12102897size fractionationsRNA-seq_spikelet_rep2floweringSpikeletChinese spriingSpikelet_smallRNA_2China:Wuhansmall RNA-seq of wheatReplicate 2
SRR12102898size fractionationsRNA-seq_spikelet_rep1floweringSpikeletChinese spriingSpikelet_smallRNA_1China:Wuhansmall RNA-seq of wheatReplicate 1

Run LibrarySelection Library Name Age Tissue Cultivar Sample Name geo_loc_name Replicate
SRR10317096PCRSF1-1early uninucleateantherwheat TCMSSF1-1China:shannxibiological replicate1
SRR10317097PCRSS3-3binucleateantherwheat TCMSSS3-3China:shannxibiological replicate3
SRR10317098PCRSS3-2binucleateantherwheat TCMSSS3-2China:shannxibiological replicate2
SRR10317099PCRSS3-1binucleateantherwheat TCMSSS3-1China:shannxibiological replicate1
SRR10317100PCRSS2-3late uninucleateantherwheat TCMSSS2-3China:shannxibiological replicate3
SRR10317101PCRSS2-2late uninucleateantherwheat TCMSSS2-2China:shannxibiological replicate2
SRR10317102PCRSS2-1late uninucleateantherwheat TCMSSS2-1China:shannxibiological replicate1
SRR10317103PCRSS1-3early uninucleateantherwheat TCMSSS1-3China:shannxibiological replicate3
SRR10317104PCRSF3-3binucleateantherwheat TCMSSF3-3China:shannxibiological replicate3
SRR10317105PCRSF3-2binucleateantherwheat TCMSSF3-2China:shannxibiological replicate2
SRR10317106PCRSF3-1binucleateantherwheat TCMSSF3-1China:shannxibiological replicate1
SRR10317107PCRSF2-3late uninucleateantherwheat TCMSSF2-3China:shannxibiological replicate3
SRR10317108PCRSF2-2late uninucleateantherwheat TCMSSF2-2China:shannxibiological replicate2
SRR10317109PCRSF2-1late uninucleateantherwheat TCMSSF2-1China:shannxibiological replicate1
SRR10317110PCRSF1-3early uninucleateantherwheat TCMSSF1-3China:shannxibiological replicate3
SRR10317111PCRSF1-2early uninucleateantherwheat TCMSSF1-2China:shannxibiological replicate2
SRR10317112PCRSS1-2early uninucleateantherwheat TCMSSS1-2China:shannxibiological replicate2
SRR10317113PCRSS1-1early uninucleateantherwheat TCMSSS1-1China:shannxibiological replicate1

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Treatment
SRR10051481cDNAN0_17D_117_days_after_anthesisseedZhengmai119miRNA_LN17D1China:Zhengzhoudifferent nitrogen level
SRR10051482cDNAZ_17D_317_days_after_anthesisseedZhengmai119miRNA_HN17D3China:Zhengzhoudifferent nitrogen level
SRR10051483cDNAZ_17D_217_days_after_anthesisseedZhengmai119miRNA_HN17D2China:Zhengzhoudifferent nitrogen level
SRR10051484cDNAZ_17D_117_days_after_anthesisseedZhengmai119miRNA_HN17D1China:Zhengzhou-
SRR10051485cDNAN0_6D_36_days_after_anthesisseedZhengmai119miRNA_LN6D3China:Zhengzhou-
SRR10051486cDNAN0_6D_26_days_after_anthesisseedZhengmai119miRNA_LN6D2China:Zhengzhoudifferent nitrogen level
SRR10051487cDNAN0_6D_16_days_after_anthesisseedZhengmai119miRNA_LN6D1China:Zhengzhoudifferent nitrogen level
SRR10051488cDNAZ_6D_36_days_after_anthesisseedZhengmai119miRNA_HN6D3China:Zhengzhou-
SRR10051489cDNAN0_27D_327_days_after_anthesisseedZhengmai119miRNA_LN27D3China:Zhengzhoudifferent nitrogen level
SRR10051490cDNAN0_27D_227_days_after_anthesisseedZhengmai119miRNA_LN27D2China:Zhengzhoudifferent nitrogen level
SRR10051491cDNAN0_27D_127_days_after_anthesisseedZhengmai119miRNA_LN27D1China:Zhengzhoudifferent nitrogen level
SRR10051492cDNAZ_27D_327_days_after_anthesisseedZhengmai119miRNA_HN27D3China:Zhengzhoudifferent nitrogen level
SRR10051493cDNAZ_27D_227_days_after_anthesisseedZhengmai119miRNA_HN27D2China:Zhengzhoudifferent nitrogen level
SRR10051494cDNAZ_27D_127_days_after_anthesisseedZhengmai119miRNA_HN27D1China:Zhengzhoudifferent nitrogen level
SRR10051495cDNAN0_17D_317_days_after_anthesisseedZhengmai119miRNA_LN17D3China:Zhengzhoudifferent nitrogen level
SRR10051496cDNAN0_17D_217_days_after_anthesisseedZhengmai119miRNA_LN17D2China:Zhengzhoudifferent nitrogen level
SRR10051497cDNAZ_6D_26_days_after_anthesisseedZhengmai119miRNA_HN6D2China:Zhengzhou-
SRR10051498cDNAZ_6D_16_days_after_anthesisseedZhengmai119miRNA_HN6D1China:Zhengzhoudifferent nitrogen level

Run Library Name Age Tissue Cultivar Sample Name geo_loc_name Treatment
SRR9712609Tae Bgt 9622412 daysleafChinese SpringTae Bgt 96224Switzerland: ZurichBlumeria graminis infection
SRR9712610Tae Bgt 9420212 daysleafChinese SpringTae Bgt 94202Switzerland: ZurichBlumeria graminis infection
SRR9712611Tae Csuninf12 daysleafChinese SpringTae CsuninfSwitzerland: Zurich-
SRR9712612Tae Bgt JIW212 daysleafChinese SpringTae Bgt JIW2Switzerland: ZurichBlumeria graminis infection

Run LibrarySelection Stage Tissue Cultivar Sample Name geo_loc_name
SRR9696790RT-PCRlate double ridge stageyoung spikesguomai301WT-ys-S2aChina:Zhengzhou
SRR9696791RT-PCRlate double ridge stageyoung stem tipsguomai301WT-st-S1cChina:Zhengzhou
SRR9696792RT-PCRlate double ridge stageyoung stem tipsguomai301WT-st-S1bChina:Zhengzhou
SRR9696793RT-PCRlate double ridge stageyoung stem tipsguomai301WT-st-S1aChina:Zhengzhou
SRR9696794RT-PCRlate double ridge stageyoung stem tipsguomai301ptsd1-st-S3bChina:Zhengzhou
SRR9696795RT-PCRlate double ridge stageyoung stem tipsguomai301ptsd1-st-S3aChina:Zhengzhou
SRR9696796RT-PCRlate double ridge stageyoung spikesguomai301WT-ys-S2cChina:Zhengzhou
SRR9696797RT-PCRlate double ridge stageyoung spikesguomai301WT-ys-S2bChina:Zhengzhou
SRR9696798RT-PCRlate double ridge stageyoung spikesguomai301ptsd1-ys-S4aChina:Zhengzhou
SRR9696799RT-PCRlate double ridge stageyoung stem tipsguomai301ptsd1-st-S3cChina:Zhengzhou
SRR9696800RT-PCRlate double ridge stageyoung spikesguomai301ptsd1-ys-S4cChina:Zhengzhou
SRR9696801RT-PCRlate double ridge stageyoung spikesguomai301ptsd1-ys-S4bChina:Zhengzhou

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name
SRR9648458RT-PCRys_1_miRNAspike differentiation stage 5young spikesGuomai 301ys_1_miRNAChina:Zhengzhou
SRR9648459RT-PCRys_3_miRNAspike differentiation stage 5young spikesGuomai 301ys_3_miRNAChina:Zhengzhou
SRR9648460RT-PCRys_2_miRNAspike differentiation stage 5young spikesGuomai 301ys_2_miRNAChina:Zhengzhou
SRR9648461RT-PCRst_2_miRNAspike differentiation stage 5stem tipsGuomai 301st_2_miRNAChina:Zhengzhou
SRR9648462RT-PCRst_1_miRNAspike differentiation stage 5stem tipsGuomai 301st_1_miRNAChina:Zhengzhou
SRR9648463RT-PCRtp_1_miRNA3-leaf to 4-leaf stagetiller primordiaGuomai 301tp_1_miRNAChina:Zhengzhou
SRR9648464RT-PCRst_3_miRNAspike differentiation stage 5stem tipsGuomai 301st_3_miRNAChina:Zhengzhou
SRR9648465RT-PCRtp_3_miRNA3-leaf to 4-leaf stagetiller primordiaGuomai 301tp_3_miRNAChina:Zhengzhou
SRR9648466RT-PCRtp_2_miRNA3-leaf to 4-leaf stagetiller primordiaGuomai 301tp_2_miRNAChina:Zhengzhou

Run LibrarySelection Genotype Stage Tissue Cultivar Sample Name
SRR8467485size fractionationwild typejointing stageleafwinter wheat Shimai 22GSM3573290
SRR8467484size fractionationwild typespring greenup stageleafwinter wheat Shimai 22GSM3573289
SRR8467483size fractionationwild typewinter dormancy stageleafwinter wheat Shimai 22GSM3573288
SRR8467482size fractionationwild typewinter wheat collected at three leaf stageleafwinter wheat Shimai 22GSM3573287

Run LibrarySelection Library Name Sample Name
ERR2777611size fractionationL001_R1_001SAMEA4883093
ERR2777610size fractionationL001_R1_001SAMEA4883092
ERR2777609size fractionationL001_R1_001SAMEA4883091
ERR2777608size fractionationL001_R1_001SAMEA4883090
ERR2777607size fractionationL001_R1_001SAMEA4883089
ERR2777606size fractionationL001_R1_001SAMEA4883088
ERR2777605size fractionationL001_R1_001SAMEA4883087
ERR2777604size fractionationL001_R1_001SAMEA4883086
ERR2777603size fractionationL001_R1_001SAMEA4883085
ERR2777602size fractionationL001_R1_001SAMEA4883084
ERR2777601size fractionationL001_R1_001SAMEA4883083
ERR2777600size fractionationL001_R1_001SAMEA4883082
ERR2777599size fractionationL001_R1_001SAMEA4883081
ERR2777598size fractionationL001_R1_001SAMEA4883080
ERR2777597size fractionationL001_R1_001SAMEA4883079

Run Library Name Ecotype Stage Tissue Cultivar Sample Name geo_loc_name Lat_Lon Treatment Replicate
SRR8612151LK120hexaploidthree leaf stagerootKenong9204sample_6_deficient_K_120h_rootChina38.82 N 115.44 Edeficient-K treatment for 120hLK_120
SRR8612152LK48hexaploidthree leaf stagerootKenong9204sample_5_deficient_K_48h_rootChina38.82 N 115.44 Edeficient-K treatment for 48hLK_48
SRR8612153LK6hexaploidthree leaf stagerootKenong9204sample_2_deficient_K_6h_rootChina38.82 N 115.44 Edeficient-K treatment for 6hLK_6
SRR8612154CKhexaploidthree leaf stagerootKenong9204sample_1_deficient_K_0h_rootChina38.82 N 115.44 Edeficient-K treatment for 0hCK
SRR8612155LK24hexaploidthree leaf stagerootKenong9204sample_4_deficient_K_24h_rootChina38.82 N 115.44 Edeficient-K treatment for 24hLK_24
SRR8612156LK12hexaploidthree leaf stagerootKenong9204sample_3_deficient_K_12h_rootChina38.82 N 115.44 Edeficient-K treatment for 12hLK_12

Run LibrarySelection Library Name phenotype Stage Tissue Cultivar Sample Name geo_loc_name Treatment Replicate
SRR8432572RT-PCRWSB_sRNA-2Radult-plant stagespikeletsWangshuibaiWSB_sRNA-2China: JiangsuFusarium head blightWSB_small_RNA_library2
SRR8432573RT-PCRWSB_sRNA-1Radult-plant stagespikeletsWangshuibaiWSB_sRNA-1China: JiangsuFusarium head blightWSB_small_RNA_library1
SRR8432587RT-PCRSumai3_sRNA-2Radult-plant stagespikeletsSumai_3Sumai3_sRNA-2China: JiangsuFusarium head blightSumai3_small_RNA_library2
SRR8432590RT-PCRSumai3_sRNA-1Radult-plant stagespikeletsSumai_3Sumai3_sRNA-1China: JiangsuFusarium head blightSumai3_small_RNA_library1

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name
SRR8374804cDNAT3seedling stageleavesXiaoyan 6Triticum aestivum-leavesChina:shannxi
SRR8374805cDNAT2seedling stageleavesXiaoyan 6Triticum aestivum-leavesChina:shannxi
SRR8374806cDNAT1seedling stageleavesXiaoyan 6Triticum aestivum-leavesChina:shannxi

Run LibrarySelection Library Name Tissue Cultivar Sample Name geo_loc_name Replicate
SRR7550252RANDOMwheat_mock_14dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550253RANDOMwheat_mock_12dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550254RANDOMwheat_mock_14dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550255RANDOMwheat_mock_14dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550256RANDOMwheat_mock_7dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550257RANDOMwheat_mock_7dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550258RANDOMwheat_mock_12dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550259RANDOMwheat_mock_12dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550270RANDOM3D7_14dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550271RANDOM3D7_14dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550272RANDOM3D7_12dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550273RANDOM3D7_12dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550274RANDOM3D7_12dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550275RANDOM3D7_7dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550276RANDOM3D7_7dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550277RANDOM3D7_7dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550278RANDOMwheat_mock_7dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550279RANDOM3D7_14dpi_rep3LeavesDrifterwheatSwitzerlandrep3

Run LibrarySelection Biome Sample Name geo_loc_name Environment
ERR2235370RANDOMrachisSAMEA104455176Canadagreenhouse
ERR2235369RANDOMrachisSAMEA104455175Canadagreenhouse
ERR2235368RANDOMrachisSAMEA104455174Canadagreenhouse
ERR2235367RANDOMrachisSAMEA104455173Canadagreenhouse
ERR2235366RANDOMrachisSAMEA104455172Canadagreenhouse
ERR2235365RANDOMrachisSAMEA104455171Canadagreenhouse
ERR2235364RANDOMspikeletSAMEA104455170Canadagreenhouse
ERR2235363RANDOMspikeletSAMEA104455169Canadagreenhouse
ERR2235362RANDOMspikeletSAMEA104455168Canadagreenhouse
ERR2235361RANDOMspikeletSAMEA104455167Canadagreenhouse
ERR2235360RANDOMspikeletSAMEA104455166Canadagreenhouse
ERR2235359RANDOMspikeletSAMEA104455165Canadagreenhouse
ERR2235358RANDOMrachisSAMEA104455164Canadagreenhouse
ERR2235357RANDOMrachisSAMEA104455163Canadagreenhouse
ERR2235356RANDOMrachisSAMEA104455162Canadagreenhouse
ERR2235355RANDOMrachisSAMEA104455161Canadagreenhouse
ERR2235354RANDOMrachisSAMEA104455160Canadagreenhouse
ERR2235353RANDOMrachisSAMEA104455159Canadagreenhouse
ERR2235352RANDOMspikeletSAMEA104455158Canadagreenhouse
ERR2235351RANDOMspikeletSAMEA104455157Canadagreenhouse
ERR2235350RANDOMspikeletSAMEA104455156Canadagreenhouse
ERR2235349RANDOMspikeletSAMEA104455155Canadagreenhouse
ERR2235348RANDOMspikeletSAMEA104455154Canadagreenhouse
ERR2235347RANDOMspikeletSAMEA104455153Canadagreenhouse

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Treatment
SRR6328644RT-PCRSR4Athree leaves stagerootSR4sRNAseq_9China:shandongpH8.9
SRR6328645RT-PCRJNAthree leaves stagerootJN177sRNAseq_3China:shandongpH8.9
SRR6328646RT-PCRSR3Cthree leaves stagerootSR3sRNAseq_4China:shandongcontrol
SRR6328647RT-PCRJNCthree leaves stagerootJN177sRNAseq_1China:shandongcontrol
SRR6328648RT-PCRJNSthree leaves stagerootJN177sRNAseq_2China:shandong200mM NaCl
SRR6328649RT-PCRSR4Cthree leaves stagerootSR4sRNAseq_7China:shandongcontrol
SRR6328650RT-PCRSR4Sthree leaves stagerootSR4sRNAseq_8China:shandong200mM NaCl
SRR6328651RT-PCRSR3Sthree leaves stagerootSR3sRNAseq_5China:shandong200mM NaCl
SRR6328652RT-PCRSR3Athree leaves stagerootSR3sRNAseq_6China:shandongpH8.9

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name Replicate
SRR5944333size fractionationWT-sRNA-1HAI-rep11-12 HAIgerminating seedJimai5265WT_JMrep1
SRR5944334size fractionationWT-sRNA-1HAI-rep21-12 HAIgerminating seedJimai5265WT_JMrep2
SRR5944335size fractionationWT-sRNA-6HAI-rep11-12 HAIgerminating seedJimai5265WT_JMrep1
SRR5944336size fractionationWT-sRNA-6HAI-rep21-12 HAIgerminating seedJimai5265WT_JMrep2
SRR5944337size fractionationWT-sRNA-12HAI-rep11-12 HAIgerminating seedJimai5265WT_JMrep1
SRR5944338size fractionationWT-sRNA-12HAI-rep21-12 HAIgerminating seedJimai5265WT_JMrep2

Run LibrarySelection Stage Tissue Sample Name geo_loc_name Treatment
SRR5816454RANDOM24hrootwr_173China:BeijingCa2+-channel blocker

Run LibrarySelection Stage Tissue Cultivar Sample Name geo_loc_name Replicate
SRR5461177cDNATSSpikeChinese SpringTS-2Chinabiological replicate 2
SRR5461176cDNATSSpikeChinese SpringTS-1Chinabiological replicate 1
SRR5460972cDNAAMSpikeChinese SpringAM-2Chinabiological replicate 2
SRR5460967cDNAAMSpikeChinese SpringAM-1Chinabiological replicate 1
SRR5460949cDNAFMSpikeChinese SpringFM-2Chinabiological replicate 2
SRR5460941cDNAFMSpikeChinese SpringFM-1Chinabiological replicate 1
SRR5460939cDNADRSpikeChinese SpringDR-2Chinabiological replicate 2
SRR5460930cDNADRSpikeChinese SpringDR-1Chinabiological replicate 1

Run Library Name Stage Tissue Cultivar Sample Name geo_loc_name
SRR5381543CSAantherantherChinese SpringTriticum aestivum L. Chinese Spring (CS)China
SRR5381542CS-3CAantherantherChinese SpringChinese Spring-gametocidal 3C chromosome monosomic addition line (CS-3C)China

Run LibrarySelection Library Name Age Tissue Cultivar Sample Name geo_loc_name Treatment Replicate
SRR5270350size fractionation12dpi_C_R117 daysFirst expanded leafProINTA Federal12dpi_C_R1Argentina: CordobaControl 12 dpi Replicate 1Replicate 1
SRR5270349size fractionation12dpi_C_R217 daysFirst expanded leafProINTA Federal12dpi_C_R2Argentina: CordobaControl 12 dpi Replicate 2Replicate 2
SRR5270348size fractionation12dpi_T_R117 daysFirst expanded leafProINTA Federal12dpi_T_R1Argentina: CordobaInfected 12 dpi Replicate 1Replicate 1
SRR5270347size fractionation12dpi_T_R217 daysFirst expanded leafProINTA Federal12dpi_T_R2Argentina: CordobaInfected 12 dpi Replicate 2Replicate 2
SRR5270346size fractionation21dpi_C_R126 daysFirst expanded leafProINTA Federal21dpi_C_R1Argentina: CordobaControl 21 dpi Replicate 1Replicate 1
SRR5270345size fractionation21dpi_C_R226 daysFirst expanded leafProINTA Federal21dpi_C_R2Argentina: CordobaControl 21 dpi Replicate 2Replicate 2
SRR5270344size fractionation21dpi_T_R126 daysFirst expanded leafProINTA Federal21dpi_T_R1Argentina: CordobaInfected 21 dpi Replicate 1Replicate 1
SRR5270343size fractionation21dpi_T_R226 daysFirst expanded leafProINTA Federal21dpi_T_R2Argentina: CordobaInfected 21 dpi Replicate 2Replicate 2

Run LibrarySelection Library Name Age Tissue Cultivar Sample Name geo_loc_name Replicate
SRR5096892size fractionationsRNA_DDAA130 daysLeaf 30DTQ27 X TMU38sRNA_DDAA1China:Beijingrep1
SRR5096890size fractionationsRNA_DDAA230 daysLeaf 31DTQ27 X TMU38sRNA_DDAA2China:Beijingrep2
SRR5096872size fractionationsRNA_AADD130 daysLeaf 32DTMU38 X TQ27sRNA_AADD1China:Beijingrep1
SRR5096869size fractionationsRNA_AADD230 daysLeaf 33DTMU38 X TQ27sRNA_AADD2China:Beijingrep2
SRR5096896size fractionationsRNA_AA230 daysLeaf 30DTMU38sRNA_AA2China:Beijing-
SRR5096879size fractionationsRNA_AA130 daysLeaf 30DTMU38sRNA_AA1China:Beijing-
SRR5096877size fractionationsRNA_DD130 daysLeaf 30DTQ27sRNA_DD1China:Beijing-
SRR5096871size fractionationsRNA_DD230 daysLeaf 30DTQ27sRNA_DD2China:Beijing-

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Replicate
SRR4417233size fractionationL21AMature seedSeedL21L21Chinarep1
SRR4417232size fractionationJ22CMature seedSeedJ22J22Chinarep3
SRR4417231size fractionationJ22BMature seedSeedJ22J22Chinarep2
SRR4417230size fractionationJ22AMature seedSeedJ22J22Chinarep1
SRR4417229size fractionationJ19CMature seedSeedJ19J19Chinarep3
SRR4417228size fractionationJ19BMature seedSeedJ19J19Chinarep2
SRR4417227size fractionationJ19AMature seedSeedJ19J19Chinarep1
SRR4417226size fractionationT349CMature seedSeedT349T349Chinarep3
SRR4417225size fractionationL21CMature seedSeedL21L21Chinarep3
SRR4417224size fractionationL21BMature seedSeedL21L21Chinarep2
SRR4417223size fractionationT349BMature seedSeedT349T349Chinarep2
SRR4417222size fractionationT349AMature seedSeedT349T349Chinarep1

Run LibrarySelection Library Name genotype Stage Tissue Phenotype Sample Name geo_loc_name Replicate
SRR4114480RANDOM7294_sRNASeq7294bootingLeaf sheathglaucous7294_sRNASeqCanada: Saskatchewan-
SRR4114479RANDOM7293_sRNASeq7293bootingLeaf sheathglaucous7293_sRNASeqCanada: Saskatchewan-
SRR4114478RANDOM7290_sRNASeq7290bootingLeaf sheathglaucous7290_sRNASeqCanada: Saskatchewan-
SRR4114477RANDOM7289_sRNASeq7289bootingLeaf sheathglaucous7289_sRNASeqCanada: Saskatchewan-
SRR4114476RANDOM7287_sRNASeq7287bootingLeaf sheathnon-glaucous7287_sRNASeqCanada: Saskatchewan-
SRR4114475RANDOM7285_sRNASeq7285bootingLeaf sheathnon-glaucous7285_sRNASeqCanada: Saskatchewan-
SRR4114473RANDOM7282_sRNASeq7282bootingLeaf sheathnon-glaucous7282_sRNASeqCanada: Saskatchewan-
SRR4114472RANDOM7281_sRNASeq7281bootingLeaf sheathnon-glaucous7281_sRNASeqCanada: Saskatchewan-
SRR4114471RANDOM7279_sRNASeq7279bootingLeaf sheathnon-glaucous7279_sRNASeqCanada: Saskatchewan-
SRR4114460RANDOMD051N-2_sRNASeqD051NbootingLeaf sheathnon-glaucousD051N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114459RANDOMD051N-1_sRNASeqD051NbootingLeaf sheathnon-glaucousD051N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114458RANDOMD051-2_sRNASeqD051bootingLeaf sheathglaucousD051-2_sRNASeqCanada: Saskatchewanrep2
SRR4114457RANDOMD051-1_sRNASeqD051bootingLeaf sheathglaucousD051-1_sRNASeqCanada: Saskatchewanrep1
SRR4114456RANDOMAG2N-4_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-4_sRNASeqCanada: Saskatchewanrep4
SRR4114455RANDOMAG2N-3_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-3_sRNASeqCanada: Saskatchewanrep3
SRR4114454RANDOMAG2N-2_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114453RANDOMAG2N-1_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114451RANDOMAG2F1-2_sRNASeqAG2F1bootingLeaf sheathnon-glaucousAG2F1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114450RANDOMAG2F1-1_sRNASeqAG2F1bootingLeaf sheathnon-glaucousAG2F1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114449RANDOMAG2-4_sRNASeqAG2bootingLeaf sheathglaucousAG2-4_sRNASeqCanada: Saskatchewanrep4
SRR4114448RANDOMAG2-3_sRNASeqAG2bootingLeaf sheathglaucousAG2-3_sRNASeqCanada: Saskatchewanrep3
SRR4114447RANDOMAG2-2_sRNASeqAG2bootingLeaf sheathglaucousAG2-2_sRNASeqCanada: Saskatchewanrep2
SRR4114446RANDOMAG2-2_F1C_sRNASeqAG2_F1CbootingLeaf sheathglaucousAG2-2_F1C_sRNASeqCanada: Saskatchewanrep2
SRR4114445RANDOMAG2-1_sRNASeqAG2bootingLeaf sheathglaucousAG2-1_sRNASeqCanada: Saskatchewanrep1
SRR4114444RANDOMAG2-1_F1C_sRNASeqAG2_F1CbootingLeaf sheathglaucousAG2-1_F1C_sRNASeqCanada: Saskatchewanrep1
SRR4114443RANDOMAG1N-2_sRNASeqAG1NbootingLeaf sheathnon-glaucousAG1N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114442RANDOMAG1N-1_sRNASeqAG1NbootingLeaf sheathnon-glaucousAG1N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114440RANDOMAG1F1-2_sRNASeqAG1F1bootingLeaf sheathnon-glaucousAG1F1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114439RANDOMAG1F1-1_sRNASeqAG1F1bootingLeaf sheathnon-glaucousAG1F1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114438RANDOMAG1-2_sRNASeqAG1bootingLeaf sheathglaucousAG1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114437RANDOMAG1-1_sRNASeqAG1bootingLeaf sheathglaucousAG1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114436RANDOMAE3N-2_sRNASeqAE3NbootingLeaf sheathnon-glaucousAE3N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114435RANDOMAE3N-1_sRNASeqAE3NbootingLeaf sheathnon-glaucousAE3N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114434RANDOMAE3F1-2_sRNASeqAE3F1bootingLeaf sheathnon-glaucousAE3F1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114433RANDOMAE3F1-1_sRNASeqAE3F1bootingLeaf sheathnon-glaucousAE3F1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114432RANDOMAE3-2_sRNASeqAE3bootingLeaf sheathglaucousAE3-2_sRNASeqCanada: Saskatchewanrep2
SRR4114431RANDOMAE3-1_sRNASeqAE3bootingLeaf sheathglaucousAE3-1_sRNASeqCanada: Saskatchewanrep1

Run LibrarySelection SAMPLING_TIME Cultivar Sample Name Treatment Replicate
SRR3721412size fractionation10 day after treatment (DAT)GlenleaGSM2214310UVRep3
SRR3721411size fractionation10 day after treatment (DAT)GlenleaGSM2214309LightRep3
SRR3721410size fractionation10 day after treatment (DAT)GlenleaGSM2214308HeatRep3
SRR3721409size fractionation10 day after treatment (DAT)GlenleaGSM2214307ControlRep3
SRR3721408size fractionation10 day after treatment (DAT)GlenleaGSM2214306UVRep2
SRR3721407size fractionation10 day after treatment (DAT)GlenleaGSM2214305LightRep2
SRR3721406size fractionation10 day after treatment (DAT)GlenleaGSM2214304HeatRep2
SRR3721405size fractionation10 day after treatment (DAT)GlenleaGSM2214303ControlRep2
SRR3721404size fractionation10 day after treatment (DAT)GlenleaGSM2214302UVRep1
SRR3721403size fractionation10 day after treatment (DAT)GlenleaGSM2214301LightRep1
SRR3721402size fractionation10 day after treatment (DAT)GlenleaGSM2214300HeatRep1
SRR3721401size fractionation10 day after treatment (DAT)GlenleaGSM2214299ControlRep1
SRR3721400size fractionation7 day after treatment (DAT)GlenleaGSM2214298UVRep3
SRR3721399size fractionation7 day after treatment (DAT)GlenleaGSM2214297LightRep3
SRR3721398size fractionation7 day after treatment (DAT)GlenleaGSM2214296HeatRep3
SRR3721397size fractionation7 day after treatment (DAT)GlenleaGSM2214295ControlRep3
SRR3721396size fractionation7 day after treatment (DAT)GlenleaGSM2214294UVRep2
SRR3721395size fractionation7 day after treatment (DAT)GlenleaGSM2214293LightRep2
SRR3721394size fractionation7 day after treatment (DAT)GlenleaGSM2214292HeatRep2
SRR3721393size fractionation7 day after treatment (DAT)GlenleaGSM2214291ControlRep2
SRR3721392size fractionation7 day after treatment (DAT)GlenleaGSM2214290UVRep1
SRR3721391size fractionation7 day after treatment (DAT)GlenleaGSM2214289LightRep1
SRR3721390size fractionation7 day after treatment (DAT)GlenleaGSM2214288HeatRep1
SRR3721389size fractionation7 day after treatment (DAT)GlenleaGSM2214287ControlRep1
SRR3721388size fractionation3 day after treatment (DAT)GlenleaGSM2214286UVRep3
SRR3721387size fractionation3 day after treatment (DAT)GlenleaGSM2214285LightRep3
SRR3721386size fractionation3 day after treatment (DAT)GlenleaGSM2214284HeatRep3
SRR3721385size fractionation3 day after treatment (DAT)GlenleaGSM2214283ControlRep3
SRR3721384size fractionation3 day after treatment (DAT)GlenleaGSM2214282UVRep2
SRR3721383size fractionation3 day after treatment (DAT)GlenleaGSM2214281LightRep2
SRR3721382size fractionation3 day after treatment (DAT)GlenleaGSM2214280HeatRep2
SRR3721381size fractionation3 day after treatment (DAT)GlenleaGSM2214279ControlRep2
SRR3721380size fractionation3 day after treatment (DAT)GlenleaGSM2214278UVRep1
SRR3721379size fractionation3 day after treatment (DAT)GlenleaGSM2214277LightRep1
SRR3721378size fractionation3 day after treatment (DAT)GlenleaGSM2214276HeatRep1
SRR3721377size fractionation3 day after treatment (DAT)GlenleaGSM2214275ControlRep1
SRR3721376size fractionation2 day after treatment (DAT)GlenleaGSM2214274UVRep3
SRR3721375size fractionation2 day after treatment (DAT)GlenleaGSM2214273LightRep3
SRR3721374size fractionation2 day after treatment (DAT)GlenleaGSM2214272HeatRep3
SRR3721373size fractionation2 day after treatment (DAT)GlenleaGSM2214271ControlRep3
SRR3721372size fractionation2 day after treatment (DAT)GlenleaGSM2214270UVRep2
SRR3721371size fractionation2 day after treatment (DAT)GlenleaGSM2214269LightRep2
SRR3721370size fractionation2 day after treatment (DAT)GlenleaGSM2214268HeatRep2
SRR3721369size fractionation2 day after treatment (DAT)GlenleaGSM2214267ControlRep2
SRR3721368size fractionation2 day after treatment (DAT)GlenleaGSM2214266UVRep1
SRR3721367size fractionation2 day after treatment (DAT)GlenleaGSM2214265LightRep1
SRR3721366size fractionation2 day after treatment (DAT)GlenleaGSM2214264HeatRep1
SRR3721365size fractionation2 day after treatment (DAT)GlenleaGSM2214263ControlRep1
SRR3721364size fractionation1 day after treatment (DAT)GlenleaGSM2214262UVRep3
SRR3721363size fractionation1 day after treatment (DAT)GlenleaGSM2214261LightRep3
SRR3721362size fractionation1 day after treatment (DAT)GlenleaGSM2214260HeatRep3
SRR3721361size fractionation1 day after treatment (DAT)GlenleaGSM2214259ControlRep3
SRR3721360size fractionation1 day after treatment (DAT)GlenleaGSM2214258UVRep2
SRR3721359size fractionation1 day after treatment (DAT)GlenleaGSM2214257LightRep2
SRR3721358size fractionation1 day after treatment (DAT)GlenleaGSM2214256HeatRep2
SRR3721357size fractionation1 day after treatment (DAT)GlenleaGSM2214255ControlRep2
SRR3721356size fractionation1 day after treatment (DAT)GlenleaGSM2214254UVRep1
SRR3721355size fractionation1 day after treatment (DAT)GlenleaGSM2214253LightRep1
SRR3721354size fractionation1 day after treatment (DAT)GlenleaGSM2214252HeatRep1
SRR3721353size fractionation1 day after treatment (DAT)GlenleaGSM2214251ControlRep1
SRR3721352size fractionation0 day after treatment (DAT)GlenleaGSM2214250UVRep3
SRR3721351size fractionation0 day after treatment (DAT)GlenleaGSM2214249LightRep3
SRR3721350size fractionation0 day after treatment (DAT)GlenleaGSM2214248HeatRep3
SRR3721349size fractionation0 day after treatment (DAT)GlenleaGSM2214247ControlRep3
SRR3721348size fractionation0 day after treatment (DAT)GlenleaGSM2214246UVRep2
SRR3721347size fractionation0 day after treatment (DAT)GlenleaGSM2214245LightRep2
SRR3721346size fractionation0 day after treatment (DAT)GlenleaGSM2214244HeatRep2
SRR3721345size fractionation0 day after treatment (DAT)GlenleaGSM2214243ControlRep2
SRR3721344size fractionation0 day after treatment (DAT)GlenleaGSM2214242UVRep1
SRR3721343size fractionation0 day after treatment (DAT)GlenleaGSM2214241LightRep1
SRR3721342size fractionation0 day after treatment (DAT)GlenleaGSM2214240HeatRep1
SRR3721341size fractionation0 day after treatment (DAT)GlenleaGSM2214239ControlRep1

Run LibrarySelection Stage Tissue Cultivar Sample Name geo_loc_name
SRR3144539size fractionationafter 15 days culture derived from immature embryocallusZhoumai18IME15 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3144538size fractionationafter 6 days culture derived from immature embryocallusZhoumai18IME6 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3144523size fractionationafter 3 days culture derived from immature embryocallusZhoumai18IME3 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3144511size fractionationafter 6 days culture dereved from mature embryocallusZhoumai 18ME6 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3140978size fractionationafter15 days culture derived from mature embryowheat callusZhoumai 18ME15 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3140846size fractionationafter 3 days culture dereved from mature embryocallusZhoumai 18ME3 sRNA libraryChina: Henan Agricultural University, Zhengzhou

Experiment Accession Run LibrarySelection Age Tissue Cultivar Sample Name geo_loc_name
SRX1548275SRR3151453 SRR3151454size fractionation30 daysgrainSpeltaI_Spelta_30DHungary
SRX1548274SRR3151451 SRR3151452size fractionation20 daysgrainSpeltaI_Spelta_20DHungary
SRX1548273SRR3151449 SRR3151450size fractionation10 daysgrainSpeltaI_Spelta_10DHungary
SRX1548272SRR3151447 SRR3151448size fractionation30 daysgrainBankuti lines B52I_B52_30DHungary
SRX1548271SRR3151445 SRR3151446size fractionation20 daysgrainBankuti lines B52I_B52_20DHungary
SRX1548269SRR3151443 SRR3151444size fractionation10 daysgrainBankuti lines B52I_B52_10DHungary
SRX1548268SRR3151441 SRR3151442size fractionation30 daysgrainBankuti lines B35I_B35_30DHungary
SRX1548266SRR3151439 SRR3151440size fractionation20 daysgrainBankuti lines B35I_B35_20DHungary
SRX1548265SRR3151437 SRR3151438size fractionation10 daysgrainBankuti lines B35I_B35_10DHungary
SRX1548199SRR3151435 SRR3151436RANDOM30 daysgrainSpeltaT_Spelta_30DHungary
SRX1548198SRR3151433 SRR3151434RANDOM20 daysgrainSpeltaT_Spelta_20DHungary
SRX1548192SRR3151431 SRR3151432RANDOM10 daysgrainSpeltaT_Spelta_10DHungary
SRX1548177SRR3151429 SRR3151430RANDOM30 daysgrainBankuti lines B52T_B52_30DHungary
SRX1548164SRR3151427 SRR3151428RANDOM20 daysgrainBankuti lines B52T_B52_20DHungary
SRX1548163SRR3151425 SRR3151426RANDOM10 daysgrainBankuti lines B52T_B52_10DHungary
SRX1548152SRR3144555 SRR3151424RANDOM30 daysgrainBankuti lines B35T_B35_30DHungary
SRX1548148SRR3144553 SRR3144554RANDOM20 daysgrainBankuti lines B35T_B35_20DHungary
SRX1546448SRR3144551 SRR3144552RANDOM10 daysgrainBankuti lines B35T_B35_10DHungary

Run LibrarySelection Library Name Stage Tissue Cultivar Sample Name geo_loc_name Treatment
SRR3136704RANDOMSKS-Sseed germinationShootHD-2967Shoot tissueIndiaNitrate stress
SRR2146991RANDOMSKS-NSeedling stageRootHD-2967Root tisueIndiaNitrate stress
SRR2146992RANDOMSKS-SSeedling stageRootHD-2967Root tisueIndiaNitrate stress

Run LibrarySelection Age/Stage Tissue Cultivar Sample Name
SRR1796322size fractionation28 days post anthesisgrainChinese SpringGSM1606477
SRR1796321size fractionation21 days post anthesisgrainChinese SpringGSM1606476
SRR1796320size fractionation14 days post anthesisgrainChinese SpringGSM1606475
SRR1796319size fractionation7 days post anthesisgrainChinese SpringGSM1606474

Run LibrarySelection Stage Tissue Cultivar Sample Name
SRR1728653size fractionationtwo-leaf stageleafZhengyin1T2 sRNA library
SRR1728566size fractionationtwo-leaf stageleafZhengyin1C2 sRNA library
SRR1727004size fractionationtwo-leaf stageleafHanxuan10C1
SRR1728309size fractionationtwo-leaf stageleafHanxuan 10T1 sRNA library

Run LibrarySelection Library Name Age Tissue Cultivar Sample Name geo_loc_name
SRR1646809size fractionationR-PImi_data.fastq10 daysleafHD2329+Lr24Small RNA Wheat HD2329+Lr24 infected with Puccinia triticina (R-PImi)India
SRR1646808size fractionationR-Mmi_data.fastq10 daysleafHD2329+Lr24Small RNA Wheat HD2329+Lr24 mock inoculated (R-Mmi)India
SRR1646807size fractionationS-PImi_data.fastq10 daysleafHD2329Small RNA Wheat HD2329 infected with Puccinia triticina (S-PImi)India
SRR1646806size fractionationSmall RNA Wheat HD2329 mock inoculated (S-Mmi)10 daysleafHD2329Small RNA Wheat HD2329 mock inoculated (S-Mmi)India

Experiment Accession Run Cultivar Sample Name
SRX500281SRR1203861 SRR1203865 SRR1203916 SRR1203917wheatIdentification of Novel miRNAs and miRNA Expression Profiling in Wheat Hybrid Necrosis

Run LibrarySelection Tissue Cultivar Sample Name
SRR1196023size fractionationflag leavesChinese SpringFL
SRR1196045size fractionationgrain of 15 days after pollinationChinese SpringGRA15
SRR1196029size fractionationgrain of 8 days after pollinationChinese SpringGRA8
SRR1196022size fractionation10-15 mm young spikesChinese SpringYS15
SRR1195944size fractionation0-5 mm young spikesChinese SpringYS5
SRR1195282size fractionationstems in the jointing stageChinese SpringSJ
SRR1195281size fractionationseedling rootChinese SpringSR
SRR1195280size fractionationseedling leafChinese Springseedling leaf
SRR1195279size fractionationseedling shootChinese SpringSH
SRR1195025size fractionationembryo of germinating seed embryosChinese SpringGSE
SRR1195024size fractionationgrainsChinese Springdry grains

Run LibrarySelection Sample Name
SRR800158RANDOM PCRHD2985_treated
SRR800157RANDOMHD2985_control

Run LibrarySelection Genotype Tissue sub_species Sample Name Treatment
SRR14128339size fractionationDBA AuroraDeveloping grain tissuedurumGSM5222605Heat stress group
SRR14128338size fractionationDBA AuroraDeveloping grain tissuedurumGSM5222604Control group
SRR14128337size fractionationDBA AuroraDeveloping grain tissuedurumGSM5222603Heat stress group
SRR14128336size fractionationDBA AuroraDeveloping grain tissuedurumGSM5222602Control group
SRR14128335size fractionationDBA AuroraFlag leaf tissuedurumGSM5222601Heat stress group
SRR14128334size fractionationDBA AuroraFlag leaf tissuedurumGSM5222600Control group
SRR14128333size fractionationDBA AuroraFlag leaf tissuedurumGSM5222599Heat stress group
SRR14128332size fractionationDBA AuroraFlag leaf tissuedurumGSM5222598Control group

Run LibrarySelection Genotype sub_species Sample Name Treatment
SRR13833550size fractionationL6 (UAD1301020-8)durumGSM5128902Nitrogen starvation group
SRR13833549size fractionationL6 (UAD1301020-8)durumGSM5128901Control group
SRR13833548size fractionationL6 (UAD1301020-8)durumGSM5128900Nitrogen starvation group
SRR13833547size fractionationL6 (UAD1301020-8)durumGSM5128899Control group
SRR13833546size fractionationDBA AuroradurumGSM5128898Nitrogen starvation group
SRR13833545size fractionationDBA AuroradurumGSM5128897Control group
SRR13833544size fractionationDBA AuroradurumGSM5128896Nitrogen starvation group
SRR13833543size fractionationDBA AuroradurumGSM5128895Control group

Run LibrarySelection Genotype sub_species Sample Name Treatment
SRR13114338size fractionationDBA ArtemisdurumGSM4929666Water-deficit stress group
SRR13114337size fractionationDBA ArtemisdurumGSM4929665Control group
SRR13114336size fractionationDBA ArtemisdurumGSM4929664Water-deficit stress group
SRR13114335size fractionationDBA ArtemisdurumGSM4929663Control group
SRR13114334size fractionationDBA ArtemisdurumGSM4929662Water-deficit stress group
SRR13114333size fractionationDBA ArtemisdurumGSM4929661Control group
SRR13114332size fractionationDBA ArtemisdurumGSM4929660Water-deficit stress group
SRR13114331size fractionationDBA ArtemisdurumGSM4929659Control group

Run LibrarySelection Genotype sub_species Sample Name Treatment Replicate
SRR12164786size fractionation-durumGSM4658665-Rep1
SRR12164785size fractionation-durumGSM4658664-Rep2
SRR12164784size fractionation-durumGSM4658663-Rep1
SRR12164783size fractionationL6 (Stres-sensitive)durumGSM4658662Control group (CG)Rep3
SRR12164782size fractionationL6 (Stres-sensitive)durumGSM4658661Control group (CG)Rep2
SRR12164781size fractionationL6 (Stres-sensitive)durumGSM4658660Control group (CG)Rep1
SRR12164780size fractionationL6 (Stres-sensitive)durumGSM4658659Water-deficit plus heat stress (WH)Rep3
SRR12164779size fractionationL6 (Stres-sensitive)durumGSM4658658Water-deficit plus heat stress (WH)Rep2
SRR12164778size fractionationL6 (Stres-sensitive)durumGSM4658657Water-deficit plus heat stress (WH)Rep1
SRR12164777size fractionationL6 (Stres-sensitive)durumGSM4658656Heat stress (HS)Rep3
SRR12164776size fractionationL6 (Stres-sensitive)durumGSM4658655Heat stress (HS)Rep2
SRR12164775size fractionationL6 (Stres-sensitive)durumGSM4658654Heat stress (HS)Rep1
SRR12164774size fractionationL6 (Stres-sensitive)durumGSM4658653Water-deficit stress (WS)Rep3
SRR12164773size fractionationL6 (Stres-sensitive)durumGSM4658652Water-deficit stress (WS)Rep2
SRR12164772size fractionationL6 (Stres-sensitive)durumGSM4658651Water-deficit stress (WS)Rep1
SRR12164771size fractionationL6 (Stres-sensitive)durumGSM4658650Control group (CG)Rep3
SRR12164770size fractionationL6 (Stres-sensitive)durumGSM4658649Control group (CG)Rep2
SRR12164769size fractionationL6 (Stres-sensitive)durumGSM4658648Control group (CG)Rep1
SRR12164768size fractionationL6 (Stres-sensitive)durumGSM4658647Water-deficit plus heat stress (WH)Rep3
SRR12164767size fractionationL6 (Stres-sensitive)durumGSM4658646Water-deficit plus heat stress (WH)Rep2
SRR12164766size fractionationL6 (Stres-sensitive)durumGSM4658645Water-deficit plus heat stress (WH)Rep1
SRR12164765size fractionationL6 (Stres-sensitive)durumGSM4658644Heat stress (HS)Rep3
SRR12164764size fractionationL6 (Stres-sensitive)durumGSM4658643Heat stress (HS)Rep2
SRR12164763size fractionationL6 (Stres-sensitive)durumGSM4658642Heat stress (HS)Rep1
SRR12164762size fractionationL6 (Stres-sensitive)durumGSM4658641Water-deficit stress (WS)Rep3
SRR12164761size fractionationL6 (Stres-sensitive)durumGSM4658640Water-deficit stress (WS)Rep2
SRR12164760size fractionationL6 (Stres-sensitive)durumGSM4658639Water-deficit stress (WS)Rep1
SRR12164759size fractionationL6 (Stres-sensitive)durumGSM4658638Control group (CG)Rep3
SRR12164758size fractionationL6 (Stres-sensitive)durumGSM4658637Control group (CG)Rep2
SRR12164757size fractionationL6 (Stres-sensitive)durumGSM4658636Control group (CG)Rep1
SRR12164756size fractionationL6 (Stres-sensitive)durumGSM4658635Water-deficit plus heat stress (WH)Rep3
SRR12164755size fractionationL6 (Stres-sensitive)durumGSM4658634Water-deficit plus heat stress (WH)Rep2
SRR12164754size fractionationL6 (Stres-sensitive)durumGSM4658633Water-deficit plus heat stress (WH)Rep1
SRR12164753size fractionationL6 (Stres-sensitive)durumGSM4658632Heat stress (HS)Rep3
SRR12164752size fractionationL6 (Stres-sensitive)durumGSM4658631Heat stress (HS)Rep2
SRR12164751size fractionationL6 (Stres-sensitive)durumGSM4658630Heat stress (HS)Rep1
SRR12164750size fractionationL6 (Stres-sensitive)durumGSM4658629Water-deficit stress (WS)Rep3
SRR12164749size fractionationL6 (Stres-sensitive)durumGSM4658628Water-deficit stress (WS)Rep2
SRR12164748size fractionationL6 (Stres-sensitive)durumGSM4658627Water-deficit stress (WS)Rep1
SRR12164747size fractionationL6 (Stres-sensitive)durumGSM4658626Control group (CG)Rep3
SRR12164746size fractionationL6 (Stres-sensitive)durumGSM4658625Control group (CG)Rep2
SRR12164745size fractionationL6 (Stres-sensitive)durumGSM4658624Control group (CG)Rep1
SRR12164744size fractionationL6 (Stres-sensitive)durumGSM4658623Water-deficit plus heat stress (WH)Rep3
SRR12164743size fractionationL6 (Stres-sensitive)durumGSM4658622Water-deficit plus heat stress (WH)Rep2
SRR12164742size fractionationL6 (Stres-sensitive)durumGSM4658621Water-deficit plus heat stress (WH)Rep1
SRR12164741size fractionationL6 (Stres-sensitive)durumGSM4658620Heat stress (HS)Rep3
SRR12164740size fractionationL6 (Stres-sensitive)durumGSM4658619Heat stress (HS)Rep2
SRR12164739size fractionationL6 (Stres-sensitive)durumGSM4658618Heat stress (HS)Rep1
SRR12164738size fractionationL6 (Stres-sensitive)durumGSM4658617Water-deficit stress (WS)Rep3
SRR12164737size fractionationL6 (Stres-sensitive)durumGSM4658616Water-deficit stress (WS)Rep2
SRR12164736size fractionationL6 (Stres-sensitive)durumGSM4658615Water-deficit stress (WS)Rep1
SRR12164735size fractionationL6 (Stres-sensitive)durumGSM4658614Control group (CG)Rep3
SRR12164734size fractionationL6 (Stres-sensitive)durumGSM4658613Control group (CG)Rep2
SRR12164733size fractionationL6 (Stres-sensitive)durumGSM4658612Control group (CG)Rep1
SRR12164732size fractionationDBA Aurora (Stress-tolerant)durumGSM4658611Water-deficit plus heat stress (WH)Rep3
SRR12164731size fractionationDBA Aurora (Stress-tolerant)durumGSM4658610Water-deficit plus heat stress (WH)Rep2
SRR12164730size fractionationDBA Aurora (Stress-tolerant)durumGSM4658609Water-deficit plus heat stress (WH)Rep1
SRR12164729size fractionationDBA Aurora (Stress-tolerant)durumGSM4658608Heat stress (HS)Rep3
SRR12164728size fractionationDBA Aurora (Stress-tolerant)durumGSM4658607Heat stress (HS)Rep2
SRR12164727size fractionationDBA Aurora (Stress-tolerant)durumGSM4658606Heat stress (HS)Rep1
SRR12164726size fractionationDBA Aurora (Stress-tolerant)durumGSM4658605Water-deficit stress (WS)Rep3
SRR12164725size fractionationDBA Aurora (Stress-tolerant)durumGSM4658604Water-deficit stress (WS)Rep2
SRR12164724size fractionationDBA Aurora (Stress-tolerant)durumGSM4658603Water-deficit stress (WS)Rep1
SRR12164723size fractionationDBA Aurora (Stress-tolerant)durumGSM4658602Control group (CG)Rep3
SRR12164722size fractionationDBA Aurora (Stress-tolerant)durumGSM4658601Control group (CG)Rep2
SRR12164721size fractionationDBA Aurora (Stress-tolerant)durumGSM4658600Control group (CG)Rep1
SRR12164720size fractionationDBA Aurora (Stress-tolerant)durumGSM4658599Water-deficit plus heat stress (WH)Rep3
SRR12164719size fractionationDBA Aurora (Stress-tolerant)durumGSM4658598Water-deficit plus heat stress (WH)Rep2
SRR12164718size fractionationDBA Aurora (Stress-tolerant)durumGSM4658597Water-deficit plus heat stress (WH)Rep1
SRR12164717size fractionationDBA Aurora (Stress-tolerant)durumGSM4658596Heat stress (HS)Rep3
SRR12164716size fractionationDBA Aurora (Stress-tolerant)durumGSM4658595Heat stress (HS)Rep2
SRR12164715size fractionationDBA Aurora (Stress-tolerant)durumGSM4658594Heat stress (HS)Rep1
SRR12164714size fractionationDBA Aurora (Stress-tolerant)durumGSM4658593Water-deficit stress (WS)Rep3
SRR12164713size fractionationDBA Aurora (Stress-tolerant)durumGSM4658592Water-deficit stress (WS)Rep2
SRR12164712size fractionationDBA Aurora (Stress-tolerant)durumGSM4658591Water-deficit stress (WS)Rep1
SRR12164711size fractionationDBA Aurora (Stress-tolerant)durumGSM4658590Control group (CG)Rep3
SRR12164710size fractionationDBA Aurora (Stress-tolerant)durumGSM4658589Control group (CG)Rep2
SRR12164709size fractionationDBA Aurora (Stress-tolerant)durumGSM4658588Control group (CG)Rep1
SRR12164708size fractionationDBA Aurora (Stress-tolerant)durumGSM4658587Water-deficit plus heat stress (WH)Rep3
SRR12164707size fractionationDBA Aurora (Stress-tolerant)durumGSM4658586Water-deficit plus heat stress (WH)Rep2
SRR12164706size fractionationDBA Aurora (Stress-tolerant)durumGSM4658585Water-deficit plus heat stress (WH)Rep1
SRR12164705size fractionationDBA Aurora (Stress-tolerant)durumGSM4658584Heat stress (HS)Rep3
SRR12164704size fractionationDBA Aurora (Stress-tolerant)durumGSM4658583Heat stress (HS)Rep2
SRR12164703size fractionationDBA Aurora (Stress-tolerant)durumGSM4658582Heat stress (HS)Rep1
SRR12164702size fractionationDBA Aurora (Stress-tolerant)durumGSM4658581Water-deficit stress (WS)Rep3
SRR12164701size fractionationDBA Aurora (Stress-tolerant)durumGSM4658580Water-deficit stress (WS)Rep2
SRR12164700size fractionationDBA Aurora (Stress-tolerant)durumGSM4658579Water-deficit stress (WS)Rep1
SRR12164699size fractionationDBA Aurora (Stress-tolerant)durumGSM4658578Control group (CG)Rep3
SRR12164698size fractionationDBA Aurora (Stress-tolerant)durumGSM4658577Control group (CG)Rep2
SRR12164697size fractionationDBA Aurora (Stress-tolerant)durumGSM4658576Control group (CG)Rep1
SRR12164696size fractionationDBA Aurora (Stress-tolerant)durumGSM4658575Water-deficit plus heat stress (WH)Rep3
SRR12164695size fractionationDBA Aurora (Stress-tolerant)durumGSM4658574Water-deficit plus heat stress (WH)Rep2
SRR12164694size fractionationDBA Aurora (Stress-tolerant)durumGSM4658573Water-deficit plus heat stress (WH)Rep1
SRR12164693size fractionationDBA Aurora (Stress-tolerant)durumGSM4658572Heat stress (HS)Rep3
SRR12164692size fractionationDBA Aurora (Stress-tolerant)durumGSM4658571Heat stress (HS)Rep2
SRR12164691size fractionationDBA Aurora (Stress-tolerant)durumGSM4658570Heat stress (HS)Rep1
SRR12164690size fractionationDBA Aurora (Stress-tolerant)durumGSM4658569Water-deficit stress (WS)Rep3
SRR12164689size fractionationDBA Aurora (Stress-tolerant)durumGSM4658568Water-deficit stress (WS)Rep2
SRR12164688size fractionationDBA Aurora (Stress-tolerant)durumGSM4658567Water-deficit stress (WS)Rep1
SRR12164687size fractionationDBA Aurora (Stress-tolerant)durumGSM4658566Control group (CG)Rep3
SRR12164686size fractionationDBA Aurora (Stress-tolerant)durumGSM4658565Control group (CG)Rep2
SRR12164685size fractionationDBA Aurora (Stress-tolerant)durumGSM4658564Control group (CG)Rep1
SRR12164684size fractionationDBA Aurora (Stress-tolerant)durumGSM4658563Water-deficit plus heat stress (WH)Rep3
SRR12164683size fractionationDBA Aurora (Stress-tolerant)durumGSM4658562Water-deficit plus heat stress (WH)Rep2
SRR12164682size fractionationDBA Aurora (Stress-tolerant)durumGSM4658561Water-deficit plus heat stress (WH)Rep1
SRR12164681size fractionationDBA Aurora (Stress-tolerant)durumGSM4658560Heat stress (HS)Rep3
SRR12164680size fractionationDBA Aurora (Stress-tolerant)durumGSM4658559Heat stress (HS)Rep2
SRR12164679size fractionationDBA Aurora (Stress-tolerant)durumGSM4658558Heat stress (HS)Rep1
SRR12164678size fractionationDBA Aurora (Stress-tolerant)durumGSM4658557Water-deficit stress (WS)Rep3
SRR12164677size fractionationDBA Aurora (Stress-tolerant)durumGSM4658556Water-deficit stress (WS)Rep2
SRR12164676size fractionationDBA Aurora (Stress-tolerant)durumGSM4658555Water-deficit stress (WS)Rep1
SRR12164675size fractionationDBA Aurora (Stress-tolerant)durumGSM4658554Control group (CG)Rep3
SRR12164790size fractionationL6 (Stres-sensitive)durumGSM4658669Water-deficit plus heat stress (WH)Rep2
SRR12164789size fractionationL6 (Stres-sensitive)durumGSM4658668Water-deficit plus heat stress (WH)Rep1
SRR12164788size fractionationL6 (Stres-sensitive)durumGSM4658667Heat stress (HS)Rep3
SRR12164787size fractionation-durumGSM4658666-Rep2
SRR12164674size fractionationDBA Aurora (Stress-tolerant)durumGSM4658553Control group (CG)Rep2
SRR12164673size fractionationDBA Aurora (Stress-tolerant)durumGSM4658552Control group (CG)Rep1

Run LibrarySelection Genotype sub_species Sample Name Treatment Replicate
SRR12067466size fractionationL6 (Stres-sensitive)durumGSM4630931Water-deficit plus heat stress (WH)Rep3
SRR12067465size fractionationL6 (Stres-sensitive)durumGSM4630930Water-deficit plus heat stress (WH)Rep2
SRR12067464size fractionationL6 (Stres-sensitive)durumGSM4630929Water-deficit plus heat stress (WH)Rep1
SRR12067463size fractionationL6 (Stres-sensitive)durumGSM4630928Heat stress (HS)Rep3
SRR12067462size fractionationL6 (Stres-sensitive)durumGSM4630927Heat stress (HS)Rep2
SRR12067461size fractionationL6 (Stres-sensitive)durumGSM4630926Heat stress (HS)Rep1
SRR12067460size fractionationL6 (Stres-sensitive)durumGSM4630925Water-deficit stress (WS)Rep3
SRR12067459size fractionationL6 (Stres-sensitive)durumGSM4630924Water-deficit stress (WS)Rep2
SRR12067458size fractionationL6 (Stres-sensitive)durumGSM4630923Water-deficit stress (WS)Rep1
SRR12067457size fractionationL6 (Stres-sensitive)durumGSM4630922Control group (CG)Rep3
SRR12067456size fractionationL6 (Stres-sensitive)durumGSM4630921Control group (CG)Rep2
SRR12067455size fractionationL6 (Stres-sensitive)durumGSM4630920Control group (CG)Rep1
SRR12067454size fractionationL6 (Stres-sensitive)durumGSM4630919Water-deficit plus heat stress (WH)Rep3
SRR12067453size fractionationL6 (Stres-sensitive)durumGSM4630918Water-deficit plus heat stress (WH)Rep2
SRR12067452size fractionationL6 (Stres-sensitive)durumGSM4630917Water-deficit plus heat stress (WH)Rep1
SRR12067451size fractionationL6 (Stres-sensitive)durumGSM4630916Heat stress (HS)Rep3
SRR12067450size fractionationL6 (Stres-sensitive)durumGSM4630915Heat stress (HS)Rep2
SRR12067449size fractionationL6 (Stres-sensitive)durumGSM4630914Heat stress (HS)Rep1
SRR12067448size fractionationL6 (Stres-sensitive)durumGSM4630913Water-deficit stress (WS)Rep3
SRR12067447size fractionationL6 (Stres-sensitive)durumGSM4630912Water-deficit stress (WS)Rep2
SRR12067446size fractionationL6 (Stres-sensitive)durumGSM4630911Water-deficit stress (WS)Rep1
SRR12067445size fractionationL6 (Stres-sensitive)durumGSM4630910Control group (CG)Rep3
SRR12067444size fractionationL6 (Stres-sensitive)durumGSM4630909Control group (CG)Rep2
SRR12067443size fractionationL6 (Stres-sensitive)durumGSM4630908Control group (CG)Rep1
SRR12067442size fractionationL6 (Stres-sensitive)durumGSM4630907Water-deficit plus heat stress (WH)Rep3
SRR12067441size fractionationL6 (Stres-sensitive)durumGSM4630906Water-deficit plus heat stress (WH)Rep2
SRR12067440size fractionationL6 (Stres-sensitive)durumGSM4630905Water-deficit plus heat stress (WH)Rep1
SRR12067439size fractionationL6 (Stres-sensitive)durumGSM4630904Heat stress (HS)Rep3
SRR12067438size fractionationL6 (Stres-sensitive)durumGSM4630903Heat stress (HS)Rep2
SRR12067437size fractionationL6 (Stres-sensitive)durumGSM4630902Heat stress (HS)Rep1
SRR12067436size fractionationL6 (Stres-sensitive)durumGSM4630901Water-deficit stress (WS)Rep3
SRR12067435size fractionationL6 (Stres-sensitive)durumGSM4630900Water-deficit stress (WS)Rep2
SRR12067434size fractionationL6 (Stres-sensitive)durumGSM4630899Water-deficit stress (WS)Rep1
SRR12067433size fractionationL6 (Stres-sensitive)durumGSM4630898Control group (CG)Rep3
SRR12067432size fractionationL6 (Stres-sensitive)durumGSM4630897Control group (CG)Rep2
SRR12067431size fractionationL6 (Stres-sensitive)durumGSM4630896Control group (CG)Rep1
SRR12067430size fractionationL6 (Stres-sensitive)durumGSM4630895Water-deficit plus heat stress (WH)Rep3
SRR12067429size fractionationL6 (Stres-sensitive)durumGSM4630894Water-deficit plus heat stress (WH)Rep2
SRR12067428size fractionationL6 (Stres-sensitive)durumGSM4630893Water-deficit plus heat stress (WH)Rep1
SRR12067427size fractionationL6 (Stres-sensitive)durumGSM4630892Heat stress (HS)Rep3
SRR12067426size fractionationL6 (Stres-sensitive)durumGSM4630891Heat stress (HS)Rep2
SRR12067425size fractionationL6 (Stres-sensitive)durumGSM4630890Heat stress (HS)Rep1
SRR12067424size fractionationL6 (Stres-sensitive)durumGSM4630889Water-deficit stress (WS)Rep3
SRR12067423size fractionationL6 (Stres-sensitive)durumGSM4630888Water-deficit stress (WS)Rep2
SRR12067422size fractionationL6 (Stres-sensitive)durumGSM4630887Water-deficit stress (WS)Rep1
SRR12067421size fractionationL6 (Stres-sensitive)durumGSM4630886Control group (CG)Rep3
SRR12067420size fractionationL6 (Stres-sensitive)durumGSM4630885Control group (CG)Rep2
SRR12067419size fractionationL6 (Stres-sensitive)durumGSM4630884Control group (CG)Rep1
SRR12067418size fractionationL6 (Stres-sensitive)durumGSM4630883Water-deficit plus heat stress (WH)Rep3
SRR12067417size fractionationL6 (Stres-sensitive)durumGSM4630882Water-deficit plus heat stress (WH)Rep2
SRR12067416size fractionationL6 (Stres-sensitive)durumGSM4630881Water-deficit plus heat stress (WH)Rep1
SRR12067415size fractionationL6 (Stres-sensitive)durumGSM4630880Heat stress (HS)Rep3
SRR12067414size fractionationL6 (Stres-sensitive)durumGSM4630879Heat stress (HS)Rep2
SRR12067413size fractionationL6 (Stres-sensitive)durumGSM4630878Heat stress (HS)Rep1
SRR12067412size fractionationL6 (Stres-sensitive)durumGSM4630877Water-deficit stress (WS)Rep3
SRR12067411size fractionationL6 (Stres-sensitive)durumGSM4630876Water-deficit stress (WS)Rep2
SRR12067410size fractionationL6 (Stres-sensitive)durumGSM4630875Water-deficit stress (WS)Rep1
SRR12067409size fractionationL6 (Stres-sensitive)durumGSM4630874Control group (CG)Rep3
SRR12067408size fractionationL6 (Stres-sensitive)durumGSM4630873Control group (CG)Rep2
SRR12067407size fractionationL6 (Stres-sensitive)durumGSM4630872Control group (CG)Rep1
SRR12067406size fractionationDBA Aurora (Stress-tolerant)durumGSM4630871Water-deficit plus heat stress (WH)Rep3
SRR12067405size fractionationDBA Aurora (Stress-tolerant)durumGSM4630870Water-deficit plus heat stress (WH)Rep2
SRR12067404size fractionationDBA Aurora (Stress-tolerant)durumGSM4630869Water-deficit plus heat stress (WH)Rep1
SRR12067403size fractionationDBA Aurora (Stress-tolerant)durumGSM4630868Heat stress (HS)Rep3
SRR12067402size fractionationDBA Aurora (Stress-tolerant)durumGSM4630867Heat stress (HS)Rep2
SRR12067401size fractionationDBA Aurora (Stress-tolerant)durumGSM4630866Heat stress (HS)Rep1
SRR12067400size fractionationDBA Aurora (Stress-tolerant)durumGSM4630865Water-deficit stress (WS)Rep3
SRR12067399size fractionationDBA Aurora (Stress-tolerant)durumGSM4630864Water-deficit stress (WS)Rep2
SRR12067398size fractionationDBA Aurora (Stress-tolerant)durumGSM4630863Water-deficit stress (WS)Rep1
SRR12067397size fractionationDBA Aurora (Stress-tolerant)durumGSM4630862Control group (CG)Rep3
SRR12067396size fractionationDBA Aurora (Stress-tolerant)durumGSM4630861Control group (CG)Rep2
SRR12067395size fractionationDBA Aurora (Stress-tolerant)durumGSM4630860Control group (CG)Rep1
SRR12067394size fractionationDBA Aurora (Stress-tolerant)durumGSM4630859Water-deficit plus heat stress (WH)Rep3
SRR12067393size fractionationDBA Aurora (Stress-tolerant)durumGSM4630858Water-deficit plus heat stress (WH)Rep2
SRR12067392size fractionationDBA Aurora (Stress-tolerant)durumGSM4630857Water-deficit plus heat stress (WH)Rep1
SRR12067391size fractionationDBA Aurora (Stress-tolerant)durumGSM4630856Heat stress (HS)Rep3
SRR12067390size fractionationDBA Aurora (Stress-tolerant)durumGSM4630855Heat stress (HS)Rep2
SRR12067389size fractionationDBA Aurora (Stress-tolerant)durumGSM4630854Heat stress (HS)Rep1
SRR12067388size fractionationDBA Aurora (Stress-tolerant)durumGSM4630853Water-deficit stress (WS)Rep3
SRR12067387size fractionationDBA Aurora (Stress-tolerant)durumGSM4630852Water-deficit stress (WS)Rep2
SRR12067386size fractionationDBA Aurora (Stress-tolerant)durumGSM4630851Water-deficit stress (WS)Rep1
SRR12067385size fractionationDBA Aurora (Stress-tolerant)durumGSM4630850Control group (CG)Rep3
SRR12067384size fractionationDBA Aurora (Stress-tolerant)durumGSM4630849Control group (CG)Rep2
SRR12067383size fractionationDBA Aurora (Stress-tolerant)durumGSM4630848Control group (CG)Rep1
SRR12067382size fractionationDBA Aurora (Stress-tolerant)durumGSM4630847Water-deficit plus heat stress (WH)Rep3
SRR12067381size fractionationDBA Aurora (Stress-tolerant)durumGSM4630846Water-deficit plus heat stress (WH)Rep2
SRR12067380size fractionationDBA Aurora (Stress-tolerant)durumGSM4630845Water-deficit plus heat stress (WH)Rep1
SRR12067379size fractionationDBA Aurora (Stress-tolerant)durumGSM4630844Heat stress (HS)Rep3
SRR12067378size fractionationDBA Aurora (Stress-tolerant)durumGSM4630843Heat stress (HS)Rep2
SRR12067377size fractionationDBA Aurora (Stress-tolerant)durumGSM4630842Heat stress (HS)Rep1
SRR12067376size fractionationDBA Aurora (Stress-tolerant)durumGSM4630841Water-deficit stress (WS)Rep3
SRR12067375size fractionationDBA Aurora (Stress-tolerant)durumGSM4630840Water-deficit stress (WS)Rep2
SRR12067374size fractionationDBA Aurora (Stress-tolerant)durumGSM4630839Water-deficit stress (WS)Rep1
SRR12067373size fractionationDBA Aurora (Stress-tolerant)durumGSM4630838Control group (CG)Rep3
SRR12067372size fractionationDBA Aurora (Stress-tolerant)durumGSM4630837Control group (CG)Rep2
SRR12067371size fractionationDBA Aurora (Stress-tolerant)durumGSM4630836Control group (CG)Rep1
SRR12067370size fractionationDBA Aurora (Stress-tolerant)durumGSM4630835Water-deficit plus heat stress (WH)Rep3
SRR12067369size fractionationDBA Aurora (Stress-tolerant)durumGSM4630834Water-deficit plus heat stress (WH)Rep2
SRR12067368size fractionationDBA Aurora (Stress-tolerant)durumGSM4630833Water-deficit plus heat stress (WH)Rep1
SRR12067367size fractionationDBA Aurora (Stress-tolerant)durumGSM4630832Heat stress (HS)Rep3
SRR12067366size fractionationDBA Aurora (Stress-tolerant)durumGSM4630831Heat stress (HS)Rep2
SRR12067365size fractionationDBA Aurora (Stress-tolerant)durumGSM4630830Heat stress (HS)Rep1
SRR12067364size fractionationDBA Aurora (Stress-tolerant)durumGSM4630829Water-deficit stress (WS)Rep3
SRR12067363size fractionationDBA Aurora (Stress-tolerant)durumGSM4630828Water-deficit stress (WS)Rep2
SRR12067362size fractionationDBA Aurora (Stress-tolerant)durumGSM4630827Water-deficit stress (WS)Rep1
SRR12067361size fractionationDBA Aurora (Stress-tolerant)durumGSM4630826Control group (CG)Rep3
SRR12067360size fractionationDBA Aurora (Stress-tolerant)durumGSM4630825Control group (CG)Rep2
SRR12067359size fractionationDBA Aurora (Stress-tolerant)durumGSM4630824Control group (CG)Rep1
SRR12067358size fractionationDBA Aurora (Stress-tolerant)durumGSM4630823Water-deficit plus heat stress (WH)Rep3
SRR12067357size fractionationDBA Aurora (Stress-tolerant)durumGSM4630822Water-deficit plus heat stress (WH)Rep2
SRR12067356size fractionationDBA Aurora (Stress-tolerant)durumGSM4630821Water-deficit plus heat stress (WH)Rep1
SRR12067355size fractionationDBA Aurora (Stress-tolerant)durumGSM4630820Heat stress (HS)Rep3
SRR12067354size fractionationDBA Aurora (Stress-tolerant)durumGSM4630819Heat stress (HS)Rep2
SRR12067353size fractionationDBA Aurora (Stress-tolerant)durumGSM4630818Heat stress (HS)Rep1
SRR12067352size fractionationDBA Aurora (Stress-tolerant)durumGSM4630817Water-deficit stress (WS)Rep3
SRR12067351size fractionationDBA Aurora (Stress-tolerant)durumGSM4630816Water-deficit stress (WS)Rep2
SRR12067350size fractionationDBA Aurora (Stress-tolerant)durumGSM4630815Water-deficit stress (WS)Rep1
SRR12067349size fractionationDBA Aurora (Stress-tolerant)durumGSM4630814Control group (CG)Rep3
SRR12067348size fractionationDBA Aurora (Stress-tolerant)durumGSM4630813Control group (CG)Rep2
SRR12067347size fractionationDBA Aurora (Stress-tolerant)durumGSM4630812Control group (CG)Rep1

Run LibrarySelection Genotype Tissue sub_species Sample Name Treatment
SRR10870722size fractionationLine 6 (UAD1301020-8)shootdurumGSM4259761Water-deficit and heat stress
SRR10870721size fractionationLine 6 (UAD1301020-8)shootdurumGSM4259760Control
SRR10870720size fractionationDBA AurorashootdurumGSM4259759Water-deficit and heat stress
SRR10870719size fractionationDBA AurorashootdurumGSM4259758Control

Run Library Name LibrarySelection Tissue sub_species Cultivar Sample Name geo_loc_name Treatment
SRR7226493svevomicro9RT-PCRGraindurumSvevoSvevo_miRNA_9Italy-
SRR7226494svevomicro7RT-PCRRachis + glumes Leaf Epicotile + shoot Shoot RootletdurumSvevoSvevo_miRNA_7ItalyF. graminearum and F. culmorum inoculated spikes 36/72h or Zymoseptoria tritici Italian isolate inoculated seedlings 4h /1/2/9 d or Control spikes dd H2O 36/72h or control leaves ddH2O 4h / 1/2/9 d
SRR7226495svevomicro8RT-PCRShoot Root LeafdurumSvevoSvevo_miRNA_8Italy1/4 Hoagland solution (HS) + 5mM KNO3 4/24/72 h / 7 d or 1/4 HS + K(H2PO4) 2mM 4/24/72 h / 7 d or 1/4 HS + 2.5 mM Mg(SO4) 4/24/72 h / 7 d or 1/4 HS + 100 µM Fe-EDTA 4/24/72 h / 7 d or 1/4 HS + 10mM (NH4)NO3 4/24/72 h / 7 d
SRR7226496svevomicro6RT-PCRColeoptile Root Culm node Spike Flag Leaf Glume + lemna Ovary + antherdurumSvevoSvevo_miRNA_6Italy-
SRR7226499svevomicro5RT-PCRLeaf RootdurumSvevoSvevo_miRNA_5Italy60µM CdCl2/2 d or 10µM ABA/4h or 50µM GA3/4h
SRR7226500svevomicro3RT-PCRLeaf RootdurumSvevoSvevo_miRNA_3Italy25°C /20°C 12h light/12h dark or Nutrient supply/control: control 1/4 strength Hoagland solution then replenished with dd H2O
SRR7226498svevomicro4RT-PCRLeaf RootdurumSvevoSvevo_miRNA_4Italy1/4 Hoagland solution + K(H2PO4) 2mM 4/24/72 h / 7 d or 1/4 Hoagland solution + 10mM (NH4)NO3 4/24/72 h / 7 d or UV exposure: 3h first day/2h second day/2h third day + recovery 24h or 10mM H2O2 2/4h
SRR7226501svevomicro1RT-PCRLeaf Root Coleoptyle SpikedurumSvevoSvevo_miRNA_1Italy3°C/1.5°C 12h light /12h dark /24 h or 36°C/ 4h or 37°C/24h ,37°C/24h + 45°C/1h, 37°C/24h + 45°C/1h + 24°C/3d
SRR7226497svevomicro2RT-PCRLeaf RootdurumSvevoSvevo_miRNA_2ItalyDrought/wounding 90 min/3h or 20% PEG 6000 3h/7h or 150mM NaCl 6h/24h

Run Library Name LibrarySelection Stage Tissue sub_species Cultivar Sample Name geo_loc_name Treatment
SRR6649861LIB_02size fractionationseedilingleavesdurumCiccioLIB02_Ciccio_F0_sRNAItalyNitrogen deprivation
SRR6649862LIB_01size fractionationseedilingrootsdurumCiccioLIB01_Ciccio_R0_sRNAItalyNitrogen deprivation
SRR6649863LIB_04size fractionationseedilingleavesdurumCiccioLIB04_Ciccio_F2_sRNAItaly-
SRR6649864LIB_03size fractionationseedilingrootsdurumCiccioLIB03_Ciccio_R2_sRNAItaly-
SRR6649865LIB_06size fractionationseedilingleavesdurumSvevoLIB06_Svevo_F0_sRNAItalyNitrogen deprivation
SRR6649866LIB_05size fractionationseedilingrootsdurumSvevoLIB05_Svevo_R0_sRNAItalyNitrogen deprivation
SRR6649867LIB_08size fractionationseedilingleavesdurumSvevoLIB08_Svevo_F2_sRNAItaly-
SRR6649868LIB_07size fractionationseedilingrootsdurumSvevoLIB07_Svevo_R2_sRNAItaly-
SRR3607662LIB_11size fractionationlate milkroot,leaf,flag leaf,spikedurumSvevoLIBRARY 11 smallRNA from Triticum turgidum subsp. durum - Svevo (0 mM N)ItalyNitrogen deprivation
SRR3603231LIB_09size fractionationlate milkroot,leaf,flag leaf,spikedurumCiccioLIBRARY 09 smallRNA from Triticum turgidum subsp. durum - Ciccio (0 mM N)ItalyNitrogen deprivation
SRR3147015PRJNA310686size fractionationLate milk - Z77small RNA pool from roots,leaves,flag leaves,spikesdurumSvevoSmall RNA durum wheat LIBRARY 12Italy-
SRR3144594Library 10 durum wheat CiccioRANDOMLate milk - Z77small RNA pool from roots,leaves,flag leaves,spikesdurumCiccioSmall RNA durum wheat LIBRARY 10Italy-

Experiment Accession Run LibrarySelection Tissue sub_species variety_name Sample Name Treatment
SRX1042025SRR2043911size fractionationDeveloping head (H)durumTjilkuriGSM1698216Water deficit stress treatment group (WG)
SRX1042024SRR2043909 SRR2043910size fractionationDeveloping head (H)durumTjilkuriGSM1698215Water deficit stress treatment group (WG)
SRX1042023SRR2043907 SRR2043908size fractionationDeveloping head (H)durumTjilkuriGSM1698214Water deficit stress treatment group (WG)
SRX1042022SRR2043905 SRR2043906size fractionationDeveloping head (H)durumTjilkuriGSM1698213Water deficit stress treatment group (WG)
SRX1042021SRR2043903 SRR2043904size fractionationDeveloping head (H)durumTjilkuriGSM1698212Water deficit stress treatment group (WG)
SRX1042020SRR2043901 SRR2043902size fractionationDeveloping head (H)durumTjilkuriGSM1698211Water deficit stress treatment group (WG)
SRX1042019SRR2043899 SRR2043900size fractionationDeveloping head (H)durumTjilkuriGSM1698210Control Group (CG)
SRX1042018SRR2043897 SRR2043898size fractionationDeveloping head (H)durumTjilkuriGSM1698209Control Group (CG)
SRX1042017SRR2043895 SRR2043896size fractionationDeveloping head (H)durumTjilkuriGSM1698208Control Group (CG)
SRX1042016SRR2043893 SRR2043894size fractionationDeveloping head (H)durumTjilkuriGSM1698207Control Group (CG)
SRX1042015SRR2043892size fractionationDeveloping head (H)durumTjilkuriGSM1698206Control Group (CG)
SRX1042014SRR2043890 SRR2043891size fractionationDeveloping head (H)durumTjilkuriGSM1698205Control Group (CG)
SRX1042013SRR2043888 SRR2043889size fractionationFlag leaf (FL)durumTjilkuriGSM1698204Water deficit stress treatment group (WG)
SRX1042012SRR2043887size fractionationFlag leaf (FL)durumTjilkuriGSM1698203Water deficit stress treatment group (WG)
SRX1042011SRR2043885 SRR2043886size fractionationFlag leaf (FL)durumTjilkuriGSM1698202Water deficit stress treatment group (WG)
SRX1042010SRR2043883 SRR2043884size fractionationFlag leaf (FL)durumTjilkuriGSM1698201Water deficit stress treatment group (WG)
SRX1042009SRR2043881 SRR2043882size fractionationFlag leaf (FL)durumTjilkuriGSM1698200Water deficit stress treatment group (WG)
SRX1042008SRR2043879 SRR2043880size fractionationFlag leaf (FL)durumTjilkuriGSM1698199Water deficit stress treatment group (WG)
SRX1042007SRR2043877 SRR2043878size fractionationFlag leaf (FL)durumTjilkuriGSM1698198Control Group (CG)
SRX1042006SRR2043875 SRR2043876size fractionationFlag leaf (FL)durumTjilkuriGSM1698197Control Group (CG)
SRX1042005SRR2043873 SRR2043874size fractionationFlag leaf (FL)durumTjilkuriGSM1698196Control Group (CG)
SRX1042004SRR2043872size fractionationFlag leaf (FL)durumTjilkuriGSM1698195Control Group (CG)
SRX1042003SRR2043871size fractionationFlag leaf (FL)durumTjilkuriGSM1698194Control Group (CG)
SRX1042002SRR2043869 SRR2043870size fractionationFlag leaf (FL)durumTjilkuriGSM1698193Control Group (CG)
SRX1042001SRR2043867 SRR2043868size fractionationDeveloping head (H)durumYawaGSM1698192Water deficit stress treatment group (WG)
SRX1042000SRR2043865 SRR2043866size fractionationDeveloping head (H)durumYawaGSM1698191Water deficit stress treatment group (WG)
SRX1041999SRR2043863 SRR2043864size fractionationDeveloping head (H)durumYawaGSM1698190Water deficit stress treatment group (WG)
SRX1041998SRR2043862size fractionationDeveloping head (H)durumYawaGSM1698189Water deficit stress treatment group (WG)
SRX1041997SRR2043860 SRR2043861size fractionationDeveloping head (H)durumYawaGSM1698188Water deficit stress treatment group (WG)
SRX1041996SRR2043858 SRR2043859size fractionationDeveloping head (H)durumYawaGSM1698187Water deficit stress treatment group (WG)
SRX1041995SRR2043857size fractionationDeveloping head (H)durumYawaGSM1698186Control Group (CG)
SRX1041994SRR2043855 SRR2043856size fractionationDeveloping head (H)durumYawaGSM1698185Control Group (CG)
SRX1041993SRR2043853 SRR2043854size fractionationDeveloping head (H)durumYawaGSM1698184Control Group (CG)
SRX1041992SRR2043851 SRR2043852size fractionationDeveloping head (H)durumYawaGSM1698183Control Group (CG)
SRX1041991SRR2043849 SRR2043850size fractionationDeveloping head (H)durumYawaGSM1698182Control Group (CG)
SRX1041990SRR2043847 SRR2043848size fractionationDeveloping head (H)durumYawaGSM1698181Control Group (CG)
SRX1041989SRR2043845 SRR2043846size fractionationFlag leaf (FL)durumYawaGSM1698180Water deficit stress treatment group (WG)
SRX1041988SRR2043843 SRR2043844size fractionationFlag leaf (FL)durumYawaGSM1698179Water deficit stress treatment group (WG)
SRX1041987SRR2043841 SRR2043842size fractionationFlag leaf (FL)durumYawaGSM1698178Water deficit stress treatment group (WG)
SRX1041986SRR2043839 SRR2043840size fractionationFlag leaf (FL)durumYawaGSM1698177Water deficit stress treatment group (WG)
SRX1041985SRR2043837 SRR2043838size fractionationFlag leaf (FL)durumYawaGSM1698176Water deficit stress treatment group (WG)
SRX1041984SRR2043835 SRR2043836size fractionationFlag leaf (FL)durumYawaGSM1698175Water deficit stress treatment group (WG)
SRX1041983SRR2043833 SRR2043834size fractionationFlag leaf (FL)durumYawaGSM1698174Control Group (CG)
SRX1041982SRR2043831 SRR2043832size fractionationFlag leaf (FL)durumYawaGSM1698173Control Group (CG)
SRX1041981SRR2043830size fractionationFlag leaf (FL)durumYawaGSM1698172Control Group (CG)
SRX1041980SRR2043828 SRR2043829size fractionationFlag leaf (FL)durumYawaGSM1698171Control Group (CG)
SRX1041979SRR2043826 SRR2043827size fractionationFlag leaf (FL)durumYawaGSM1698170Control Group (CG)
SRX1041978SRR2043825size fractionationFlag leaf (FL)durumYawaGSM1698169Control Group (CG)
SRX1041977SRR2043823 SRR2043824size fractionationDeveloping head (H)durumEGA BellaroiGSM1698168Water deficit stress treatment group (WG)
SRX1041976SRR2043821 SRR2043822size fractionationDeveloping head (H)durumEGA BellaroiGSM1698167Water deficit stress treatment group (WG)
SRX1041975SRR2043819 SRR2043820size fractionationDeveloping head (H)durumEGA BellaroiGSM1698166Water deficit stress treatment group (WG)
SRX1041974SRR2043818size fractionationDeveloping head (H)durumEGA BellaroiGSM1698165Water deficit stress treatment group (WG)
SRX1041973SRR2043816 SRR2043817size fractionationDeveloping head (H)durumEGA BellaroiGSM1698164Water deficit stress treatment group (WG)
SRX1041972SRR2043814 SRR2043815size fractionationDeveloping head (H)durumEGA BellaroiGSM1698163Water deficit stress treatment group (WG)
SRX1041971SRR2043813size fractionationDeveloping head (H)durumEGA BellaroiGSM1698162Control Group (CG)
SRX1041970SRR2043811 SRR2043812size fractionationDeveloping head (H)durumEGA BellaroiGSM1698161Control Group (CG)
SRX1041969SRR2043809 SRR2043810size fractionationDeveloping head (H)durumEGA BellaroiGSM1698160Control Group (CG)
SRX1041968SRR2043807 SRR2043808size fractionationDeveloping head (H)durumEGA BellaroiGSM1698159Control Group (CG)
SRX1041967SRR2043805 SRR2043806size fractionationDeveloping head (H)durumEGA BellaroiGSM1698158Control Group (CG)
SRX1041966SRR2043803 SRR2043804size fractionationDeveloping head (H)durumEGA BellaroiGSM1698157Control Group (CG)
SRX1041965SRR2043801 SRR2043802size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698156Water deficit stress treatment group (WG)
SRX1041964SRR2043800size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698155Water deficit stress treatment group (WG)
SRX1041963SRR2043799size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698154Water deficit stress treatment group (WG)
SRX1041962SRR2043797 SRR2043798size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698153Water deficit stress treatment group (WG)
SRX1041961SRR2043796size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698152Water deficit stress treatment group (WG)
SRX1041960SRR2043794 SRR2043795size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698151Water deficit stress treatment group (WG)
SRX1041959SRR2043793size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698150Control Group (CG)
SRX1041958SRR2043791 SRR2043792size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698149Control Group (CG)
SRX1041957SRR2043789 SRR2043790size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698148Control Group (CG)
SRX1041956SRR2043788size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698147Control Group (CG)
SRX1041955SRR2043787size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698146Control Group (CG)
SRX1041954SRR2043786size fractionationFlag leaf (FL)durumEGA BellaroiGSM1698145Control Group (CG)
SRX1041953SRR2043784 SRR2043785size fractionationDeveloping head (H)durumTamaroiGSM1698144Water deficit stress treatment group (WG)
SRX1041952SRR2043782 SRR2043783size fractionationDeveloping head (H)durumTamaroiGSM1698143Water deficit stress treatment group (WG)
SRX1041951SRR2043780 SRR2043781size fractionationDeveloping head (H)durumTamaroiGSM1698142Water deficit stress treatment group (WG)
SRX1041950SRR2043779size fractionationDeveloping head (H)durumTamaroiGSM1698141Water deficit stress treatment group (WG)
SRX1041949SRR2043778size fractionationDeveloping head (H)durumTamaroiGSM1698140Water deficit stress treatment group (WG)
SRX1041948SRR2043776 SRR2043777size fractionationDeveloping head (H)durumTamaroiGSM1698139Water deficit stress treatment group (WG)
SRX1041947SRR2043775size fractionationDeveloping head (H)durumTamaroiGSM1698138Control Group (CG)
SRX1041946SRR2043773 SRR2043774size fractionationDeveloping head (H)durumTamaroiGSM1698137Control Group (CG)
SRX1041945SRR2043771 SRR2043772size fractionationDeveloping head (H)durumTamaroiGSM1698136Control Group (CG)
SRX1041944SRR2043769 SRR2043770size fractionationDeveloping head (H)durumTamaroiGSM1698135Control Group (CG)
SRX1041943SRR2043767 SRR2043768size fractionationDeveloping head (H)durumTamaroiGSM1698134Control Group (CG)
SRX1041942SRR2043766size fractionationDeveloping head (H)durumTamaroiGSM1698133Control Group (CG)
SRX1041941SRR2043765size fractionationFlag leaf (FL)durumTamaroiGSM1698132Water deficit stress treatment group (WG)
SRX1041940SRR2043763 SRR2043764size fractionationFlag leaf (FL)durumTamaroiGSM1698131Water deficit stress treatment group (WG)
SRX1041939SRR2043761 SRR2043762size fractionationFlag leaf (FL)durumTamaroiGSM1698130Water deficit stress treatment group (WG)
SRX1041938SRR2043759 SRR2043760size fractionationFlag leaf (FL)durumTamaroiGSM1698129Water deficit stress treatment group (WG)
SRX1041937SRR2043757 SRR2043758size fractionationFlag leaf (FL)durumTamaroiGSM1698128Water deficit stress treatment group (WG)
SRX1041936SRR2043755 SRR2043756size fractionationFlag leaf (FL)durumTamaroiGSM1698127Water deficit stress treatment group (WG)
SRX1041935SRR2043754size fractionationFlag leaf (FL)durumTamaroiGSM1698126Control Group (CG)
SRX1041934SRR2043752 SRR2043753size fractionationFlag leaf (FL)durumTamaroiGSM1698125Control Group (CG)
SRX1041933SRR2043750 SRR2043751size fractionationFlag leaf (FL)durumTamaroiGSM1698124Control Group (CG)
SRX1041932SRR2043748 SRR2043749size fractionationFlag leaf (FL)durumTamaroiGSM1698123Control Group (CG)
SRX1041931SRR2043746 SRR2043747size fractionationFlag leaf (FL)durumTamaroiGSM1698122Control Group (CG)
SRX1041930SRR2043745size fractionationFlag leaf (FL)durumTamaroiGSM1698121Control Group (CG)

Run LibrarySelection Stage Tissue Cultivar Sample Name geo_loc_name
SRR5460520RT-PCR6 days after pollinationendospermTMU06TMU06(AA)_endosperm_sRNAChina:Nanjing
SRR5460519RT-PCR6 days after pollinationendospermTMU06TMU06(AA)_endosperm_sRNAChina:Nanjing
SRR5460518RT-PCR6 days after pollinationendospermTMU06TMU06(AA)_endosperm_sRNAChina:Nanjing
SRR5460508RT-PCR6 days after pollinationendospermTMU38TMU38(AA)_endosperm_sRNAChina:Nanjing
SRR5460507RT-PCR6 days after pollinationendospermTMU38TMU38(AA)_endosperm_sRNAChina:Nanjing
SRR5460481RT-PCR6 days after pollinationendospermTMU38TMU38(AA)_endosperm_sRNAChina:Nanjing
SRR5460523RT-PCR6 days after pollinationendospermTL05TL05(SS)_endosperm_sRNAChina:Nanjing
SRR5460522RT-PCR6 days after pollinationendospermTL05TL05(SS)_endosperm_sRNAChina:Nanjing
SRR5460521RT-PCR6 days after pollinationendospermTL05TL05(SS)_endosperm_sRNAChina:Nanjing
SRR5460513RT-PCR6 days after pollinationendospermTQ27TQ27(DD)_endosperm_sRNAChina:Nanjing
SRR5460512RT-PCR6 days after pollinationendospermTQ27TQ27(DD)_endosperm_sRNAChina:Nanjing
SRR5460509RT-PCR6 days after pollinationendospermTQ27TQ27(DD)_endosperm_sRNAChina:Nanjing
SRR5460516RT-PCR6 days after pollinationendospermAT2AT2(AADD)_endosperm_sRNAChina:Nanjing
SRR5460517RT-PCR6 days after pollinationendospermAT2AT2(AADD)_endosperm_sRNAChina:Nanjing
SRR5460524RT-PCR6 days after pollinationendospermTLTUTLTU(SSAA)_endosperm_sRNAChina:Nanjing
SRR5460526RT-PCR6 days after pollinationendospermTLTUTLTU(SSAA)_endosperm_sRNAChina:Nanjing
SRR5460528RT-PCR6 days after pollinationendospermTLTUTLTU(SSAA)_endosperm_sRNAChina:Nanjing
SRR5460515RT-PCR6 days after pollinationendospermAT2AT2(AADD)_endosperm_sRNAChina:Nanjing

Run Library Name LibrarySelection Ecotype Stage Tissue Sample Name geo_loc_name Treatment
SRR22878362AGA-sRNAPCRGGAAxAA-F1three-leafseedlingSample 1Chinasmall RNA
SRR22878361AGD-sRNAPCRGGAAxDD-F1three-leafseedlingSample 2Chinasmall RNA

Run LibrarySource LibrarySelection Age Tissue Strain Phenotype Sample Name geo_loc_name
DRR057233TRANSCRIPTOMICsize fractionation8-weekscrownLdn/KU-2059normalSAMD00048852Japan:Kobe
DRR057234TRANSCRIPTOMICsize fractionation8 weekscrownLdn/KU-2025type II necrosisSAMD00048853Japan:Kobe
DRR057235TRANSCRIPTOMICsize fractionation8-weekscrownLdn/KU-2059normalSAMD00048854Japan:Kobe
DRR057236TRANSCRIPTOMICsize fractionation8-weekscrownLdn/KU-2025type II necrosisSAMD00048855Japan:Kobe

Run Library Name LibrarySelection Age Tissue Sample Name geo_loc_name
SRR15041375D_48h1othertrifoliate stageleafD_48h1China:Inner Mongolia, Hohhot
SRR15041376D_24h3othertrifoliate stageleafD_24h3China:Inner Mongolia, Hohhot
SRR15041377D_24h2othertrifoliate stageleafD_24h2China:Inner Mongolia, Hohhot
SRR15041378D_24h1othertrifoliate stageleafD_24h1China:Inner Mongolia, Hohhot
SRR15041379D_12h3othertrifoliate stageleafD_12h3China:Inner Mongolia, Hohhot
SRR15041380D_12h2othertrifoliate stageleafD_12h2China:Inner Mongolia, Hohhot
SRR15041381D_12h1othertrifoliate stageleafD_12h1China:Inner Mongolia, Hohhot
SRR15041382R_24h3othertrifoliate stageleafR_24h3China:Inner Mongolia, Hohhot
SRR15041383R_24h2othertrifoliate stageleafR_24h2China:Inner Mongolia, Hohhot
SRR15041384R_24h1othertrifoliate stageleafR_24h1China:Inner Mongolia, Hohhot
SRR15041385D_7d3othertrifoliate stageleafD_7d3China:Inner Mongolia, Hohhot
SRR15041386CK_3othertrifoliate stageleafCK_3China:Inner Mongolia, Hohhot
SRR15041387D_7d2othertrifoliate stageleafD_7d2China:Inner Mongolia, Hohhot
SRR15041388D_7d1othertrifoliate stageleafD_7d1China:Inner Mongolia, Hohhot
SRR15041389D_5d3othertrifoliate stageleafD_5d3China:Inner Mongolia, Hohhot
SRR15041390D_5d2othertrifoliate stageleafD_5d2China:Inner Mongolia, Hohhot
SRR15041391D_5d1othertrifoliate stageleafD_5d1China:Inner Mongolia, Hohhot
SRR15041392D_3d3othertrifoliate stageleafD_3d3China:Inner Mongolia, Hohhot
SRR15041393D_3d2othertrifoliate stageleafD_3d2China:Inner Mongolia, Hohhot
SRR15041394D_3d1othertrifoliate stageleafD_3d1China:Inner Mongolia, Hohhot
SRR15041395D_48h3othertrifoliate stageleafD_48h3China:Inner Mongolia, Hohhot
SRR15041396D_48h2othertrifoliate stageleafD_48h2China:Inner Mongolia, Hohhot
SRR15041397CK_2othertrifoliate stageleafCK_2China:Inner Mongolia, Hohhot
SRR15041398CK_1othertrifoliate stageleafCK_1China:Inner Mongolia, Hohhot

Run Library Name LibrarySelection Tissue Sample Name geo_loc_name
SRR6824284S02size fractionationleavesmirna from Agropyron mongolicum under drought stressChina:InnerMongolia
SRR6516044S01-leavesAgropyron mongolicum Keng mirnaChina:InnerMongolia
SRR6516713S01size fractionationleavesAgropyron mongolicum Keng mirnaChina:InnerMongolia
SRR6516045S02size fractionationleavesmirna from Agropyron mongolicum under drought stressChina:InnerMongolia

Run Library Name LibrarySelection Stage Tissue Sample Name geo_loc_name
SRR9021153Psathyrostachys shuashanica-mock-2size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021154Psathyrostachys shuashanica-mock-3size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021155Psathyrostachys shuashanica-14d-3size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021156Psathyrostachys shuashanica-mock-1size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021157Psathyrostachys shuashanica-14d-1size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021158Psathyrostachys shuashanica-14d-2size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021159Psathyrostachys shuashanica-7d-2size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021160Psathyrostachys shuashanica-7d-3size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021161Psathyrostachys shuashanica-3d-3size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021162Psathyrostachys shuashanica-7d-1size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021163Psathyrostachys shuashanica-3d-1size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi
SRR9021164Psathyrostachys shuashanica-3d-2size fractionationseedling stageleafPsathyrostachys huashanica-leafChina:shaanxi