Triticum aestivum

BioProject Information

Project ID Title Publication Instrument Description Notes
PRJNA341486small RNA and RNA profiling in Triticum turgidum and Triticum aestivum-Illumina HiSeq 2500Identification of key genes for wax production.Key gene
PRJNA551738The miRNA-seq of three tissues for Guomai 301 (Triticum aestivum)Gene Expression Profiles and microRNA Regulation Networks in Tiller Primordia, Stem Tips, and Young Spikes of Wheat Guomai 301Illumina HiSeq X TenTo find gene expression profiles of tiller primordia, stem tips and young spikes for Guomai 301 (Triticum aestivum), we carried out the miRNA-seq.Gene expression
PRJNA642367The impacts of m6A methylation on mRNA translation of wheatTranscriptome-wide analyses of RNA m6A methylation in hexaploid wheat reveal its roles in mRNA translation regulationIllumina HiSeq 2500To investigate the roles of m6A methylation in the regulation of mRNA translation of wheat genes. We conducted transcriptome profiling of m6A and small RNA, and measured the translation levels of mRNAs in wheat spikes. The translation efficienies were compared between mRNAs with or wihtout m6A.RNA m6A methylation
PRJNA241290Triticum aestivum strain:Chinese spring Transcriptome or Gene expressionWhole-genome discovery of miRNAs and their targets in wheat (Triticum aestivum L.)Illumina Genome Analyzer IIxTo identify as much wheat miRNAs and their targets.Wheat miRNAs and their targets
PRJNA275100Expression profiling of small RNAs in developing wheat grainsSmall RNA and Degradome Sequencing Reveal Complex Roles of miRNAs and Their Targets in Developing Wheat GrainsIllumina HiSeq 2000We aimed to identify differentially expressed miRNAs during wheat grain development by using high-throughput sequencing approach. Four small RNA libraries were constructed from wheat grains collected at 7, 14, 21 and 28 days post anthesis (DPA). A total of 165 known miRNAs and 37 novel miRNAs were identified in four small RNA libraries. Moreover, a miRNA-like long hairpin locus was first identified to produce 21~22-nt phased siRNAs. A comparison of the miRNAomes revealed that 55 miRNA families were differentially expressed during the grain development. Overall design: Examination of 4 different small RNA expression profilings in the 4 developmental stages of wheat grains.7, 14, 21 and 28 days post anthesis (DPA)
PRJNA309061Triticum aestivum Raw sequence readsComparison of small RNA next-generation sequencing with and without isolation of small RNA fractionIllumina HiScanSQThe aim of the present study was to compare data obtained by sequencing small libraries prepared from isolated small RNA fraction and from total RNA.Isolated small RNA fraction and total RNA
PRJNA309881Triticum aestivum Transcriptome or Gene expressionIdentification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro CultureIllumina HiSeq 2000wheat callus miRNA.Mature and Immature Embryos
PRJNA383333Micro-RNA sequencing of four different stages of spike in wheatTranscriptome Profiling of Wheat Inflorescence Development from Spikelet Initiation to Floral Patterning Identified Stage-Specific Regulatory GenesIllumina HiSeq 2000Micro-RNA sequencing of four different stages (DR,FM,AM,TS) of spike in wheat.Four different stages (DR,FM,AM,TS)
PRJNA516200Identification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the FieldIdentification and Temporal Expression Analysis of Conserved and Novel MicroRNAs in the Leaves of Winter Wheat Grown in the FieldBGISEQ-500MicroRNAs (miRNAs) are small non-coding RNAs that regulate target mRNAs by inducing degradation or preventing translation of their target mRNAs. Winter wheat, Triticum aestivum., is an important crop plant, yet there are only a few studies on the association of miRNAs and growth and development of winter wheat grown in the field. Here we carried out experimental analysis of miRNAs in wheat leaves by analyzing small RNA profiles at different growth stages. Overall design: To identify miRNAs involved in winter wheat growth and development, we performed high-throughput sequencing of sRNA libraries from leaves at 4 different growth and development stages: three leaf stage, winter dormancy stage, spring greenup stage and jointing stage.Winter wheat cultivar Shimai 22 was grown in the field under natural weather condition;BGISEQ500 RS (BGI, China)platform was used.Three leaf stage, winter dormancy stage, spring greenup stage and jointing stage
PRJNA636099sRNA and RNA sequencing profile of barley and wheat antherPremeiotic, 24-Nucleotide Reproductive PhasiRNAs Are Abundant in Anthers of Wheat and Barley But Not Rice and MaizeNextSeq 550Two classes of pre-meiotic (21-nt) and meiotic (24-nt) phasiRNAs and their patterns of accumulation, have been described in maize and rice anthers. Their precise function remains unclear but some studies have shown that they support male fertility. Their important role in anthers underpins our current study to characterize phasiRNAs in wheat and barley anthers. We staged anthers at every 0.2 mm of development for one wheat and two barley varieties. We identified pre-meiotic (0.2 mm, 0.4 mm and 0.6 mm), meiotic (0.8 mm, 1.0 mm and 1.4 mm) and post-meiotic (1.8 mm) anthers for which we then investigated accumulation patterns of RNAs, including reproductive phasiRNAs.Pre-meiotic、post-meiotic and reproductive
PRJNA656372Temporal expression study of microRNAs in crown tissues of winter wheat grown under natural growth conditionTemporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditionsBGISEQ-500MicroRNAs (miRNAs) are single strand small non-coding RNAs that regulate target mRNAs at post-transcription level. Winter wheat (Triticum aestivum L.), is an important crop plant all over the world. Long term cold exposure (vernalization) is necessary for winter wheat transition from vegetative growth to reproductive growth, yet the involvement of miRNAs in these stages remains unknown. Therefore, we performed next generation sequencing of small RNAs profiles in crown tissues at three-leaf stage, winter dormancy stage, spring greenup stage and jointing stage. Overall design: To identify miRNAs involved in winter wheat growth and development in te field, winter wheat cultivar Shimai 22 was grown in the field under natural weather condition. Crown tissues were collected at 4 different growth and development stages: three leaf stage, winter dormancy stage, spring greenup stage and jointing stage, small RNA libraries were constructed, and BGISEQ500 RS (BGI, China) sequencing platform was used for small RNA libraries sequencing.Three leaf stage, winter dormancy stage, spring greenup stage and jointing stage

Project ID Title Publication Instrument Description Notes
PRJNA171754Triticum aestivum strain:HD2985, HD2329 Transcriptome or Gene expressionIdentification of Putative RuBisCo Activase (TaRca1)—The Catalytic Chaperone Regulating Carbon Assimilatory Pathway in Wheat (Triticum aestivum) under the Heat StressIllumina HiSeq 2000The study was conducted in order to find out the differential change in the transcript of tolerant and susceptible wheat cultivar under heat stress and to decipher the mechanism of thermotolerance in wheat by identifying novel genes and transcription factors involved in the pathways. Wheat cultivar HD2985 (thermotolerant) and HD2329 (thermosusceptible) were exposed to heat stress of 42 degree for 4h at pollination stage and samples were collected from both control and heat shock treated plants for further characterization.Heat stress
PRJNA284172Triticum aestivum cultivar:HD-2967 Transcriptome or Gene expressionMolecular Characterization of GS2 and Fd-GOGAT Homeologues and Their Biased Response to Nitrogen Stress in Bread Wheat (Triticum aestivum L.)Illumina HiSeq 2500microRNAs from root and shoot tissues of wheat. Submitted by: Subodh K. Sinha and P. K. Mandal.Root and shoot tissues of wheat
PRJNA867659Analysis of small RNA in wheat ovary stages infected by Tilletia laevis KuhnCharacteristics of the Infection of Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) in Compatible WheatIllumina HiSeq 2500We used a high-throughput sequencing approach to determine miRNAs in three different developmental stages of T. laevis-infected wheat ovaries.Wheat ovary stage
PRJNA480952Small RNA sequncing of Wheat infected by Zymoseptoria tritici during the infection cycleSmall RNA Bidirectional Crosstalk During the Interaction Between Wheat and Zymoseptoria triticiNextSeq 500We studied the bidirectional cross-kingdom RNAi in wheat-Z. tritici pathosystem. The whole dataset contains sRNA-seq, degradome sequencing and mRNA-seq. The sRNA-seq samples include wheat infected by Z. tritici at 7dpi, 12dpi and 14dpi, wheat without infections (Mocks) at 7dpi, 12dpi and 14dpi, and Z. tritici grow in vitro. All of the sRNA-seq samples have three replicates. The degradome sequencing data include mocks (7dpi and 12dpi), wheat infected by Z. tritici (7dpi and 12dpi) and Z. tritici grow in vitro. Additionally, there are as well mRNA-seq of mocks at 7dpi and 12dpi, which have three replicates.7dpi, 12dpi and 14dpi
PRJNA266709Triticum aestivum cultivar:HD2329Uncovering leaf rust responsive miRNAs in wheat (Triticum aestivum L.) using high-throughput sequencing and prediction of their targets through degradome analysisIllumina MiSeqThe major threat to wheat production in past decades has been the rust epidemics. The present study was targeted towards understanding of the mechanism and functional characterization of miRNAs of wheat in response to leaf rust ingression. With the improvement in methods to explore the transcriptome, in recent years there have been great advances in identifying and understanding non-coding RNAs. Recent studies indicate involvements of miRNAs in plant development, growth and responses to biotic and abiotic stresses, adaptive responses, metabolism, and signal transduction. Therefore, Illumina’s deep sequencing technology was used for transcriptome-wide identification of miRNAs and their expression profiling in response to leaf rust infection using mock and pathogen inoculated resistant (HD2329+Lr24) and susceptible (HD2329) near-isogenic wheat plants.Leaf rust ingression
PRJNA270216Triticum aestivum Transcriptome or Gene expressionIdentification and comparative analysis of differentially expressed miRNAs in leaves of two wheat (Triticum aestivum L.) genotypes during dehydration stressIllumina HiSeq 2000To identify those miRNAs involved in dehydration stress tolerance.Dehydration stress
PRJNA289147Triticum aestivum infected with Puccinia striiformis: small RNA and PARE (degradome) sequencingAnalysis of miRNAs in Two Wheat Cultivars Infected With Puccinia striiformis f. sp. triticiIon Torrent Proton,Illumina HiSeq 2500The fungus Puccinia striiformis causes stripe rust disease in cereal crops. This project contains raw sequence reads from small RNA sequencing and Parallel Analysis of RNA Ends (PARE, i.e. degradome-seq). Both infected and uninfected wheat are featured.Infected with Puccinia striiformis
PRJNA326902Deep sequencing of wheat sRNA transcriptome reveals distinct temporal expression pattern of miRNAs in response to heat, light and UVDeep sequencing of wheat sRNA transcriptome reveals distinct temporal expression pattern of miRNAs in response to heat, light and UVIllumina HiSeq 2000Understanding of plant adaptation to abiotic stresses has implications in plant breeding, especially in the context of climate change. MicroRNAs (miRNAs) and short interfering RNAs play a crucial role in gene regulation. Here, wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). A total of 72 small RNA libraries were sequenced on the Illumina platform generating ~524 million reads corresponding to ~129 million distinct tags from which 232 conserved miRNAs were identified. The expression levels of 1, 2 and 79 miRNAs were affected by ultraviolet radiations, continuous light and heat, respectively. Approximately 55% of the differentially expressed miRNAs were downregulated at 0 and 1 DAT including miR398, miR528 and miR156 that control mRNAs involved in activation of signal transduction pathways and flowering. Other putative targets included histone variants and methyltransferases. These results suggest a temporal miRNA-guided post-transcriptional regulation that enables wheat to respond to abiotic stresses, particularly heat. Designing novel wheat breeding strategies such as regulatory gene-based marker assisted selection depends on accurate identification of stress induced miRNAs. Overall design: Wheat plants were exposed to one of the following stresses: continuous light, heat or ultraviolet radiations over five consecutive days and, leaf tissues from three biological replicates were harvested at 0, 1, 2, 3, 7 and 10 days after treatment (DAT). A total of 72 small RNA libraries were sequenced on the Illumina platform.Response to heat, light and UV
PRJNA374855sRNAs from Triticum aestivum and Delphacodes kuscheli Raw sequence readsMal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper VectorIllumina HiSeq 1500Comparative analysis of sRNAs in MRCV (Mal de Río Cuarto virus) infected plants (wheat) and insects (D. kuscheli).Mal de Río Cuarto virus Infection
PRJNA392981Triticum aestivum Raw sequence readsGenome-Wide Identification and Characterization of Long Non-Coding RNA in Wheat Roots in Response to Ca2+ Channel BlockerIllumina HiSeq 2500Search different expression mRNA in wheat root induced by Ca2+-channel blocking.Ca2+-channel blocking
PRJNA420197sRNA sequencing of wheat introgression lines treated by saline or alkaline stressSmall RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheatIllumina HiSeq 2000To identify sRNA relative to saline/alkaline stress in wheat.Saline/Alkaline stress
PRJNA511811sRNA sequencing of wheat infected with BYDV-GAVBarley yellow dwarf virus-GAV-derived vsiRNAs are involved in the production of wheat leaf yellowing symptoms by targeting chlorophyll synthaseBGISEQ-500Obtaining total sRNA data from wheat leaves infected with BYDV-GAV.Infected with BYDV-GAV
PRJNA523507microRNA to patassium deprivation of wheatGlobal identification and characterization of miRNA family members responsive to potassium deprivation in wheat (Triticum aestivum L.)Illumina HiSeq 2500To provide insights into micorRNA to patassium deprivation in the seeding stage of wheat.Patassium deprivation
PRJNA553193Small RNA-seq from hexaploid bread wheat (Triticum aestivum) infected with wheat powdery mildewDomestication of High-Copy Transposons Underlays the Wheat Small RNA Response to an Obligate PathogenIllumina HiSeq 2500Sequencing data from wheat leaf material infected with three different powdery mildew (B.g. tritici ) isolates and from uninfected leaves. The goal of the project is to investigate the wheat small RNA response to wheat powdery mildew infection.Powdery mildew
PRJNA563099miRNA in wheat response to different N levelIdentification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levelsIllumina HiSeq 2000Study the key miRNA related to nitrogen stress during wheat develoment.Nitrogen stress
PRJNA698286Triticum aestivum Raw sequence readsComparative Small RNA Profiling and Functional Exploration on Wheat With High- and Low-Cadmium AccumulationHiSeq X TenTo find out the miRNAs related to the absorption of heavy metal cadmium in wheat.Heavy metal cadmium
PRJNA799639Object to identify drought memory-related miRNA in wheatIntegrate Small RNA and Degradome Sequencing to Reveal Drought Memory Response in Wheat (Triticum aestivum L.)Illumina HiSeq 2500This study identified drought memory response miRNAs and their target genes in wheat by small RNA.Trileaf stage wheat seedlings were divided into three groups: control group (CG), direct drought (DD) and drought memory (DM) groups.Drought memory
PRJNA837867Triticum aestivum Raw sequence readsIdentification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.)Illumina NovaSeq 6000Drought induced miRNAome of two wheat (Triticum aestivum L.) varieties.Drought
PRJNA877088Wheat spikelet total mRNA sequencingHeat-induced RING/U-BOX E3 ligase, TaUHS, is a negative regulator by facilitating TaLSD degradation during the grain filling period in wheatHiSeq X TenAnalysis total RNA expression under heat stress during grain-filling stage.Heat stress
PRJNA895954Whole transcriptome resequencing for temperature-mediated seed dormancy regulation in wheatIdentification and validation of coding and non-coding RNAs involved in high-temperature-mediated seed dormancy in common wheatIllumina HiSeq 4000Identification of mRNAs lncRNAs and miRNAs involved in temperature-mediated seed dormancy regulation in wheat.Temperature-mediated seed dormancy regulation
PRJNA916207System analysis of differentially expressed miRNAs in hexaploid wheat display tissue-specific regulatory role during Fe deficiency responseSystem analysis of differentially expressed miRNAs in hexaploid wheat display tissue-specific regulatory role during Fe deficiency responseIllumina NovaSeq 6000Iron (Fe) deficiency in soil could largely affect crop productivity but the molecular mechanisms underlying the regulation of Fe deficiency response are still limited. Specifically, microRNA (miRNA) mediated regulation of Fe deficiency genes and the network is poorly understood. In the current work, we aim to understand the molecular mechanisms of Fe deficiency response in wheat seedlings by generating an inventory of differentially expressed miRNAs. Stem loop quantitative RT PCR analysis suggested the expression of these miRNAs in a spatio-temporal fashion, with few showing time-specific expression responses. Furthermore, miRNA target prediction analysis suggested that few of these miRNA could specifically target genes such as multicopper oxidases, E3 ubiquitin ligases, GRAS family and WRKY transcription factors known to be involved in Fe homeostasis. Our work provides insights into miRNA-mediated regulatory pathways during Fe deficiency response. The first information generated here will help to identify the candidate genes for genetic improvement of Fe deficiency tolerance in hexaploid wheat.Iron (Fe) deficiency
PRJNA929049miRNA sequencing of three Turkish wheat speciesDiverse expression pattern of wheat transcription factors against abiotic stresses in wheat speciesIllumina HiSeq 2000This is a comparison study about miRNA libraries of three Turkish wheat cultivars in different ploidy levels (Triticum aestivum cv. Yuregir-89, Triticum turgidum cv. Kiziltan-91 and Triticum monococcum). In this project, it was aimed to reveal the miRNAs that play a role in drought stress tolerance by investigating the differences in miRNA expression profiles under drought stress among one drought tolerant (Kiziltan-91), another moderately drought tolerant (Yuregir-89) and ancestral wheat species. For this purpose, total RNA isolation was performed by collecting root and leaf samples from control and stressed plants and sRNA-Seq was performed using next generation sequencing (NGS) technology.Drought stress tolerance
PRJNA683746sRNA-seq and RNA-seq of wheat inoculating Fusarium graminearumIdentification of Wheat LACCASEs in Response to Fusarium graminearum as Potential Deoxynivalenol TrappersBGISEQ-500Elucidate the mechanism of wheat-Fusarium graminearum interaction.Fusarium graminearum
PRJNA791687Transcriptome of Triticum aestivum genotypes associated with resistance against the Wheat dwarf virusTranscriptome Dynamics in Triticum aestivum Genotypes Associated with Resistance against the Wheat Dwarf VirusNextSeq 500Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived Triticum aestivum transcriptome (RNA-seq) profiling methods and to evaluate genotypes associated with resistance against the Wheat dwarf virus. Methods: Triticum aestivum mRNA profiles of genotypes associated with resistance against the Wheat dwarf virus were generated by deep sequencing, in four replicates, using Illumina. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: Our study represents the first detailed analysis of Triticum aestivum transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA and miRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions. Overall design: Triticum aestivum mRNA profiles of genotypes associated with resistance against the Wheat dwarf virus were generated.Wheat dwarf virus
PRJNA960906Small RNA sequencing of wheat infected with stem rustDual host-pathogen small RNA sequencing during wheat stem rust infectionIon Torrent ProtonThis experiment isolated small RNA from Triticum aestivum seedlings infected with Puccinia graminis f.sp. tritici, in order to study gene regulation during a biotrophic interaction. Three infected replicates and three mock-inoculated replicates. Libraries were sequenced on the Ion Torrent platform.Wheat stem rust infection

Project ID Title Publication Instrument Description Notes
PRJDB11752sRNA transcriptome of wheat in response to stem rust and leaf rust interactionStem and leaf rust–induced miRNAome in bread wheat near-isogenic lines and their comparative analysisIllumina HiSeq 2500The R-gene mediated resistance has been employed as an efficient approach to control the rust diseases in wheat, one of the dreadful biotrophic fungal diseases affecting crop yield. The R-genes Sr36 and Lr45 have proven their ability in controlling highly virulent strains of stem rust and leaf rust diseases respectively. However, these R-gene mediated modulation over the regulatory non-coding RNAs was unclear. We demonstrated the alteration in non-coding RNA profiles during the resistant and susceptible interaction of wheat with stem rust and leaf rust using Sr36 and Lr45 carrying lines as resistant lines. Puccinia graminis pathotype 40A and Puccinia triticina pathotype 77-5 were used for the infection treatment whereas, water was used for the mock treatment. The non-coding RNA profiles obtained in this study will be a resource to the expanding rust disease management program.R-gene
PRJNA383463Asymmetrical changes in chromatin and small RNAs contribute to altered gene expression and endosperm development in resynthesized wheat allotetraploidsAsymmetrical changes of gene expression, small RNAs and chromatin in two resynthesized wheat allotetraploidsIllumina HiSeq 2000We performed genome-wide analyses of mRNA and small RNA transcriptomes in the endosperm of two synthetic wheats SSAA and AADD that differ in seed fertility and size. Transcriptomes between the endosperm and root of AADD were also analyzed to test a developmental role. Immunostaining experiments were performed to analyze the changes of histone H3K9me2 modifications in the chromosomes of two wheat allotetraploids and their progenitors.Intergenic spacer (IGS)
PRJEB24307Transcriptomic analysis of Fusarium graminearum disease response in near-isogenic hexaploid wheat lines differeing for the presence of a resistance QTL on chromosome 2DLComparative Transcriptome Profiles of Near-Isogenic Hexaploid Wheat Lines Differing for Effective Alleles at the 2DL FHB Resistance QTLIllumina Genome Analyzer IIxTo mine the molecular response associated with the wheat 2DL FHB resistance QTL and to identify candidate genes implicated in such esistance, a comprehensive transcriptomic analysis of the early response to F. graminearum infection in spikelet and rachis was performermed with the RNA-Seq and miRNA-Seq techniques. The analyses were conducted on two near isogenic lines differing for the presence of the 2DL QTL. The response to fungal infection in terms of mRNAs accumulation trend was similar in both NILs, even if at higher intensity in the susceptible NIL, and involved inhibition of primary metabolism and activation of secondary metabolism, amino acid metabolism, cell all reinforcement and remodelling, scavenging of ROS, hormone metabolism and signalling, detoxification and induction of several genes encoding transcription factors and proteins implicated in pathogens resistance response. To search for candidate genes with expression profiles associated with the 2DL QTL resistance led to the discovery of two different strategies associated with sugar signalling in the two NILs. Two genes associated with FHB suscaptibility were discovered. Altered expression was also observed for fungal non-coding RNAs which putative targets were represented by the WIR1A gene, involved in resistance response, and a gene encoding for a jacalin-related lectin protein, which partecipate in biotic and abiotic stress response. The data support the presence of a cross-talk between the plant and the fungus.F. graminearum infection
PRJEB28454Analysis of small RNA silencing in Zymoseptoria tritici – wheat interactionsAnalysis of small RNA silencing in Zymoseptoria tritici – wheat interactionsIllumina HiSeq 2000Deep sequencing of small RNA preparations from Zymoseptoria tritici isolate IPO323 cultured in vitro (in Czapek-Dox Broth, CDB) and those from the infected wheat cv. Bobwhite leaf tissues sampled at the four different time points corresponding to the four critical phases of Septoria tritici blotch disease development, namely 4 dpi (asymptomatic phase), 9 dpi (transition to necrotrophic stage), 13 dpi (necrotrophic phase), and 21 dpi (profuse asexual sporulation) was carried out on an Illumina HiSeq 2000 using 50 cycle single end reads. Deep sequencing of small RNA preparations from mock-inoculated wheat cv. Bobwhite leaves sampled at 4 dpi, 9 dpi, 13 dpi, and 21 dpi and from healthy, untreated 17 days old wheat cv. Bobwhite leaves was also carried out using the same sequencing technology.4 dpi, 9 dpi, 13 dpi, and 21 dpi
PRJNA242577Triticum aestivumIdentification of Novel miRNAs and miRNA Expression Profiling in Wheat Hybrid NecrosisIllumina HiSeq 2000Identification of Novel miRNAs and miRNA Expression Profiling in Wheat Hybrid Necrosis.Wheat hybrid necrosis
PRJNA347868Triticum aestivum Raw sequence readsGmDREB1 overexpression affects the expression of microRNAs in GM wheat seedsIllumina HiSeq 2500In this study, we performed deep sequencing of sRNAs from a transgenic wheat line overexpressing GmDREB1 and non-transgenic wheat varieties.GmDREB1 overexpression
PRJNA353049Transcriptome and Epigenome analysis in Polyploidy Wheat and its progenitorsNew insights on the evolution of nucleolar dominance in newly resynthesized hexaploid wheat Triticum zhukovskyiIllumina HiSeq 2000We analyzed sequence features of intergenic spacer (IGS) in the progenitors of common wheat Triticum aestivum (AABBDD), which revealed nucleolar organizing region (NOR) dominance hierarchy (BB > DD > AA). The hierarchy is based on the length, transcript start site, number and sequence variation of Rep1 (promoter-like repeat) unit. Transcriptome and epigenome in the synthetic tetraploid wheat (AADD / DDAA) and their progenitors T. urartu (AA) and Ae. tauschii (DD) were further investigated to explore the mechanism for NOR silencing. We found that long non-coding RNAs and 24-nt small interfering RNAs were highly abundant in the IGS regions of the DD, and scarcity in AA. After polyploidy formation, NORs of AA subgenome were silenced and associated with increased levels of 24-nt siRNAs derived from active NOR of the DD subgenome. These DD-subgenome 24-nt siRNAs may trans-act on AA-genome IGS to induce DNA methylation via RNA directed DNA methylation (RdDM). Furthermore, BS-Seq and MeDIP-Seq assay show siRNA-targeted regions in AA-subgenome IGS were hypermethylated. Additionally, the increased H3K27me3 and decreased H4K12ac were found in AA-subgenome IGS after polyploidization. This similar phenomenon of BB 24-nt siRNAs trans-act on DD was detected in hexaploid wheat.Histone H3K9me2
PRJNA353130Global studies of miR021b function in wheatWheat miR9678 Affects Seed Germination by Generating Phased siRNAs and Modulating Abscisic Acid/Gibberellin SignalingIllumina HiSeq 2500Seed germination is not only a critical developmental step in the wheat life cycle, but is also important for agricultural production including yield and quality. However, in wheat, the knowledge of the mechanism of regulating seed germination is still limited. In this study, we found 22nt microRNA (miR) miR021b, specifically expressed in scutellum of developing and germinating wheat seed, generated phased ta-siRNAs by cleaving a long non coding RNA LNCR. Overexpression of miR021b in wheat showed a retarded germination and improved resistance to pre-harvest sprouting (PHS), while its silencing enhanced germination rate through transiently expressing in immature embryos. To figure out the mechanism of miR021b regulating seed germination, we found miR021b affected the expression of genes involved in bioactive gibberellin (GA) synthesis and its overexpression reduced the bioactive GA content and inhibited amylase genes expression. In addition, it was observed that TaVp1, TaABF and TaABI3, responded the abscisic acid (ABA) signaling, can bind the promoter of miR021b precursor and regulated its expression, suggesting that miR021b might function in GA-ABA balance during germination. This study identified a signaling pathway that miR021b controlled GA-dependent seed germination in wheat through generating phased ta-siRNAs by cleaved a long non coding RNA LNCR.miR021b function
PRJNA380529Triticum aestivum Transcriptome or Gene expressionUncovering key small RNAs associated with gametocidal action in wheatIllumina Genome AnalyzerTwo small RNA libraries were constructed from T. Aestivum cv. Chinese Spring (CS) and Chinese Spring-gametocidal 3C chromosome monosomic addition line (CS-3C) and sequenced using Illumina high throughput sequencing technology to investigate whether small RNAs play a role in the gametocidal phenomenon.Gametocidal action
PRJNA514418Mutation of a histidine-rich calcium-binding protein gene in wheat confers resistance to Fusarium head blightMutation of a histidine-rich calcium-binding-protein gene in wheat confers resistance to Fusarium head blightIllumina HiSeq 2500We isolated the major-effect wheat QTL, Qfhs.njau-3B, that confers head blight resistance and showed that it is the same as the previously designated Fhb1. Fhb1 results from a rare deletion involving the 3’ exon of the histidine-rich calcium-binding protein gene on chromosome 3BS. Both wheat and Arabidopsis transformed with the Fhb1 sequence showed enhanced resistance to F. graminearum infection. The translation products of this gene’s homologs among plants are well conserved and might be essential to plant growth and development. Fhb1 could be useful not only for curbing FHB diseases in grain crops but also for improving other plants vulnerable to Fusarium spp.Fhb1
PRJNA554945Triticum aestivum Raw sequence readsEnhanced Senescence Process is the Major Factor Stopping Spike Differentiation of Wheat Mutant ptsd1Illumina HiSeq 2500Transcriptomes and miRNomes of the wide type (WT) and mutant ptsd1 at the early spike differentiation stage.Wheat Mutant ptsd1
PRJNA578261Wheat anther miRNA sequencingTaEXPB5 functions as a gene related to pollen development in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasmHiSeq X TenTo reveal the mechanism of thermosensitive male sterility in wheat.TaEXPB5 functions
PRJNA915324Wheat RNA sequencingNew insights on the evolution of nucleolar dominance in newly resynthesized hexaploid wheat Triticum ZhukovskyiIllumina HiSeq 2500RNA-seq was carried out using material from seedling betweenl AG x A hybridization F1 and AG x D hybridization F1 .Leaf 30D

Sample Information

Run Library Selection Library Name Age Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR24282674size fractionationI-0214 dayswhole leafMcNair 701Infected_02USA: Pullmanrep2
SRR24282675size fractionationI-0114 dayswhole leafMcNair 701Infected_01USA: Pullmanrep1
SRR24282676size fractionationU-0314 dayswhole leafMcNair 701Uninfected_03USA: Pullmanrep3
SRR24282677size fractionationU-0214 dayswhole leafMcNair 701Uninfected_02USA: Pullmanrep2
SRR24282678size fractionationU-0114 dayswhole leafMcNair 701Uninfected_01USA: Pullmanrep1

Run Library Selection Library Name Ecotype Stage Tissue Cultivar Sample Name Geographic Location Name
SRR23262625cDNATaSRootTurkeyTilleringRootYuregir-89TaSRootTurkey: Kastamonu
SRR23262626cDNATaCRootTurkeyTilleringRootYuregir-89TaCRootTurkey: Kastamonu
SRR23262629cDNATaSLeafTurkeyTilleringLeafYuregir-89TaSLeafTurkey: Kastamonu
SRR23262630cDNATaCLeafTurkeyTilleringLeafYuregir-89TaCLeafTurkey: Kastamonu
SRR23262621cDNATtSRootTurkeyTilleringRootKiziltan-91TtSRootTurkey: Kastamonu
SRR23262622cDNATtCRootTurkeyTilleringRootKiziltan-91TtCRootTurkey: Kastamonu
SRR23262623cDNATtSLeafTurkeyTilleringLeafKiziltan-91TtSLeafTurkey: Kastamonu
SRR23262624cDNATtCLeafTurkeyTilleringLeafKiziltan-91TtCLeafTurkey: Kastamonu
SRR23262619cDNATmSLeafTurkeyTilleringLeafSiyezTmSLeafTurkey: Kastamonu
SRR23262620cDNATmCLeafTurkeyTilleringLeafSiyezTmCLeafTurkey: Kastamonu
SRR23262627cDNATmSRootTurkeyTilleringRootSiyezTmSRootTurkey: Kastamonu
SRR23262628cDNATmCRootTurkeyTilleringRootSiyezTmCRootTurkey: Kastamonu

Run Library Selection Library Name Ecotype Age Tissue Cultivar Sample Name Geographic Location Name Treatment
SRR22989766PCR(-Fe) SHOOTNABI MOHALI2 weeksSHOOTC306(-Fe) SHOOTIndiaFe Deficient
SRR22989767PCR(+Fe) SHOOTNABI MOHALI2 weeksSHOOTC306(+Fe) SHOOTIndiaFe sufficient
SRR22989768PCR(-Fe) ROOTNABI MOHALI2 weeksROOTC306(-Fe) ROOTIndiaFe Deficient
SRR22989769PCR(+Fe) ROOTNABI MOHALI2 weeksROOTC306(+Fe) ROOTIndiaFe sufficient

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Treatment
SRR22103146RT-PCRWheat_L263_S1035DPAdeveloping seedsWaitoubaiWheat_L263_10Normal temperature
SRR22103147RT-PCRWheat_L263_S0928DPAdeveloping seedsWaitoubaiWheat_L263_09High temperature
SRR22103148RT-PCRWheat_L263_S0828DPAdeveloping seedsWaitoubaiWheat_L263_08High temperature
SRR22103149RT-PCRWheat_L263_S0728DPAdeveloping seedsWaitoubaiWheat_L263_07High temperature
SRR22103150RT-PCRWheat_L263_S0628DPAdeveloping seedsWaitoubaiWheat_L263_06Normal temperature
SRR22103151RT-PCRWheat_L263_S0528DPAdeveloping seedsWaitoubaiWheat_L263_05Normal temperature
SRR22103152RT-PCRWheat_L263_S0428DPAdeveloping seedsWaitoubaiWheat_L263_04Normal temperature
SRR22103163RT-PCRWheat_L263_S0321DPAdeveloping seedsWaitoubaiWheat_L263_03Normal temperature
SRR22103169RT-PCRWheat_L263_S1535DPAdeveloping seedsWaitoubaiWheat_L263_15High temperature
SRR22103170RT-PCRWheat_L263_S1435DPAdeveloping seedsWaitoubaiWheat_L263_14High temperature
SRR22103171RT-PCRWheat_L263_S1335DPAdeveloping seedsWaitoubaiWheat_L263_13High temperature
SRR22103172RT-PCRWheat_L263_S1235DPAdeveloping seedsWaitoubaiWheat_L263_12Normal temperature
SRR22103173RT-PCRWheat_L263_S1135DPAdeveloping seedsWaitoubaiWheat_L263_11Normal temperature
SRR22103174RT-PCRWheat_L263_S0221DPAdeveloping seedsWaitoubaiWheat_L263_02Normal temperature
SRR22103175RT-PCRWheat_L263_S0121DPAdeveloping seedsWaitoubaiWheat_L263_01Normal temperature

Run Library Selection Library Name Ecotype Age Tissue Cultivar Sample Name Geographic Location Name
SRR21439292Oligo-dTDAC10_2korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439293Oligo-dTDAC10_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439294Oligo-dTDAT10_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439295Oligo-dTDAC6_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul
SRR21439296Oligo-dTDAT6_1korea3 monthsSpikeletKeumgangRNA under heat stressSouth Korea: Seoul

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR21050554othersmallTFL-1-1heading dateovaryDong xuan 3smallTFL-1-1Chinareplicate=biological replicate 10
SRR21050555othersmallCK-3-3heading dateovaryDong xuan 3smallCK-3-3Chinareplicate=biological replicate 9
SRR21050556othersmallCK-3-2heading dateovaryDong xuan 3smallCK-3-2Chinareplicate=biological replicate 8
SRR21050557othersmallCK-3-1heading dateovaryDong xuan 3smallCK-3-1Chinareplicate=biological replicate 7
SRR21050558othersmallCK-2-3heading dateovaryDong xuan 3smallCK-2-3Chinareplicate=biological replicate 6
SRR21050559othersmallCK-2-2heading dateovaryDong xuan 3smallCK-2-2Chinareplicate=biological replicate 5
SRR21050560othersmallCK-2-1heading dateovaryDong xuan 3smallCK-2-1Chinareplicate=biological replicate 4
SRR21050561othersmallCK-1-3heading dateovaryDong xuan 3smallCK-1-3Chinareplicate=biological replicate 3
SRR21050562othersmallTFL-3-3heading dateovaryDong xuan 3smallTFL-3-3Chinareplicate=biological replicate 18
SRR21050563othersmallTFL-3-2heading dateovaryDong xuan 3smallTFL-3-2Chinareplicate=biological replicate 17
SRR21050564othersmallTFL-3-1heading dateovaryDong xuan 3smallTFL-3-1Chinareplicate=biological replicate 16
SRR21050565othersmallTFL-2-3heading dateovaryDong xuan 3smallTFL-2-3Chinareplicate=biological replicate 15
SRR21050566othersmallTFL-2-2heading dateovaryDong xuan 3smallTFL-2-2Chinareplicate=biological replicate 14
SRR21050567othersmallTFL-2-1heading dateovaryDong xuan 3smallTFL-2-1Chinareplicate=biological replicate 13
SRR21050568othersmallTFL-1-3heading dateovaryDong xuan 3smallTFL-1-3Chinareplicate=biological replicate 12
SRR21050569othersmallTFL-1-2heading dateovaryDong xuan 3smallTFL-1-2Chinareplicate=biological replicate 11
SRR21050570othersmallCK-1-2heading dateovaryDong xuan 3smallCK-1-2Chinareplicate=biological replicate 2
SRR21050571othersmallCK-1-1heading dateovaryDong xuan 3smallCK-1-1Chinareplicate=biological replicate 1

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Treatment
DRR299372cDNALeaf rust Susceptible- Mock inoculationTwo-leaf stageleafHD2329LSMpathogen Infection
DRR299371cDNAStem rust resistant- Infection treatmentTwo-leaf stageleafHD2329+Sr36SRIMock
DRR299374cDNALeaf rust Resistant- Mock inoculationTwo-leaf stageleafHD2329 +Lr45SAMD00325718Mock
DRR299373cDNALeaf rust Susceptible- Infection treatedTwo-leaf stageleafHD2329SAMD00325717pathogen Infection
DRR299375cDNALeaf rust Resistant-Infection treatedTwo-leaf stageleafHD2329+Lr45SAMD00325719pathogen Infection
DRR299370cDNAStem rust Resistant- Mock inoculationTwo-leaf stageleafHD2329 +Sr36SAMD00325714Mock
DRR299369cDNAStem rust susceptible- Infection treatmentTwo-leaf stageleafHD2329SAMD00325713pathogen Infection
DRR299368cDNAStem rust susceptible- Mock inoculationTwo-leaf stageleafHD2329SAMD00325712Mock

Run Library Name Age Tissue Cultivar Sample Name Geographic Location Name Treatment Replicate
SRR2094581Infected Louise 342 daysflag leafLouiseInfected LouiseUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094580Infected Louise 242 daysflag leafLouiseInfected LouiseUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094579Infected Louise 142 daysflag leafLouiseInfected LouiseUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094591Uninfected Louise 342 daysflag leafLouiseUninfected LouiseUSA: Washingtonmock inoculated with talcum powder-
SRR2094590Uninfected Louise 242 daysflag leafLouiseUninfected LouiseUSA: Washingtonmock inoculated with talcum powder-
SRR2094588Uninfected Louise 142 daysflag leafLouiseUninfected LouiseUSA: Washingtonmock inoculated with talcum powder-
SRR2094566Infected Penawawa 342 daysflag leafPenawawaInfected PenawawaUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094565Infected Penawawa 242 daysflag leafPenawawaInfected PenawawaUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094492Infected Penawawa 142 daysflag leafPenawawaInfected PenawawaUSA: Washingtoninoculated with urediospores plus talcum powder-
SRR2094586Uninfected Penawawa 342 daysflag leafPenawawaUninfected PenawawaUSA: Washingtonmock inoculated with talcum powder-
SRR2094585Uninfected Penawawa 242 daysflag leafPenawawaUninfected PenawawaUSA: Washingtonmock inoculated with talcum powder-
SRR2094584Uninfected Penawawa 142 daysflag leafPenawawaUninfected PenawawaUSA: Washingtonmock inoculated with talcum powder-
SRR19441421I4_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI4_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 4
SRR19441422I3_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI3_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 3
SRR19441423I2_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI2_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 2
SRR19441424I1_AvS_Pst78_sRNA22 dayswhole shootAvocet SusceptibleI1_AvS_Pst78_18USA: Washington2 week old plants inoculated with Pst78 spores; dew chamber 24h; incubated 7 days 15C day/15C nightInfected 1
SRR19441425U4_AvS_sRNA22 dayswhole shootAvocet SusceptibleU4_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 4
SRR19441426U3_AvS_sRNA22 dayswhole shootAvocet SusceptibleU3_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 3
SRR19441427U2_AvS_sRNA22 dayswhole shootAvocet SusceptibleU2_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 2
SRR19441428U1_AvS_sRNA22 dayswhole shootAvocet SusceptibleU1_AvS_18USA: Washington2 week old plants mock-inoculated with talc; dew chamber 24h; incubated 7 days 15C day/15C nightUninfected 1

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Treatment Replicate
SRR19238050size fractionationDT-T-1seedling stagerootZM13DT-T-1China:jinzhongdroughtbiological replicate 1
SRR19238051size fractionationDT-CK-3seedling stagerootZM13DT-CK-3China:jinzhongnormalbiological replicate 3
SRR19238052size fractionationDT-CK-2seedling stagerootZM13DT-CK-2China:jinzhongnormalbiological replicate 2
SRR19238053size fractionationDT-CK-1seedling stagerootZM13DT-CK-1China:jinzhongnormalbiological replicate 1
SRR19238054size fractionationDS-T-3seedling stagerootJM38DS-T-3China:jinzhongdroughtbiological replicate 3
SRR19238055size fractionationDS-T-2seedling stagerootJM38DS-T-2China:jinzhongdroughtbiological replicate 2
SRR19238056size fractionationDS-T-1seedling stagerootJM38DS-T-1China:jinzhongdroughtbiological replicate 1
SRR19238057size fractionationDS-CK-3seedling stagerootJM38DS-CK-3China:jinzhongnormalbiological replicate 3
SRR19238058size fractionationDT-T-3seedling stagerootZM13DT-T-3China:jinzhongdroughtbiological replicate 3
SRR19238059size fractionationDT-T-2seedling stagerootZM13DT-T-2China:jinzhongdroughtbiological replicate 2
SRR19238060size fractionationDS-CK-2seedling stagerootJM38DS-CK-2China:jinzhongnormalbiological replicate 2
SRR19238061size fractionationDS-CK-1seedling stagerootJM38DS-CK-1China:jinzhongnormalbiological replicate 1

Run Library Selection Library Name Stage Tissue Sample Name Geographic Location Name
SRR17712120PCRCG_6h_1Trileaf stageWhole seedlingsCG_6h_1China:shannxi
SRR17712121PCRCG_1h_3Trileaf stageWhole seedlingsCG_1h_3China:shannxi
SRR17712122PCRCG_1h_2Trileaf stageWhole seedlingsCG_1h_2China:shannxi
SRR17712123PCRCG_1h_1Trileaf stageWhole seedlingsCG_1h_1China:shannxi
SRR17712124PCRCG_12h_3Trileaf stageWhole seedlingsCG_12h_3China:shannxi
SRR17712125PCRCG_12h_2Trileaf stageWhole seedlingsCG_12h_2China:shannxi
SRR17712126PCRDD_6h_3Trileaf stageWhole seedlingsDD_6h_3China:shannxi
SRR17712127PCRDD_6h_2Trileaf stageWhole seedlingsDD_6h_2China:shannxi
SRR17712128PCRDD_6h_1Trileaf stageWhole seedlingsDD_6h_1China:shannxi
SRR17712129PCRDD_1h_3Trileaf stageWhole seedlingsDD_1h_3China:shannxi
SRR17712130PCRDD_1h_2Trileaf stageWhole seedlingsDD_1h_2China:shannxi
SRR17712131PCRDD_1h_1Trileaf stageWhole seedlingsDD_1h_1China:shannxi
SRR17712132PCRCG_12h_1Trileaf stageWhole seedlingsCG_12h_1China:shannxi
SRR17712133PCRDD_12h_3Trileaf stageWhole seedlingsDD_12h_3China:shannxi
SRR17712134PCRDD_12h_2Trileaf stageWhole seedlingsDD_12h_2China:shannxi
SRR17712135PCRDD_12h_1Trileaf stageWhole seedlingsDD_12h_1China:shannxi
SRR17712136PCRDD_0h_3Trileaf stageWhole seedlingsDD_0h_3China:shannxi
SRR17712137PCRDD_0h_2Trileaf stageWhole seedlingsDD_0h_2China:shannxi
SRR17712138PCRDD_0h_1Trileaf stageWhole seedlingsDD_0h_1China:shannxi
SRR17712139PCRDM_6h_3Trileaf stageWhole seedlingsDM_6h_3China:shannxi
SRR17712140PCRDM_6h_2Trileaf stageWhole seedlingsDM_6h_2China:shannxi
SRR17712141PCRDM_6h_1Trileaf stageWhole seedlingsDM_6h_1China:shannxi
SRR17712142PCRDM_1h_3Trileaf stageWhole seedlingsDM_1h_3China:shannxi
SRR17712143PCRCG_0h_3Trileaf stageWhole seedlingsCG_0h_3China:shannxi
SRR17712144PCRDM_1h_2Trileaf stageWhole seedlingsDM_1h_2China:shannxi
SRR17712145PCRDM_1h_1Trileaf stageWhole seedlingsDM_1h_1China:shannxi
SRR17712146PCRDM_12h_3Trileaf stageWhole seedlingsDM_12h_3China:shannxi
SRR17712147PCRDM_12h_2Trileaf stageWhole seedlingsDM_12h_2China:shannxi
SRR17712148PCRDM_12h_1Trileaf stageWhole seedlingsDM_12h_1China:shannxi
SRR17712149PCRDM_0h_3Trileaf stageWhole seedlingsDM_0h_3China:shannxi
SRR17712150PCRDM_0h_2Trileaf stageWhole seedlingsDM_0h_2China:shannxi
SRR17712151PCRDM_0h_1Trileaf stageWhole seedlingsDM_0h_1China:shannxi
SRR17712152PCRCG_6h_3Trileaf stageWhole seedlingsCG_6h_3China:shannxi
SRR17712153PCRCG_6h_2Trileaf stageWhole seedlingsCG_6h_2China:shannxi
SRR17712154PCRCG_0h_2Trileaf stageWhole seedlingsCG_0h_2China:shannxi
SRR17712155PCRCG_0h_1Trileaf stageWhole seedlingsCG_0h_1China:shannxi

Run Library Selection Library Name Tissue Cultivar Sample Name Treatment Replicate
SRR17316475size fractionationGSM5750485leavesAkteurGSM5750485Non inoculatedrep4
SRR17316476size fractionationGSM5750484leavesAkteurGSM5750484Non inoculatedrep3
SRR17316477size fractionationGSM5750483leavesAkteurGSM5750483Non inoculatedrep2
SRR17316478size fractionationGSM5750482leavesAkteurGSM5750482Non inoculatedrep1
SRR17316479size fractionationGSM5750481leavesAkteurGSM5750481Non inoculatedrep4
SRR17316480size fractionationGSM5750480leavesAkteurGSM5750480Non inoculatedrep3
SRR17316481size fractionationGSM5750479leavesAkteurGSM5750479Non inoculatedrep2
SRR17316482size fractionationGSM5750478leavesAkteurGSM5750478Non inoculatedrep1
SRR17316483size fractionationGSM5750477leavesAkteurGSM5750477WDV infectedrep4
SRR17316484size fractionationGSM5750476leavesAkteurGSM5750476WDV infectedrep3
SRR17316485size fractionationGSM5750475leavesAkteurGSM5750475WDV infectedrep2
SRR17316486size fractionationGSM5750474leavesAkteurGSM5750474WDV infectedrep1
SRR17316487size fractionationGSM5750473leavesAkteurGSM5750473WDV infectedrep4
SRR17316488size fractionationGSM5750472leavesAkteurGSM5750472WDV infectedrep3
SRR17316489size fractionationGSM5750471leavesAkteurGSM5750471WDV infectedrep2
SRR17316490size fractionationGSM5750470leavesAkteurGSM5750470WDV infectedrep1
SRR17316491size fractionationGSM5750469leavesAkteurGSM5750469WDV infectedrep4
SRR17316492size fractionationGSM5750468leavesAkteurGSM5750468WDV infectedrep3
SRR17316493size fractionationGSM5750467leavesAkteurGSM5750467WDV infectedrep2
SRR17316494size fractionationGSM5750466leavesAkteurGSM5750466WDV infectedrep1
SRR17316495size fractionationGSM5750465leavesAkteurGSM5750465Non inoculatedrep4
SRR17316496size fractionationGSM5750464leavesAkteurGSM5750464Non inoculatedrep3
SRR17316497size fractionationGSM5750463leavesAkteurGSM5750463Non inoculatedrep2
SRR17316498size fractionationGSM5750462leavesAkteurGSM5750462Non inoculatedrep1
SRR17316499size fractionationGSM5750533leavesAkteurGSM5750533Non inoculatedrep4
SRR17316500size fractionationGSM5750532leavesSvitavaGSM5750532Non inoculatedrep3
SRR17316501size fractionationGSM5750531leavesSvitavaGSM5750531Non inoculatedrep2
SRR17316502size fractionationGSM5750530leavesSvitavaGSM5750530Non inoculatedrep1
SRR17316503size fractionationGSM5750529leavesSvitavaGSM5750529Non inoculatedrep4
SRR17316504size fractionationGSM5750528leavesSvitavaGSM5750528Non inoculatedrep3
SRR17316505size fractionationGSM5750527leavesSvitavaGSM5750527Non inoculatedrep2
SRR17316506size fractionationGSM5750526leavesSvitavaGSM5750526Non inoculatedrep1
SRR17316507size fractionationGSM5750525leavesSvitavaGSM5750525Non inoculatedrep4
SRR17316508size fractionationGSM5750524leavesSvitavaGSM5750524Non inoculatedrep3
SRR17316509size fractionationGSM5750523leavesSvitavaGSM5750523Non inoculatedrep2
SRR17316510size fractionationGSM5750522leavesSvitavaGSM5750522Non inoculatedrep1
SRR17316511size fractionationGSM5750521leavesSvitavaGSM5750521WDV infectedrep4
SRR17316512size fractionationGSM5750520leavesSvitavaGSM5750520WDV infectedrep3
SRR17316513size fractionationGSM5750519leavesSvitavaGSM5750519WDV infectedrep2
SRR17316514size fractionationGSM5750518leavesSvitavaGSM5750518WDV infectedrep1
SRR17316515size fractionationGSM5750517leavesSvitavaGSM5750517WDV infectedrep4
SRR17316516size fractionationGSM5750516leavesSvitavaGSM5750516WDV infectedrep3
SRR17316517size fractionationGSM5750515leavesSvitavaGSM5750515WDV infectedrep2
SRR17316518size fractionationGSM5750514leavesSvitavaGSM5750514WDV infectedrep1
SRR17316519size fractionationGSM5750513leavesSvitavaGSM5750513WDV infectedrep4
SRR17316520size fractionationGSM5750512leavesSvitavaGSM5750512WDV infectedrep3
SRR17316521size fractionationGSM5750511leavesSvitavaGSM5750511WDV infectedrep2
SRR17316522size fractionationGSM5750510leavesSvitavaGSM5750510WDV infectedrep1
SRR17316571size fractionationGSM5750509leavesFengyouGSM5750509Non inoculatedrep4
SRR17316572size fractionationGSM5750508leavesFengyouGSM5750508Non inoculatedrep3
SRR17316573size fractionationGSM5750507leavesFengyouGSM5750507Non inoculatedrep2
SRR17316574size fractionationGSM5750506leavesFengyouGSM5750506Non inoculatedrep1
SRR17316575size fractionationGSM5750505leavesFengyouGSM5750505WDV infectedrep4
SRR17316576size fractionationGSM5750504leavesFengyouGSM5750504WDV infectedrep3
SRR17316577size fractionationGSM5750503leavesFengyouGSM5750503WDV infectedrep2
SRR17316578size fractionationGSM5750502leavesFengyouGSM5750502WDV infectedrep1
SRR17316579size fractionationGSM5750501leavesFengyouGSM5750501WDV infectedrep4
SRR17316580size fractionationGSM5750500leavesFengyouGSM5750500WDV infectedrep3
SRR17316581size fractionationGSM5750499leavesFengyouGSM5750499WDV infectedrep2
SRR17316582size fractionationGSM5750498leavesFengyouGSM5750498WDV infectedrep1
SRR17316583size fractionationGSM5750497leavesFengyouGSM5750497WDV infectedrep4
SRR17316584size fractionationGSM5750496leavesFengyouGSM5750496WDV infectedrep3
SRR17316585size fractionationGSM5750495leavesFengyouGSM5750495WDV infectedrep2
SRR17316586size fractionationGSM5750494leavesFengyouGSM5750494WDV infectedrep1
SRR17316587size fractionationGSM5750493leavesFengyouGSM5750493Non inoculatedrep4
SRR17316588size fractionationGSM5750492leavesFengyouGSM5750492Non inoculatedrep3
SRR17316589size fractionationGSM5750491leavesFengyouGSM5750491Non inoculatedrep2
SRR17316590size fractionationGSM5750490leavesFengyouGSM5750490Non inoculatedrep1
SRR17316591size fractionationGSM5750489leavesFengyouGSM5750489Non inoculatedrep4
SRR17316592size fractionationGSM5750488leavesFengyouGSM5750488Non inoculatedrep3
SRR17316593size fractionationGSM5750487leavesFengyouGSM5750487Non inoculatedrep2
SRR17316594size fractionationGSM5750486leavesFengyouGSM5750486Non inoculatedrep1

Run Library Selection Age Tissue Cultivar Sample Name Geographic Location Name
SRR13619124PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619125PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619126PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619127PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619128PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619129PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619130PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619131PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619132PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619133PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619134PCR10 daysroots and leaveswheatY174China:Anhui
SRR13619135PCR10 daysroots and leaveswheatY174China:Anhui

Run Library Selection Library Name Ecotype Stage Tissue Sample Name Geographic Location Name
SRR13222412PCRSMI-1Sumai3flowering phasespikelet and rachisSMChina
SRR13222413PCRSMM-3Sumai3flowering phasespikelet and rachisSMChina
SRR13222414PCRSMM-2Sumai3flowering phasespikelet and rachisSMChina
SRR13222415PCRSMM-1Sumai3flowering phasespikelet and rachisSMChina
SRR13222416PCRCSI-3Chinese springflowering phasespikelet and rachisCSChina
SRR13222417PCRCSI-2Chinese springflowering phasespikelet and rachisCSChina
SRR13222418PCRCSI-1Chinese springflowering phasespikelet and rachisCSChina
SRR13222419PCRCSM-3Chinese springflowering phasespikelet and rachisCSChina
SRR13222420PCRSMI-3Sumai3flowering phasespikelet and rachisSMChina
SRR13222421PCRSMI-2Sumai3flowering phasespikelet and rachisSMChina
SRR13222422PCRCSM-2Chinese springflowering phasespikelet and rachisCSChina
SRR13222423PCRCSM-1Chinese springflowering phasespikelet and rachisCSChina

Run Library Selection Stage Tissue Sample Name Treatment
SRR12419568size fractionationjointing stagecrown tissue colleected at jointing stageGSM4717211Non inoculated
SRR12419567size fractionationspring greenup stagecrown tissue colleected at spring greenup stageGSM4717210Non inoculated
SRR12419566size fractionationwinter dormancy stagecrown tissue colleected at winter dormancy stageGSM4717209Non inoculated
SRR12419565size fractionationthree leaf stagecrown tissue collected at the three leaf stageGSM4717208Non inoculated

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Treatment Replicate
SRR12102896size fractionationsRNA-seq_spikelet_rep3floweringSpikeletChinese spriingSpikelet_smallRNA_3China:Wuhansmall RNA-seq of wheatReplicate 3
SRR12102897size fractionationsRNA-seq_spikelet_rep2floweringSpikeletChinese spriingSpikelet_smallRNA_2China:Wuhansmall RNA-seq of wheatReplicate 2
SRR12102898size fractionationsRNA-seq_spikelet_rep1floweringSpikeletChinese spriingSpikelet_smallRNA_1China:Wuhansmall RNA-seq of wheatReplicate 1

Run Library Selection Library Name Age Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR10317096PCRSF1-1early uninucleateantherwheat TCMSSF1-1China:shannxibiological replicate1
SRR10317097PCRSS3-3binucleateantherwheat TCMSSS3-3China:shannxibiological replicate3
SRR10317098PCRSS3-2binucleateantherwheat TCMSSS3-2China:shannxibiological replicate2
SRR10317099PCRSS3-1binucleateantherwheat TCMSSS3-1China:shannxibiological replicate1
SRR10317100PCRSS2-3late uninucleateantherwheat TCMSSS2-3China:shannxibiological replicate3
SRR10317101PCRSS2-2late uninucleateantherwheat TCMSSS2-2China:shannxibiological replicate2
SRR10317102PCRSS2-1late uninucleateantherwheat TCMSSS2-1China:shannxibiological replicate1
SRR10317103PCRSS1-3early uninucleateantherwheat TCMSSS1-3China:shannxibiological replicate3
SRR10317104PCRSF3-3binucleateantherwheat TCMSSF3-3China:shannxibiological replicate3
SRR10317105PCRSF3-2binucleateantherwheat TCMSSF3-2China:shannxibiological replicate2
SRR10317106PCRSF3-1binucleateantherwheat TCMSSF3-1China:shannxibiological replicate1
SRR10317107PCRSF2-3late uninucleateantherwheat TCMSSF2-3China:shannxibiological replicate3
SRR10317108PCRSF2-2late uninucleateantherwheat TCMSSF2-2China:shannxibiological replicate2
SRR10317109PCRSF2-1late uninucleateantherwheat TCMSSF2-1China:shannxibiological replicate1
SRR10317110PCRSF1-3early uninucleateantherwheat TCMSSF1-3China:shannxibiological replicate3
SRR10317111PCRSF1-2early uninucleateantherwheat TCMSSF1-2China:shannxibiological replicate2
SRR10317112PCRSS1-2early uninucleateantherwheat TCMSSS1-2China:shannxibiological replicate2
SRR10317113PCRSS1-1early uninucleateantherwheat TCMSSS1-1China:shannxibiological replicate1

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Treatment
SRR10051481cDNAN0_17D_117_days_after_anthesisseedZhengmai119miRNA_LN17D1China:Zhengzhoudifferent nitrogen level
SRR10051482cDNAZ_17D_317_days_after_anthesisseedZhengmai119miRNA_HN17D3China:Zhengzhoudifferent nitrogen level
SRR10051483cDNAZ_17D_217_days_after_anthesisseedZhengmai119miRNA_HN17D2China:Zhengzhoudifferent nitrogen level
SRR10051484cDNAZ_17D_117_days_after_anthesisseedZhengmai119miRNA_HN17D1China:Zhengzhou-
SRR10051485cDNAN0_6D_36_days_after_anthesisseedZhengmai119miRNA_LN6D3China:Zhengzhou-
SRR10051486cDNAN0_6D_26_days_after_anthesisseedZhengmai119miRNA_LN6D2China:Zhengzhoudifferent nitrogen level
SRR10051487cDNAN0_6D_16_days_after_anthesisseedZhengmai119miRNA_LN6D1China:Zhengzhoudifferent nitrogen level
SRR10051488cDNAZ_6D_36_days_after_anthesisseedZhengmai119miRNA_HN6D3China:Zhengzhou-
SRR10051489cDNAN0_27D_327_days_after_anthesisseedZhengmai119miRNA_LN27D3China:Zhengzhoudifferent nitrogen level
SRR10051490cDNAN0_27D_227_days_after_anthesisseedZhengmai119miRNA_LN27D2China:Zhengzhoudifferent nitrogen level
SRR10051491cDNAN0_27D_127_days_after_anthesisseedZhengmai119miRNA_LN27D1China:Zhengzhoudifferent nitrogen level
SRR10051492cDNAZ_27D_327_days_after_anthesisseedZhengmai119miRNA_HN27D3China:Zhengzhoudifferent nitrogen level
SRR10051493cDNAZ_27D_227_days_after_anthesisseedZhengmai119miRNA_HN27D2China:Zhengzhoudifferent nitrogen level
SRR10051494cDNAZ_27D_127_days_after_anthesisseedZhengmai119miRNA_HN27D1China:Zhengzhoudifferent nitrogen level
SRR10051495cDNAN0_17D_317_days_after_anthesisseedZhengmai119miRNA_LN17D3China:Zhengzhoudifferent nitrogen level
SRR10051496cDNAN0_17D_217_days_after_anthesisseedZhengmai119miRNA_LN17D2China:Zhengzhoudifferent nitrogen level
SRR10051497cDNAZ_6D_26_days_after_anthesisseedZhengmai119miRNA_HN6D2China:Zhengzhou-
SRR10051498cDNAZ_6D_16_days_after_anthesisseedZhengmai119miRNA_HN6D1China:Zhengzhoudifferent nitrogen level

Run Library Name Age Tissue Cultivar Sample Name Geographic Location Name Treatment
SRR9712609Tae Bgt 9622412 daysleafChinese SpringTae Bgt 96224Switzerland: ZurichBlumeria graminis infection
SRR9712610Tae Bgt 9420212 daysleafChinese SpringTae Bgt 94202Switzerland: ZurichBlumeria graminis infection
SRR9712611Tae Csuninf12 daysleafChinese SpringTae CsuninfSwitzerland: Zurich-
SRR9712612Tae Bgt JIW212 daysleafChinese SpringTae Bgt JIW2Switzerland: ZurichBlumeria graminis infection

Run Library Selection Stage Tissue Cultivar Sample Name Geographic Location Name
SRR9696790RT-PCRlate double ridge stageyoung spikesguomai301WT-ys-S2aChina:Zhengzhou
SRR9696791RT-PCRlate double ridge stageyoung stem tipsguomai301WT-st-S1cChina:Zhengzhou
SRR9696792RT-PCRlate double ridge stageyoung stem tipsguomai301WT-st-S1bChina:Zhengzhou
SRR9696793RT-PCRlate double ridge stageyoung stem tipsguomai301WT-st-S1aChina:Zhengzhou
SRR9696794RT-PCRlate double ridge stageyoung stem tipsguomai301ptsd1-st-S3bChina:Zhengzhou
SRR9696795RT-PCRlate double ridge stageyoung stem tipsguomai301ptsd1-st-S3aChina:Zhengzhou
SRR9696796RT-PCRlate double ridge stageyoung spikesguomai301WT-ys-S2cChina:Zhengzhou
SRR9696797RT-PCRlate double ridge stageyoung spikesguomai301WT-ys-S2bChina:Zhengzhou
SRR9696798RT-PCRlate double ridge stageyoung spikesguomai301ptsd1-ys-S4aChina:Zhengzhou
SRR9696799RT-PCRlate double ridge stageyoung stem tipsguomai301ptsd1-st-S3cChina:Zhengzhou
SRR9696800RT-PCRlate double ridge stageyoung spikesguomai301ptsd1-ys-S4cChina:Zhengzhou
SRR9696801RT-PCRlate double ridge stageyoung spikesguomai301ptsd1-ys-S4bChina:Zhengzhou

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name
SRR9648458RT-PCRys_1_miRNAspike differentiation stage 5young spikesGuomai 301ys_1_miRNAChina:Zhengzhou
SRR9648459RT-PCRys_3_miRNAspike differentiation stage 5young spikesGuomai 301ys_3_miRNAChina:Zhengzhou
SRR9648460RT-PCRys_2_miRNAspike differentiation stage 5young spikesGuomai 301ys_2_miRNAChina:Zhengzhou
SRR9648461RT-PCRst_2_miRNAspike differentiation stage 5stem tipsGuomai 301st_2_miRNAChina:Zhengzhou
SRR9648462RT-PCRst_1_miRNAspike differentiation stage 5stem tipsGuomai 301st_1_miRNAChina:Zhengzhou
SRR9648463RT-PCRtp_1_miRNA3-leaf to 4-leaf stagetiller primordiaGuomai 301tp_1_miRNAChina:Zhengzhou
SRR9648464RT-PCRst_3_miRNAspike differentiation stage 5stem tipsGuomai 301st_3_miRNAChina:Zhengzhou
SRR9648465RT-PCRtp_3_miRNA3-leaf to 4-leaf stagetiller primordiaGuomai 301tp_3_miRNAChina:Zhengzhou
SRR9648466RT-PCRtp_2_miRNA3-leaf to 4-leaf stagetiller primordiaGuomai 301tp_2_miRNAChina:Zhengzhou

Run Library Selection Genotype Stage Tissue Cultivar Sample Name
SRR8467485size fractionationwild typejointing stageleafwinter wheat Shimai 22GSM3573290
SRR8467484size fractionationwild typespring greenup stageleafwinter wheat Shimai 22GSM3573289
SRR8467483size fractionationwild typewinter dormancy stageleafwinter wheat Shimai 22GSM3573288
SRR8467482size fractionationwild typewinter wheat collected at three leaf stageleafwinter wheat Shimai 22GSM3573287

Run Library Selection Library Name Sample Name
ERR2777611size fractionationL001_R1_001SAMEA4883093
ERR2777610size fractionationL001_R1_001SAMEA4883092
ERR2777609size fractionationL001_R1_001SAMEA4883091
ERR2777608size fractionationL001_R1_001SAMEA4883090
ERR2777607size fractionationL001_R1_001SAMEA4883089
ERR2777606size fractionationL001_R1_001SAMEA4883088
ERR2777605size fractionationL001_R1_001SAMEA4883087
ERR2777604size fractionationL001_R1_001SAMEA4883086
ERR2777603size fractionationL001_R1_001SAMEA4883085
ERR2777602size fractionationL001_R1_001SAMEA4883084
ERR2777601size fractionationL001_R1_001SAMEA4883083
ERR2777600size fractionationL001_R1_001SAMEA4883082
ERR2777599size fractionationL001_R1_001SAMEA4883081
ERR2777598size fractionationL001_R1_001SAMEA4883080
ERR2777597size fractionationL001_R1_001SAMEA4883079

Run Library Name Ecotype Stage Tissue Cultivar Sample Name Geographic Location Name Lat_Lon Treatment Replicate
SRR8612151LK120hexaploidthree leaf stagerootKenong9204sample_6_deficient_K_120h_rootChina38.82 N 115.44 Edeficient-K treatment for 120hLK_120
SRR8612152LK48hexaploidthree leaf stagerootKenong9204sample_5_deficient_K_48h_rootChina38.82 N 115.44 Edeficient-K treatment for 48hLK_48
SRR8612153LK6hexaploidthree leaf stagerootKenong9204sample_2_deficient_K_6h_rootChina38.82 N 115.44 Edeficient-K treatment for 6hLK_6
SRR8612154CKhexaploidthree leaf stagerootKenong9204sample_1_deficient_K_0h_rootChina38.82 N 115.44 Edeficient-K treatment for 0hCK
SRR8612155LK24hexaploidthree leaf stagerootKenong9204sample_4_deficient_K_24h_rootChina38.82 N 115.44 Edeficient-K treatment for 24hLK_24
SRR8612156LK12hexaploidthree leaf stagerootKenong9204sample_3_deficient_K_12h_rootChina38.82 N 115.44 Edeficient-K treatment for 12hLK_12

Run Library Selection Library Name phenotype Stage Tissue Cultivar Sample Name Geographic Location Name Treatment Replicate
SRR8432572RT-PCRWSB_sRNA-2Radult-plant stagespikeletsWangshuibaiWSB_sRNA-2China: JiangsuFusarium head blightWSB_small_RNA_library2
SRR8432573RT-PCRWSB_sRNA-1Radult-plant stagespikeletsWangshuibaiWSB_sRNA-1China: JiangsuFusarium head blightWSB_small_RNA_library1
SRR8432587RT-PCRSumai3_sRNA-2Radult-plant stagespikeletsSumai_3Sumai3_sRNA-2China: JiangsuFusarium head blightSumai3_small_RNA_library2
SRR8432590RT-PCRSumai3_sRNA-1Radult-plant stagespikeletsSumai_3Sumai3_sRNA-1China: JiangsuFusarium head blightSumai3_small_RNA_library1

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name
SRR8374804cDNAT3seedling stageleavesXiaoyan 6Triticum aestivum-leavesChina:shannxi
SRR8374805cDNAT2seedling stageleavesXiaoyan 6Triticum aestivum-leavesChina:shannxi
SRR8374806cDNAT1seedling stageleavesXiaoyan 6Triticum aestivum-leavesChina:shannxi

Run Library Selection Library Name Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR7550252RANDOMwheat_mock_14dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550253RANDOMwheat_mock_12dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550254RANDOMwheat_mock_14dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550255RANDOMwheat_mock_14dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550256RANDOMwheat_mock_7dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550257RANDOMwheat_mock_7dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550258RANDOMwheat_mock_12dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550259RANDOMwheat_mock_12dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550270RANDOM3D7_14dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550271RANDOM3D7_14dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550272RANDOM3D7_12dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550273RANDOM3D7_12dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550274RANDOM3D7_12dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550275RANDOM3D7_7dpi_rep3LeavesDrifterwheatSwitzerlandrep3
SRR7550276RANDOM3D7_7dpi_rep2LeavesDrifterwheatSwitzerlandrep2
SRR7550277RANDOM3D7_7dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550278RANDOMwheat_mock_7dpi_rep1LeavesDrifterwheatSwitzerlandrep1
SRR7550279RANDOM3D7_14dpi_rep3LeavesDrifterwheatSwitzerlandrep3

Run Library Selection Biome Sample Name Geographic Location Name Environment
ERR2235370RANDOMrachisSAMEA104455176Canadagreenhouse
ERR2235369RANDOMrachisSAMEA104455175Canadagreenhouse
ERR2235368RANDOMrachisSAMEA104455174Canadagreenhouse
ERR2235367RANDOMrachisSAMEA104455173Canadagreenhouse
ERR2235366RANDOMrachisSAMEA104455172Canadagreenhouse
ERR2235365RANDOMrachisSAMEA104455171Canadagreenhouse
ERR2235364RANDOMspikeletSAMEA104455170Canadagreenhouse
ERR2235363RANDOMspikeletSAMEA104455169Canadagreenhouse
ERR2235362RANDOMspikeletSAMEA104455168Canadagreenhouse
ERR2235361RANDOMspikeletSAMEA104455167Canadagreenhouse
ERR2235360RANDOMspikeletSAMEA104455166Canadagreenhouse
ERR2235359RANDOMspikeletSAMEA104455165Canadagreenhouse
ERR2235358RANDOMrachisSAMEA104455164Canadagreenhouse
ERR2235357RANDOMrachisSAMEA104455163Canadagreenhouse
ERR2235356RANDOMrachisSAMEA104455162Canadagreenhouse
ERR2235355RANDOMrachisSAMEA104455161Canadagreenhouse
ERR2235354RANDOMrachisSAMEA104455160Canadagreenhouse
ERR2235353RANDOMrachisSAMEA104455159Canadagreenhouse
ERR2235352RANDOMspikeletSAMEA104455158Canadagreenhouse
ERR2235351RANDOMspikeletSAMEA104455157Canadagreenhouse
ERR2235350RANDOMspikeletSAMEA104455156Canadagreenhouse
ERR2235349RANDOMspikeletSAMEA104455155Canadagreenhouse
ERR2235348RANDOMspikeletSAMEA104455154Canadagreenhouse
ERR2235347RANDOMspikeletSAMEA104455153Canadagreenhouse

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Treatment
SRR6328644RT-PCRSR4Athree leaves stagerootSR4sRNAseq_9China:shandongpH8.9
SRR6328645RT-PCRJNAthree leaves stagerootJN177sRNAseq_3China:shandongpH8.9
SRR6328646RT-PCRSR3Cthree leaves stagerootSR3sRNAseq_4China:shandongcontrol
SRR6328647RT-PCRJNCthree leaves stagerootJN177sRNAseq_1China:shandongcontrol
SRR6328648RT-PCRJNSthree leaves stagerootJN177sRNAseq_2China:shandong200mM NaCl
SRR6328649RT-PCRSR4Cthree leaves stagerootSR4sRNAseq_7China:shandongcontrol
SRR6328650RT-PCRSR4Sthree leaves stagerootSR4sRNAseq_8China:shandong200mM NaCl
SRR6328651RT-PCRSR3Sthree leaves stagerootSR3sRNAseq_5China:shandong200mM NaCl
SRR6328652RT-PCRSR3Athree leaves stagerootSR3sRNAseq_6China:shandongpH8.9

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Replicate
SRR5944333size fractionationWT-sRNA-1HAI-rep11-12 HAIgerminating seedJimai5265WT_JMrep1
SRR5944334size fractionationWT-sRNA-1HAI-rep21-12 HAIgerminating seedJimai5265WT_JMrep2
SRR5944335size fractionationWT-sRNA-6HAI-rep11-12 HAIgerminating seedJimai5265WT_JMrep1
SRR5944336size fractionationWT-sRNA-6HAI-rep21-12 HAIgerminating seedJimai5265WT_JMrep2
SRR5944337size fractionationWT-sRNA-12HAI-rep11-12 HAIgerminating seedJimai5265WT_JMrep1
SRR5944338size fractionationWT-sRNA-12HAI-rep21-12 HAIgerminating seedJimai5265WT_JMrep2

Run Library Selection Stage Tissue Sample Name Geographic Location Name Treatment
SRR5816454RANDOM24hrootwr_173China:BeijingCa2+-channel blocker

Run Library Selection Stage Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR5461177cDNATSSpikeChinese SpringTS-2Chinabiological replicate 2
SRR5461176cDNATSSpikeChinese SpringTS-1Chinabiological replicate 1
SRR5460972cDNAAMSpikeChinese SpringAM-2Chinabiological replicate 2
SRR5460967cDNAAMSpikeChinese SpringAM-1Chinabiological replicate 1
SRR5460949cDNAFMSpikeChinese SpringFM-2Chinabiological replicate 2
SRR5460941cDNAFMSpikeChinese SpringFM-1Chinabiological replicate 1
SRR5460939cDNADRSpikeChinese SpringDR-2Chinabiological replicate 2
SRR5460930cDNADRSpikeChinese SpringDR-1Chinabiological replicate 1

Run Library Name Stage Tissue Cultivar Sample Name Geographic Location Name
SRR5381543CSAantherantherChinese SpringTriticum aestivum L. Chinese Spring (CS)China
SRR5381542CS-3CAantherantherChinese SpringChinese Spring-gametocidal 3C chromosome monosomic addition line (CS-3C)China

Run Library Selection Library Name Age Tissue Cultivar Sample Name Geographic Location Name Treatment Replicate
SRR5270350size fractionation12dpi_C_R117 daysFirst expanded leafProINTA Federal12dpi_C_R1Argentina: CordobaControl 12 dpi Replicate 1Replicate 1
SRR5270349size fractionation12dpi_C_R217 daysFirst expanded leafProINTA Federal12dpi_C_R2Argentina: CordobaControl 12 dpi Replicate 2Replicate 2
SRR5270348size fractionation12dpi_T_R117 daysFirst expanded leafProINTA Federal12dpi_T_R1Argentina: CordobaInfected 12 dpi Replicate 1Replicate 1
SRR5270347size fractionation12dpi_T_R217 daysFirst expanded leafProINTA Federal12dpi_T_R2Argentina: CordobaInfected 12 dpi Replicate 2Replicate 2
SRR5270346size fractionation21dpi_C_R126 daysFirst expanded leafProINTA Federal21dpi_C_R1Argentina: CordobaControl 21 dpi Replicate 1Replicate 1
SRR5270345size fractionation21dpi_C_R226 daysFirst expanded leafProINTA Federal21dpi_C_R2Argentina: CordobaControl 21 dpi Replicate 2Replicate 2
SRR5270344size fractionation21dpi_T_R126 daysFirst expanded leafProINTA Federal21dpi_T_R1Argentina: CordobaInfected 21 dpi Replicate 1Replicate 1
SRR5270343size fractionation21dpi_T_R226 daysFirst expanded leafProINTA Federal21dpi_T_R2Argentina: CordobaInfected 21 dpi Replicate 2Replicate 2

Run Library Selection Library Name Age Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR5096892size fractionationsRNA_DDAA130 daysLeaf 30DTQ27 X TMU38sRNA_DDAA1China:Beijingrep1
SRR5096890size fractionationsRNA_DDAA230 daysLeaf 31DTQ27 X TMU38sRNA_DDAA2China:Beijingrep2
SRR5096872size fractionationsRNA_AADD130 daysLeaf 32DTMU38 X TQ27sRNA_AADD1China:Beijingrep1
SRR5096869size fractionationsRNA_AADD230 daysLeaf 33DTMU38 X TQ27sRNA_AADD2China:Beijingrep2
SRR5096896size fractionationsRNA_AA230 daysLeaf 30DTMU38sRNA_AA2China:Beijing-
SRR5096879size fractionationsRNA_AA130 daysLeaf 30DTMU38sRNA_AA1China:Beijing-
SRR5096877size fractionationsRNA_DD130 daysLeaf 30DTQ27sRNA_DD1China:Beijing-
SRR5096871size fractionationsRNA_DD230 daysLeaf 30DTQ27sRNA_DD2China:Beijing-

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR4417233size fractionationL21AMature seedSeedL21L21Chinarep1
SRR4417232size fractionationJ22CMature seedSeedJ22J22Chinarep3
SRR4417231size fractionationJ22BMature seedSeedJ22J22Chinarep2
SRR4417230size fractionationJ22AMature seedSeedJ22J22Chinarep1
SRR4417229size fractionationJ19CMature seedSeedJ19J19Chinarep3
SRR4417228size fractionationJ19BMature seedSeedJ19J19Chinarep2
SRR4417227size fractionationJ19AMature seedSeedJ19J19Chinarep1
SRR4417226size fractionationT349CMature seedSeedT349T349Chinarep3
SRR4417225size fractionationL21CMature seedSeedL21L21Chinarep3
SRR4417224size fractionationL21BMature seedSeedL21L21Chinarep2
SRR4417223size fractionationT349BMature seedSeedT349T349Chinarep2
SRR4417222size fractionationT349AMature seedSeedT349T349Chinarep1

Run Library Selection Library Name genotype Stage Tissue Phenotype Sample Name Geographic Location Name Replicate
SRR4114480RANDOM7294_sRNASeq7294bootingLeaf sheathglaucous7294_sRNASeqCanada: Saskatchewan-
SRR4114479RANDOM7293_sRNASeq7293bootingLeaf sheathglaucous7293_sRNASeqCanada: Saskatchewan-
SRR4114478RANDOM7290_sRNASeq7290bootingLeaf sheathglaucous7290_sRNASeqCanada: Saskatchewan-
SRR4114477RANDOM7289_sRNASeq7289bootingLeaf sheathglaucous7289_sRNASeqCanada: Saskatchewan-
SRR4114476RANDOM7287_sRNASeq7287bootingLeaf sheathnon-glaucous7287_sRNASeqCanada: Saskatchewan-
SRR4114475RANDOM7285_sRNASeq7285bootingLeaf sheathnon-glaucous7285_sRNASeqCanada: Saskatchewan-
SRR4114473RANDOM7282_sRNASeq7282bootingLeaf sheathnon-glaucous7282_sRNASeqCanada: Saskatchewan-
SRR4114472RANDOM7281_sRNASeq7281bootingLeaf sheathnon-glaucous7281_sRNASeqCanada: Saskatchewan-
SRR4114471RANDOM7279_sRNASeq7279bootingLeaf sheathnon-glaucous7279_sRNASeqCanada: Saskatchewan-
SRR4114460RANDOMD051N-2_sRNASeqD051NbootingLeaf sheathnon-glaucousD051N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114459RANDOMD051N-1_sRNASeqD051NbootingLeaf sheathnon-glaucousD051N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114458RANDOMD051-2_sRNASeqD051bootingLeaf sheathglaucousD051-2_sRNASeqCanada: Saskatchewanrep2
SRR4114457RANDOMD051-1_sRNASeqD051bootingLeaf sheathglaucousD051-1_sRNASeqCanada: Saskatchewanrep1
SRR4114456RANDOMAG2N-4_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-4_sRNASeqCanada: Saskatchewanrep4
SRR4114455RANDOMAG2N-3_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-3_sRNASeqCanada: Saskatchewanrep3
SRR4114454RANDOMAG2N-2_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114453RANDOMAG2N-1_sRNASeqAG2NbootingLeaf sheathnon-glaucousAG2N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114451RANDOMAG2F1-2_sRNASeqAG2F1bootingLeaf sheathnon-glaucousAG2F1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114450RANDOMAG2F1-1_sRNASeqAG2F1bootingLeaf sheathnon-glaucousAG2F1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114449RANDOMAG2-4_sRNASeqAG2bootingLeaf sheathglaucousAG2-4_sRNASeqCanada: Saskatchewanrep4
SRR4114448RANDOMAG2-3_sRNASeqAG2bootingLeaf sheathglaucousAG2-3_sRNASeqCanada: Saskatchewanrep3
SRR4114447RANDOMAG2-2_sRNASeqAG2bootingLeaf sheathglaucousAG2-2_sRNASeqCanada: Saskatchewanrep2
SRR4114446RANDOMAG2-2_F1C_sRNASeqAG2_F1CbootingLeaf sheathglaucousAG2-2_F1C_sRNASeqCanada: Saskatchewanrep2
SRR4114445RANDOMAG2-1_sRNASeqAG2bootingLeaf sheathglaucousAG2-1_sRNASeqCanada: Saskatchewanrep1
SRR4114444RANDOMAG2-1_F1C_sRNASeqAG2_F1CbootingLeaf sheathglaucousAG2-1_F1C_sRNASeqCanada: Saskatchewanrep1
SRR4114443RANDOMAG1N-2_sRNASeqAG1NbootingLeaf sheathnon-glaucousAG1N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114442RANDOMAG1N-1_sRNASeqAG1NbootingLeaf sheathnon-glaucousAG1N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114440RANDOMAG1F1-2_sRNASeqAG1F1bootingLeaf sheathnon-glaucousAG1F1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114439RANDOMAG1F1-1_sRNASeqAG1F1bootingLeaf sheathnon-glaucousAG1F1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114438RANDOMAG1-2_sRNASeqAG1bootingLeaf sheathglaucousAG1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114437RANDOMAG1-1_sRNASeqAG1bootingLeaf sheathglaucousAG1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114436RANDOMAE3N-2_sRNASeqAE3NbootingLeaf sheathnon-glaucousAE3N-2_sRNASeqCanada: Saskatchewanrep2
SRR4114435RANDOMAE3N-1_sRNASeqAE3NbootingLeaf sheathnon-glaucousAE3N-1_sRNASeqCanada: Saskatchewanrep1
SRR4114434RANDOMAE3F1-2_sRNASeqAE3F1bootingLeaf sheathnon-glaucousAE3F1-2_sRNASeqCanada: Saskatchewanrep2
SRR4114433RANDOMAE3F1-1_sRNASeqAE3F1bootingLeaf sheathnon-glaucousAE3F1-1_sRNASeqCanada: Saskatchewanrep1
SRR4114432RANDOMAE3-2_sRNASeqAE3bootingLeaf sheathglaucousAE3-2_sRNASeqCanada: Saskatchewanrep2
SRR4114431RANDOMAE3-1_sRNASeqAE3bootingLeaf sheathglaucousAE3-1_sRNASeqCanada: Saskatchewanrep1

Run Library Selection SAMPLING_TIME Cultivar Sample Name Treatment Replicate
SRR3721412size fractionation10 day after treatment (DAT)GlenleaGSM2214310UVRep3
SRR3721411size fractionation10 day after treatment (DAT)GlenleaGSM2214309LightRep3
SRR3721410size fractionation10 day after treatment (DAT)GlenleaGSM2214308HeatRep3
SRR3721409size fractionation10 day after treatment (DAT)GlenleaGSM2214307ControlRep3
SRR3721408size fractionation10 day after treatment (DAT)GlenleaGSM2214306UVRep2
SRR3721407size fractionation10 day after treatment (DAT)GlenleaGSM2214305LightRep2
SRR3721406size fractionation10 day after treatment (DAT)GlenleaGSM2214304HeatRep2
SRR3721405size fractionation10 day after treatment (DAT)GlenleaGSM2214303ControlRep2
SRR3721404size fractionation10 day after treatment (DAT)GlenleaGSM2214302UVRep1
SRR3721403size fractionation10 day after treatment (DAT)GlenleaGSM2214301LightRep1
SRR3721402size fractionation10 day after treatment (DAT)GlenleaGSM2214300HeatRep1
SRR3721401size fractionation10 day after treatment (DAT)GlenleaGSM2214299ControlRep1
SRR3721400size fractionation7 day after treatment (DAT)GlenleaGSM2214298UVRep3
SRR3721399size fractionation7 day after treatment (DAT)GlenleaGSM2214297LightRep3
SRR3721398size fractionation7 day after treatment (DAT)GlenleaGSM2214296HeatRep3
SRR3721397size fractionation7 day after treatment (DAT)GlenleaGSM2214295ControlRep3
SRR3721396size fractionation7 day after treatment (DAT)GlenleaGSM2214294UVRep2
SRR3721395size fractionation7 day after treatment (DAT)GlenleaGSM2214293LightRep2
SRR3721394size fractionation7 day after treatment (DAT)GlenleaGSM2214292HeatRep2
SRR3721393size fractionation7 day after treatment (DAT)GlenleaGSM2214291ControlRep2
SRR3721392size fractionation7 day after treatment (DAT)GlenleaGSM2214290UVRep1
SRR3721391size fractionation7 day after treatment (DAT)GlenleaGSM2214289LightRep1
SRR3721390size fractionation7 day after treatment (DAT)GlenleaGSM2214288HeatRep1
SRR3721389size fractionation7 day after treatment (DAT)GlenleaGSM2214287ControlRep1
SRR3721388size fractionation3 day after treatment (DAT)GlenleaGSM2214286UVRep3
SRR3721387size fractionation3 day after treatment (DAT)GlenleaGSM2214285LightRep3
SRR3721386size fractionation3 day after treatment (DAT)GlenleaGSM2214284HeatRep3
SRR3721385size fractionation3 day after treatment (DAT)GlenleaGSM2214283ControlRep3
SRR3721384size fractionation3 day after treatment (DAT)GlenleaGSM2214282UVRep2
SRR3721383size fractionation3 day after treatment (DAT)GlenleaGSM2214281LightRep2
SRR3721382size fractionation3 day after treatment (DAT)GlenleaGSM2214280HeatRep2
SRR3721381size fractionation3 day after treatment (DAT)GlenleaGSM2214279ControlRep2
SRR3721380size fractionation3 day after treatment (DAT)GlenleaGSM2214278UVRep1
SRR3721379size fractionation3 day after treatment (DAT)GlenleaGSM2214277LightRep1
SRR3721378size fractionation3 day after treatment (DAT)GlenleaGSM2214276HeatRep1
SRR3721377size fractionation3 day after treatment (DAT)GlenleaGSM2214275ControlRep1
SRR3721376size fractionation2 day after treatment (DAT)GlenleaGSM2214274UVRep3
SRR3721375size fractionation2 day after treatment (DAT)GlenleaGSM2214273LightRep3
SRR3721374size fractionation2 day after treatment (DAT)GlenleaGSM2214272HeatRep3
SRR3721373size fractionation2 day after treatment (DAT)GlenleaGSM2214271ControlRep3
SRR3721372size fractionation2 day after treatment (DAT)GlenleaGSM2214270UVRep2
SRR3721371size fractionation2 day after treatment (DAT)GlenleaGSM2214269LightRep2
SRR3721370size fractionation2 day after treatment (DAT)GlenleaGSM2214268HeatRep2
SRR3721369size fractionation2 day after treatment (DAT)GlenleaGSM2214267ControlRep2
SRR3721368size fractionation2 day after treatment (DAT)GlenleaGSM2214266UVRep1
SRR3721367size fractionation2 day after treatment (DAT)GlenleaGSM2214265LightRep1
SRR3721366size fractionation2 day after treatment (DAT)GlenleaGSM2214264HeatRep1
SRR3721365size fractionation2 day after treatment (DAT)GlenleaGSM2214263ControlRep1
SRR3721364size fractionation1 day after treatment (DAT)GlenleaGSM2214262UVRep3
SRR3721363size fractionation1 day after treatment (DAT)GlenleaGSM2214261LightRep3
SRR3721362size fractionation1 day after treatment (DAT)GlenleaGSM2214260HeatRep3
SRR3721361size fractionation1 day after treatment (DAT)GlenleaGSM2214259ControlRep3
SRR3721360size fractionation1 day after treatment (DAT)GlenleaGSM2214258UVRep2
SRR3721359size fractionation1 day after treatment (DAT)GlenleaGSM2214257LightRep2
SRR3721358size fractionation1 day after treatment (DAT)GlenleaGSM2214256HeatRep2
SRR3721357size fractionation1 day after treatment (DAT)GlenleaGSM2214255ControlRep2
SRR3721356size fractionation1 day after treatment (DAT)GlenleaGSM2214254UVRep1
SRR3721355size fractionation1 day after treatment (DAT)GlenleaGSM2214253LightRep1
SRR3721354size fractionation1 day after treatment (DAT)GlenleaGSM2214252HeatRep1
SRR3721353size fractionation1 day after treatment (DAT)GlenleaGSM2214251ControlRep1
SRR3721352size fractionation0 day after treatment (DAT)GlenleaGSM2214250UVRep3
SRR3721351size fractionation0 day after treatment (DAT)GlenleaGSM2214249LightRep3
SRR3721350size fractionation0 day after treatment (DAT)GlenleaGSM2214248HeatRep3
SRR3721349size fractionation0 day after treatment (DAT)GlenleaGSM2214247ControlRep3
SRR3721348size fractionation0 day after treatment (DAT)GlenleaGSM2214246UVRep2
SRR3721347size fractionation0 day after treatment (DAT)GlenleaGSM2214245LightRep2
SRR3721346size fractionation0 day after treatment (DAT)GlenleaGSM2214244HeatRep2
SRR3721345size fractionation0 day after treatment (DAT)GlenleaGSM2214243ControlRep2
SRR3721344size fractionation0 day after treatment (DAT)GlenleaGSM2214242UVRep1
SRR3721343size fractionation0 day after treatment (DAT)GlenleaGSM2214241LightRep1
SRR3721342size fractionation0 day after treatment (DAT)GlenleaGSM2214240HeatRep1
SRR3721341size fractionation0 day after treatment (DAT)GlenleaGSM2214239ControlRep1

Run Library Selection Stage Tissue Cultivar Sample Name Geographic Location Name
SRR3144539size fractionationafter 15 days culture derived from immature embryocallusZhoumai18IME15 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3144538size fractionationafter 6 days culture derived from immature embryocallusZhoumai18IME6 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3144523size fractionationafter 3 days culture derived from immature embryocallusZhoumai18IME3 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3144511size fractionationafter 6 days culture dereved from mature embryocallusZhoumai 18ME6 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3140978size fractionationafter15 days culture derived from mature embryowheat callusZhoumai 18ME15 sRNA libraryChina: Henan Agricultural University, Zhengzhou
SRR3140846size fractionationafter 3 days culture dereved from mature embryocallusZhoumai 18ME3 sRNA libraryChina: Henan Agricultural University, Zhengzhou

Experiment Accession Run Library Selection Age Tissue Cultivar Sample Name Geographic Location Name
SRX1548275SRR3151453 SRR3151454size fractionation30 daysgrainSpeltaI_Spelta_30DHungary
SRX1548274SRR3151451 SRR3151452size fractionation20 daysgrainSpeltaI_Spelta_20DHungary
SRX1548273SRR3151449 SRR3151450size fractionation10 daysgrainSpeltaI_Spelta_10DHungary
SRX1548272SRR3151447 SRR3151448size fractionation30 daysgrainBankuti lines B52I_B52_30DHungary
SRX1548271SRR3151445 SRR3151446size fractionation20 daysgrainBankuti lines B52I_B52_20DHungary
SRX1548269SRR3151443 SRR3151444size fractionation10 daysgrainBankuti lines B52I_B52_10DHungary
SRX1548268SRR3151441 SRR3151442size fractionation30 daysgrainBankuti lines B35I_B35_30DHungary
SRX1548266SRR3151439 SRR3151440size fractionation20 daysgrainBankuti lines B35I_B35_20DHungary
SRX1548265SRR3151437 SRR3151438size fractionation10 daysgrainBankuti lines B35I_B35_10DHungary
SRX1548199SRR3151435 SRR3151436RANDOM30 daysgrainSpeltaT_Spelta_30DHungary
SRX1548198SRR3151433 SRR3151434RANDOM20 daysgrainSpeltaT_Spelta_20DHungary
SRX1548192SRR3151431 SRR3151432RANDOM10 daysgrainSpeltaT_Spelta_10DHungary
SRX1548177SRR3151429 SRR3151430RANDOM30 daysgrainBankuti lines B52T_B52_30DHungary
SRX1548164SRR3151427 SRR3151428RANDOM20 daysgrainBankuti lines B52T_B52_20DHungary
SRX1548163SRR3151425 SRR3151426RANDOM10 daysgrainBankuti lines B52T_B52_10DHungary
SRX1548152SRR3144555 SRR3151424RANDOM30 daysgrainBankuti lines B35T_B35_30DHungary
SRX1548148SRR3144553 SRR3144554RANDOM20 daysgrainBankuti lines B35T_B35_20DHungary
SRX1546448SRR3144551 SRR3144552RANDOM10 daysgrainBankuti lines B35T_B35_10DHungary

Run Library Selection Library Name Stage Tissue Cultivar Sample Name Geographic Location Name Treatment
SRR3136704RANDOMSKS-Sseed germinationShootHD-2967Shoot tissueIndiaNitrate stress
SRR2146991RANDOMSKS-NSeedling stageRootHD-2967Root tisueIndiaNitrate stress
SRR2146992RANDOMSKS-SSeedling stageRootHD-2967Root tisueIndiaNitrate stress

Run Library Selection Age/Stage Tissue Cultivar Sample Name
SRR1796322size fractionation28 days post anthesisgrainChinese SpringGSM1606477
SRR1796321size fractionation21 days post anthesisgrainChinese SpringGSM1606476
SRR1796320size fractionation14 days post anthesisgrainChinese SpringGSM1606475
SRR1796319size fractionation7 days post anthesisgrainChinese SpringGSM1606474

Run Library Selection Stage Tissue Cultivar Sample Name
SRR1728653size fractionationtwo-leaf stageleafZhengyin1T2 sRNA library
SRR1728566size fractionationtwo-leaf stageleafZhengyin1C2 sRNA library
SRR1727004size fractionationtwo-leaf stageleafHanxuan10C1
SRR1728309size fractionationtwo-leaf stageleafHanxuan 10T1 sRNA library

Run Library Selection Library Name Age Tissue Cultivar Sample Name Geographic Location Name
SRR1646809size fractionationR-PImi_data.fastq10 daysleafHD2329+Lr24Small RNA Wheat HD2329+Lr24 infected with Puccinia triticina (R-PImi)India
SRR1646808size fractionationR-Mmi_data.fastq10 daysleafHD2329+Lr24Small RNA Wheat HD2329+Lr24 mock inoculated (R-Mmi)India
SRR1646807size fractionationS-PImi_data.fastq10 daysleafHD2329Small RNA Wheat HD2329 infected with Puccinia triticina (S-PImi)India
SRR1646806size fractionationSmall RNA Wheat HD2329 mock inoculated (S-Mmi)10 daysleafHD2329Small RNA Wheat HD2329 mock inoculated (S-Mmi)India

Experiment Accession Run Cultivar Sample Name
SRX500281SRR1203861 SRR1203865 SRR1203916 SRR1203917wheatIdentification of Novel miRNAs and miRNA Expression Profiling in Wheat Hybrid Necrosis

Run Library Selection Tissue Cultivar Sample Name
SRR1196023size fractionationflag leavesChinese SpringFL
SRR1196045size fractionationgrain of 15 days after pollinationChinese SpringGRA15
SRR1196029size fractionationgrain of 8 days after pollinationChinese SpringGRA8
SRR1196022size fractionation10-15 mm young spikesChinese SpringYS15
SRR1195944size fractionation0-5 mm young spikesChinese SpringYS5
SRR1195282size fractionationstems in the jointing stageChinese SpringSJ
SRR1195281size fractionationseedling rootChinese SpringSR
SRR1195280size fractionationseedling leafChinese Springseedling leaf
SRR1195279size fractionationseedling shootChinese SpringSH
SRR1195025size fractionationembryo of germinating seed embryosChinese SpringGSE
SRR1195024size fractionationgrainsChinese Springdry grains

Run Library Selection Sample Name
SRR800158RANDOM PCRHD2985_treated
SRR800157RANDOMHD2985_control

Run Library Name Library Selection Ecotype Stage Tissue Sample Name Geographic Location Name Treatment
SRR22878362AGA-sRNAPCRGGAAxAA-F1three-leafseedlingSample 1Chinasmall RNA
SRR22878361AGD-sRNAPCRGGAAxDD-F1three-leafseedlingSample 2Chinasmall RNA

Run Library Selection Stage Tissue Cultivar Sample Name Geographic Location Name
SRR5460520RT-PCR6 days after pollinationendospermTMU06TMU06(AA)_endosperm_sRNAChina:Nanjing
SRR5460519RT-PCR6 days after pollinationendospermTMU06TMU06(AA)_endosperm_sRNAChina:Nanjing
SRR5460518RT-PCR6 days after pollinationendospermTMU06TMU06(AA)_endosperm_sRNAChina:Nanjing
SRR5460508RT-PCR6 days after pollinationendospermTMU38TMU38(AA)_endosperm_sRNAChina:Nanjing
SRR5460507RT-PCR6 days after pollinationendospermTMU38TMU38(AA)_endosperm_sRNAChina:Nanjing
SRR5460481RT-PCR6 days after pollinationendospermTMU38TMU38(AA)_endosperm_sRNAChina:Nanjing
SRR5460523RT-PCR6 days after pollinationendospermTL05TL05(SS)_endosperm_sRNAChina:Nanjing
SRR5460522RT-PCR6 days after pollinationendospermTL05TL05(SS)_endosperm_sRNAChina:Nanjing
SRR5460521RT-PCR6 days after pollinationendospermTL05TL05(SS)_endosperm_sRNAChina:Nanjing
SRR5460513RT-PCR6 days after pollinationendospermTQ27TQ27(DD)_endosperm_sRNAChina:Nanjing
SRR5460512RT-PCR6 days after pollinationendospermTQ27TQ27(DD)_endosperm_sRNAChina:Nanjing
SRR5460509RT-PCR6 days after pollinationendospermTQ27TQ27(DD)_endosperm_sRNAChina:Nanjing
SRR5460516RT-PCR6 days after pollinationendospermAT2AT2(AADD)_endosperm_sRNAChina:Nanjing
SRR5460517RT-PCR6 days after pollinationendospermAT2AT2(AADD)_endosperm_sRNAChina:Nanjing
SRR5460524RT-PCR6 days after pollinationendospermTLTUTLTU(SSAA)_endosperm_sRNAChina:Nanjing
SRR5460526RT-PCR6 days after pollinationendospermTLTUTLTU(SSAA)_endosperm_sRNAChina:Nanjing
SRR5460528RT-PCR6 days after pollinationendospermTLTUTLTU(SSAA)_endosperm_sRNAChina:Nanjing
SRR5460515RT-PCR6 days after pollinationendospermAT2AT2(AADD)_endosperm_sRNAChina:Nanjing

Run Library Name Age Tissue Cultivar Sample Name Geographic Location Name Replicate
SRR11881520Hvu-1.0-1_M_sRNA-seq1.0 mm antherAntherMorexHvu-1.0-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881531Hvu-0.8-3_M_sRNA-seq0.8 mm antherAntherMorexHvu-0.8-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881552Hvu-0.8-1_M_sRNA-seq0.8 mm antherAntherMorexHvu-0.8-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881563Hvu-0.6-3_M_sRNA-seq0.6 mm antherAntherMorexHvu-0.6-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881574Hvu-0.6-2_M_sRNA-seq0.6 mm antherAntherMorexHvu-0.6-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881585Hvu-0.6-1_M_sRNA-seq0.6 mm antherAntherMorexHvu-0.6-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881596Hvu-0.4-4_M_sRNA-seq0.4 mm antherAntherMorexHvu-0.4-4_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881605Hvu-1.8-3_GP_sRNA-seq1.8 mm antherAntherGolden PromiseHvu-1.8-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881606Hvu-1.8-2_GP_sRNA-seq1.8 mm antherAntherGolden PromiseHvu-1.8-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881607Hvu-0.4-2_M_sRNA-seq0.4 mm antherAntherMorexHvu-0.4-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881608Hvu-1.8-1_GP_sRNA-seq1.8 mm antherAntherGolden PromiseHvu-1.8-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881609Hvu-1.4-3_GP_sRNA-seq1.4 mm antherAntherGolden PromiseHvu-1.4-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881610Hvu-1.4-2_GP_sRNA-seq1.4 mm antherAntherGolden PromiseHvu-1.4-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881611Hvu-1.4-1_GP_sRNA-seq1.4 mm antherAntherGolden PromiseHvu-1.4-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881612Hvu-1.0-4_GP_sRNA-seq1.0 mm antherAntherGolden PromiseHvu-1.0-4_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881613Hvu-0.8-2_M_sRNA-seq0.8 mm antherAntherMorexHvu-0.8-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881614Hvu-0.2-2_M_sRNA-seq0.2 mm antherAntherMorexHvu-0.2-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881615Hvu-0.2-1_M_sRNA-seq0.2 mm antherAntherMorexHvu-0.2-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881616Hvu-1.0-2_GP_sRNA-seq1.0 mm antherAntherGolden PromiseHvu-1.0-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881617Hvu-1.0-1_GP_sRNA-seq1.0 mm antherAntherGolden PromiseHvu-1.0-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881618Hvu-0.8-3_GP_sRNA-seq0.8 mm antherAntherGolden PromiseHvu-0.8-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881619Hvu-0.8-2_GP_sRNA-seq0.8 mm antherAntherGolden PromiseHvu-0.8-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881620Hvu-0.8-1_GP_sRNA-seq0.8 mm antherAntherGolden PromiseHvu-0.8-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881621Hvu-0.4-1_M_sRNA-seq0.4 mm antherAntherMorexHvu-0.4-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881622Hvu-0.6-3_GP_sRNA-seq0.6 mm antherAntherGolden PromiseHvu-0.6-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881623Hvu-0.6-2_GP_sRNA-seq0.6 mm antherAntherGolden PromiseHvu-0.6-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881624Hvu-0.6-1_GP_sRNA-seq0.6 mm antherAntherGolden PromiseHvu-0.6-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881625Hvu-0.4-3_GP_sRNA-seq0.4 mm antherAntherGolden PromiseHvu-0.4-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881626Hvu-0.4-2_GP_sRNA-seq0.4 mm antherAntherGolden PromiseHvu-0.4-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881627Hvu-0.4-1_GP_sRNA-seq0.4 mm antherAntherGolden PromiseHvu-0.4-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881628Hvu-0.2-3_GP_sRNA-seq0.2 mm antherAntherGolden PromiseHvu-0.2-3_GP_sRNA-seqUSA: St. Louisreplicate 3
SRR11881629Hvu-0.2-2_GP_sRNA-seq0.2 mm antherAntherGolden PromiseHvu-0.2-2_GP_sRNA-seqUSA: St. Louisreplicate 2
SRR11881630Hvu-0.2-1_GP_sRNA-seq0.2 mm antherAntherGolden PromiseHvu-0.2-1_GP_sRNA-seqUSA: St. Louisreplicate 1
SRR11881631Hvu-1.8-3_M_sRNA-seq1.8 mm antherAntherMorexHvu-1.8-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881632Hvu-0.2-3_M_sRNA-seq0.2 mm antherAntherMorexHvu-0.2-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881633Hvu-1.8-2_M_sRNA-seq1.8 mm antherAntherMorexHvu-1.8-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881634Hvu-1.8-1_M_sRNA-seq1.8 mm antherAntherMorexHvu-1.8-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881635Hvu-1.4-4_M_sRNA-seq1.4 mm antherAntherMorexHvu-1.4-4_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881636Hvu-1.4-3_M_sRNA-seq1.4 mm antherAntherMorexHvu-1.4-3_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881637Hvu-1.4-1_M_sRNA-seq1.4 mm antherAntherMorexHvu-1.4-1_M_sRNA-seqUSA: St. Louisreplicate 1
SRR11881638Hvu-1.0-3_M_sRNA-seq1.0 mm antherAntherMorexHvu-1.0-3_M_sRNA-seqUSA: St. Louisreplicate 3
SRR11881639Hvu-1.0-2_M_sRNA-seq1.0 mm antherAntherMorexHvu-1.0-2_M_sRNA-seqUSA: St. Louisreplicate 2
SRR11881537Tae-1.8-3_F_sRNA-seq1.8 mm antherAntherFielderTae-1.8-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881538Tae-1.8-2_F_sRNA-seq1.8 mm antherAntherFielderTae-1.8-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881539Tae-1.8-1_F_sRNA-seq1.8 mm antherAntherFielderTae-1.8-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881540Tae-1.4-4_F_sRNA-seq1.4 mm antherAntherFielderTae-1.4-4_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881541Tae-1.4-3_F_sRNA-seq1.4 mm antherAntherFielderTae-1.4-3_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881542Tae-1.4-1_F_sRNA-seq1.0 mm antherAntherFielderTae-1.4-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881543Tae-1.0-3_F_sRNA-seq1.0 mm antherAntherFielderTae-1.0-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881544Tae-1.0-2_F_sRNA-seq1.0 mm antherAntherFielderTae-1.0-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881545Tae-1.0-1_F_sRNA-seq1.0 mm antherAntherFielderTae-1.0-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881546Tae-0.8-3_F_sRNA-seq0.8 mm antherAntherFielderTae-0.8-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881547Tae-0.8-2_F_sRNA-seq0.8 mm antherAntherFielderTae-0.8-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881548Tae-0.8-1_F_sRNA-seq0.8 mm antherAntherFielderTae-0.8-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881549Tae-0.6-3_F_sRNA-seq0.6 mm antherAntherFielderTae-0.6-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881550Tae-0.6-2_F_sRNA-seq0.6 mm antherAntherFielderTae-0.6-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881551Tae-0.6-1_F_sRNA-seq0.6 mm antherAntherFielderTae-0.6-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881553Tae-0.4-4_F_sRNA-seq0.4 mm antherAntherFielderTae-0.4-4_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881554Tae-0.4-2_F_sRNA-seq0.4 mm antherAntherFielderTae-0.4-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881555Tae-0.4-1_F_sRNA-seq0.4 mm antherAntherFielderTae-0.4-1_F_sRNA-seqUSA: St. Louisbiological replicate 1
SRR11881556Tae-0.2-3_F_sRNA-seq0.2 mm antherAntherFielderTae-0.2-3_F_sRNA-seqUSA: St. Louisbiological replicate 3
SRR11881557Tae-0.2-2_F_sRNA-seq0.2 mm antherAntherFielderTae-0.2-2_F_sRNA-seqUSA: St. Louisbiological replicate 2
SRR11881558Tae-0.2-1_F_sRNA-seq0.2 mm antherAntherFielderTae-0.2-1_F_sRNA-seqUSA: St. Louisbiological replicate 1